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Extraction method for microbial oil

A technology for microbial oil and microbial fermentation broth, which is applied in the production of fat, fat oil/fat refining, fat oil/fat production, etc., can solve the problems of affecting oil quality, high reaction temperature, environmental hazards, etc., to improve quality and avoid environmental pollution. , the effect of reducing refining consumption

Inactive Publication Date: 2017-12-29
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the solvent-free extraction process disclosed in this invention still needs to use a small amount of non-polar solvent, which will still cause harm and pollution to the environment
On the other hand, gravity centrifugal equipment such as tilting pen centrifuge will release a lot of heat when separating heavy phase and light phase, and the reaction temperature in the separation process is also high, which is likely to cause the oxidation of polyunsaturated fatty acids in oils, and then Affect oil quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Using Dinoflagellate as the fermentation strain, follow the steps below in turn:

[0018] (1) fermentation obtains the microbial fermentation liquid rich in docosahexaenoic acid;

[0019] (2) after above-mentioned fermented liquid is processed by centrifuge, obtaining solid content is 22% concentrated fermented liquid;

[0020] (3) take 1000g of the concentrated fermentation broth and put it into a 3L glass reaction flask, adjust the pH value to 11.5, add 0.5g of alkaline protease, control the temperature to 40°C, and enzymolysis for 5h to obtain a mixture after cell lysis;

[0021] (4) adding the mixture after the above-mentioned cell lysis into the membrane filtration equipment, circulating filtration, and separating the emulsified oil containing moisture;

[0022] (5) add the sodium chloride of 3.0% emulsifiable concentrate weight in the emulsified oil containing moisture, settling after stirring and mixing, get the supernatant to obtain 128.6g of microbial oils tha...

Embodiment 2

[0024] Using Micrococcus as a fermentation strain, follow the steps below in turn:

[0025] (1) fermentation obtains the microbial fermentation liquid rich in eicosapentaenoic acid;

[0026] (2) Take 35kg of the fermentation broth and put it into a 70L glass reactor, adjust the pH value to 10.8, add 21g of alkaline protease, control the temperature to 40°C, and perform a colloid mill cyclic shearing reaction for 3h to obtain a mixture after cell lysis.

[0027] (3) adding the mixture after the above-mentioned cell lysis to the membrane filtration equipment, circulating filtration, and separating the emulsified oil containing moisture;

[0028] (4) add the citric acid of 5.0% emulsifiable concentrate weight to the emulsified oil containing moisture, settling after stirring and mixing, get the supernatant to obtain 1788g of microbial oils that are rich in eicosapentaenoic acid, after detection, eicosapenta The content of alkenoic acid reached 42.6%.

Embodiment 3

[0030] Using yeast as the fermentation strain, follow the steps below in sequence:

[0031] (1) fermentation obtains the microbial fermentation liquid rich in linolenic acid;

[0032] (2) after above-mentioned fermentation broth is centrifuged, obtaining solids content is 24.7% concentrated fermentation broth;

[0033] (3) 150kg of the concentrated fermentation broth was put into a 300L reaction kettle, stirred at a temperature of 45°C, and sheared by a colloid mill for 8h to obtain a mixture after cell lysis.

[0034] (4) adding the above-mentioned mixture after cell lysis to the membrane filtration equipment, circulating filtration, and separating the emulsified oil containing moisture

[0035] (5) add the sodium chloride of 4.5% emulsifiable concentrate weight to the emulsified oil containing moisture, and settle after stirring and mixing, get the supernatant to obtain 7736g of microbial oil rich in linolenic acid, and after detection, the linolenic acid content reaches 76...

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Abstract

The invention provides an extraction method for microbial oil. The extraction method comprises the following steps: (1) collecting microbial fermentation broth prepared after fermentation and containing abundant polyunsaturated fatty acid oil; (2) subjecting the microbial fermentation broth to lysis so as to obtain a cell lysate mixture; (3) separating moisture-containing emulsified oil from the cell lysate mixture by using a membrane filtration device; and (4) breaking the emulsified liquid of oil and water by using a non-solvent de-emulsifier so as to obtain the microbial oil. According to the invention, no solvent is used in the process of extraction, so environmental pollution caused by usage and recovery of solvents is avoided, and production cost is lowered; moreover, since solvents bring colloids like phosphatide in mediums into oil, no usage of solvents is beneficial for reducing colloids in the oil, improving the quality of the oil and lowering consumption in oil refining.

Description

technical field [0001] The present invention relates to a method for extracting microbial oils and fats, in particular to a method for extracting oils and fats containing polyunsaturated fatty acids from microorganisms without using a solvent. Background technique [0002] Polyunsaturated fatty acids refer to straight-chain fatty acids containing two or more double bonds and a carbon chain length of 18 to 22 carbon atoms, mainly including linoleic acid, gamma-linolenic acid, arachidonic acid, eicosanoic acid. Pentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, etc. Polyunsaturated fatty acids, as essential nutrients for infant development, play an important role in the growth process, typical representatives are arachidonic acid and docosahexaenoic acid. In infants and young children, arachidonic acid is an essential fatty acid, and the lack of arachidonic acid may have serious adverse effects on the development of human tissues and organs, especially the devel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B3/00C11B3/02C11B3/04C11B1/00C11B1/02
CPCC11B1/00C11B1/02C11B3/00C11B3/02C11B3/04C11B3/001C11B1/025C11B3/008
Inventor 汪志明李翔宇陆姝欢陈龙周强易德伟
Owner CABIO BIOTECH WUHAN CO LTD
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