Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Blood cell staining method

A staining method and blood cell technology, applied in the preparation of test samples, etc., can solve the problems of light staining, unstable staining effect, unclear staining, etc., and achieve the effect of short time consumption

Inactive Publication Date: 2017-12-22
THE FIRST AFFILIATED HOSPITAL OF HENAN UNIV OF SCI & TECH
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The blood cell staining method provided by the present invention solves the problem that the staining effect caused by potassium dihydrogen phosphate-disodium hydrogen phosphate buffer as a diluent is unstable, and the staining is prone to unclear or shallow staining

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Blood cell staining method
  • Blood cell staining method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A blood cell staining method, comprising the following steps:

[0026] S1, preparation of staining solution

[0027] The staining solution was mixed with Wright's pigment, methanol and glycerin and then placed at room temperature for 3 days for later use. During the placement, it was shaken to promote the dissolution of Wright's pigment, wherein the ratio of Wright's pigment:methanol:glycerin was 1g:60ml:1ml;

[0028] S2, preparation of staining buffer

[0029] Each liter of staining buffer is prepared by mixing the following components: 0.3 g of potassium dihydrogen phosphate, 0.2 g of disodium hydrogen phosphate, 5 ml of glycerin, 5 μL of hydrogen peroxide with a mass fraction of 30%, distilled water to 1 L, and set aside;

[0030] S3, dyed

[0031] Dilute the staining solution of S1 with the staining buffer of S2, let it stand for 5 minutes after dilution, and mix well to obtain the diluted staining solution, and directly drop the diluted staining solution on the p...

Embodiment 2

[0037] A blood cell staining method, comprising the following steps:

[0038] S1, preparation of staining solution

[0039] The staining solution was mixed with Wright's pigment, methanol and glycerin and then placed at room temperature for 3 days for subsequent use. During the placement period, it was shaken to promote the dissolution of Wright's pigment, wherein the ratio of Wright's pigment: methanol: glycerol was 1g: 60ml: 2ml;

[0040] S2, preparation of staining buffer

[0041] Each liter of staining buffer is prepared by mixing the following components: 0.3 g of potassium dihydrogen phosphate, 0.2 g of disodium hydrogen phosphate, 10 ml of glycerin, 10 μL of hydrogen peroxide with a mass fraction of 30%, distilled water to 1 L, and set aside;

[0042] S3, dyed

[0043] Dilute the staining solution of S1 with the staining buffer of S2, let it stand for 5 minutes after dilution, and mix well to obtain the diluted staining solution, and directly drop the diluted staining...

Embodiment 3

[0049] A blood cell staining method, comprising the following steps:

[0050] S1, preparation of staining solution

[0051] The staining solution is mixed with Wright's pigment, methanol and glycerin and then placed at room temperature for 3 days for later use. During the placement period, it is shaken to promote the dissolution of Wright's pigment, wherein the ratio of Wright's pigment: methanol: glycerin is 1g: 60ml: 1.5ml ;

[0052] S2, preparation of staining buffer

[0053] Each liter of staining buffer is prepared by mixing the following components: 0.3 g of potassium dihydrogen phosphate, 0.2 g of disodium hydrogen phosphate, 7.5 ml of glycerin, 7.5 μL of hydrogen peroxide with a mass fraction of 30%, distilled water to 1 L, and set aside;

[0054] S3, dyed

[0055]Use the staining buffer of S2 to dilute the staining solution of S1, dilute and mix well and let it stand for 8 minutes to obtain the diluted staining solution. Add the diluted staining solution directly o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of blood cell staining and discloses a blood cell staining method. The method comprises the steps as follows: preparation of a staining solution, preparation of a staining buffer solution, staining and the like, wherein each liter of the staining buffer solution is prepared from 0.3 g of potassium dihydrogen phosphate, 0.2 g of disodium hydrogen phosphate, 5-10 ml of glycerol and 5-10 mu L of hydrogen peroxide with the mass fraction of 30% through mixing, and distilled water is added to the constant volume of 1L. According to the method, the staining buffer solution used in a Wright staining method is improved, hydrogen peroxide is added on the basis of the traditional buffer solution, production of azure is promoted under the action of oxidation of the hydrogen peroxide, so that after the prepared staining buffer solution and the staining solution are mixed, all that is required is to place an obtained mixture for 5-10 min, the mixture can be used, the staining effect is not affected, the staining solution only needs to be placed for 3 d after being prepared and can be used in cooperation with the staining buffer solution, and consumption time is short.

Description

technical field [0001] The invention belongs to the technical field of blood cell staining, and in particular relates to a blood cell staining method. Background technique [0002] Observing the shape of blood cells is a basic method in the medical examination process. If the cells are not stained, all the cells observed under the microscope have similar shapes, and it is difficult to distinguish blood cells. Therefore, Wright staining, Giemsa staining, Papanicolaou staining or hematoxylin-eosin staining are often used in the medical examination process for clinical staining of blood cells. [0003] Wright's staining method is currently the most commonly used and the simplest staining method. A neutral dye mixed with acid eosin and basic methylene blue in Wright's reagent, it will produce azure after a long period of time, which can stain the nucleus and cytoplasm. The dyeing principle of Wright's staining method has both physical adsorption and chemical affinity. Due to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 孙真真朱冰柯李丹丹
Owner THE FIRST AFFILIATED HOSPITAL OF HENAN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com