A TLC detection method of Chinese medicinal material Rhubarb palmatum

A detection method and technology of rhubarb palm leaf, applied in the direction of measuring device, material separation, analysis of materials, etc., can solve the problem of no report, etc., and achieve the effect of clear discrimination, easy discrimination, and good development effect.

Active Publication Date: 2020-11-20
浙江兰思教育科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, no effective method has been reported at home and abroad to identify rhubarb palmatum, rhubarb tangutica, and rhubarb medicinal

Method used

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  • A TLC detection method of Chinese medicinal material Rhubarb palmatum
  • A TLC detection method of Chinese medicinal material Rhubarb palmatum
  • A TLC detection method of Chinese medicinal material Rhubarb palmatum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 Detection method of the present invention

[0044] (1) Preparation of medicinal material solution

[0045] Take 0.75g of the rhubarb powder to be tested, accurately weigh it, put it in a conical flask with a stopper, add 10ml of 85% ethanol, ultrasonicate twice for 15 minutes each time, filter, combine the filtrates, evaporate the filtrate to dryness, add 10ml of water to the residue Dissolve, shake and extract 2 times with dichloromethane, 15ml each time, combine the dichloromethane solution, evaporate to dryness, add 1ml methanol to the residue to dissolve, and use it as the test solution.

[0046] (2) TLC detection

[0047] Take 3 μl of the above-mentioned test solution, use methanol as a blank solvent, spot on a silica gel plate, and develop with petroleum ether (30°C-60°C)-n-hexane-ethyl acetate-formic acid (4:12:6:0.2) Expand the agent, take it out, dry it, smoke it in ammonia vapor, and inspect it in sunlight.

[0048] (3) Result judgment

[0049...

experiment example 1

[0051] Experimental Example 1 Identification of Rhubarb palmatum

[0052] (1) Preparation of medicinal material solution

[0053]Take 0.75g of rhubarb palmatum, rhubarb tangutata and medicinal rhubarb powder respectively, weigh them precisely, put them in a stoppered conical flask, add 10ml of 85% ethanol, ultrasonicate twice for 15 minutes each time, filter and combine the filtrates , the filtrate was evaporated to dryness, the residue was dissolved by adding 10ml of water, extracted twice by shaking with dichloromethane, 15ml each time, the dichloromethane solution was combined, evaporated to dryness, and the residue was dissolved by adding 1ml of methanol as the test solution.

[0054] (2) TLC detection

[0055] Take 3 μl of the above-mentioned test solution, use methanol as the blank solvent, spot on the silica gel plate, and use petroleum ether (30°C-60°C)-n-hexane-ethyl acetate-formic acid (4:12:6:0.2) as the Expand the developer, take it out, dry it in the air, put it...

experiment example 2

[0060] The selection of experimental example 2 detection method developer of the present invention

[0061] (1) Preparation of the reference medicinal material solution

[0062] Take 0.75 g of rhubarb palmatum, rhubarb tanguta and medicinal rhubarb powder respectively, and prepare the reference substance solution according to the method in Example 1.

[0063] (2) TLC detection

[0064] Take 3 μl of the above solution and spot it on the silica gel H thin-layer plate with sodium carboxymethylcellulose as the binder, unfold it with the following expansion system respectively, take it out, dry it in the air, smoke it in ammonia vapor, and inspect it under sunlight, the thin layer Chromatogram see Figure 2-6 , wherein, the serial numbers 1-2 in the figure are rhubarb palmata, 3-4 are rhubarb tangutica, and 5-6 are rhubarb for medicinal use.

[0065] Expanding system I: acetone-methanol-n-hexane-dichloromethane (1:1:1:1);

[0066] Expanding system II: ethyl acetate-absolute eth...

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Abstract

The invention discloses a thin-layer chromatography detection method of rheum palmatum as a traditional Chinese medicinal material. The rheum palmatum is detected with thin-layer chromatography. The method comprises steps as follows: (1) preparation of a test liquid: taking test medicinal material powder, adding ethanol for extraction, performing filtering and concentrating, adding water for dissolution, adding an organic solvent for extraction, performing concentration, adding methanol to constant volume, and taking the solution as the test solution; (2) thin-layer chromatography detection: sampling the test solution on a thin-layer plate, performing developing with petroleum ether at 30-60 DEG C, n-hexane, ethyl acetate and formic acid as developers, and performing color development, inspection and contrast. According to the thin-layer chromatography detection method, purple characteristic spots are found in the Rf value range of 0.13-0.17 and are unique to the rheum palmatum, the spots are clear and easy to distinguish, and the rheum palmatum can be effectively distinguished from rheum palmatum, rheum tanguticum and rheum tanguticum.

Description

technical field [0001] The invention relates to a thin-layer chromatographic detection method for characteristic identification of Chinese medicinal material rhubarb palmatum. Background technique [0002] Thin-layer chromatography is to spot the solution of the test product on the thin-layer plate, develop it with a developing agent in a developing container, and compare the obtained chromatogram with the chromatogram obtained by the same method with a suitable standard substance, so as to identify and identify the drug. Methods of impurity inspection or content determination. It makes use of the different adsorption capacity of each component to the same adsorbent, so that in the process of the solvent flowing through the adsorbent, it will continuously produce adsorption, desorption, re-adsorption, and re-desorption, so that the components contained in the test sample are separated. Thin-layer chromatography has the advantages of simple equipment, easy operation, fast se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/94
CPCG01N30/94
Inventor 沈芃马晓玲曹晓红杜全胜卢福刘涛
Owner 浙江兰思教育科技有限公司
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