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Different gene combinations realizing ectopic expression and application of different gene combinations in increase of oil content in plant nutrient tissue

A technology of gene combination and vegetative tissue, applied in the field of genetic engineering, can solve problems such as tissue distortion, seedling somatic embryogenesis, and normal growth of transgenic plants, and achieve the effect of increasing oil content

Active Publication Date: 2017-12-29
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

Subsequently, Mendoza et al. overexpressed the transcription factor LEAFY COTYLEDON2 (LEC2) involved in the regulation of seed maturation and oil accumulation in Arabidopsis, and found that seed-specific mRNA was accumulated in vegetative tissues, and the storage TAG content increased, but At the same time, somatic embryogenesis occurred in the seedlings, and the tissues were distorted and deformed, which affected the normal growth of transgenic plants

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  • Different gene combinations realizing ectopic expression and application of different gene combinations in increase of oil content in plant nutrient tissue
  • Different gene combinations realizing ectopic expression and application of different gene combinations in increase of oil content in plant nutrient tissue
  • Different gene combinations realizing ectopic expression and application of different gene combinations in increase of oil content in plant nutrient tissue

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Embodiment

[0058] 1. Construction of transcription unit and screening of target gene

[0059] In order to construct different plant transcription units, the applicant selected 35S and BCH1, which can be constitutively expressed in vegetative organs, as promoters. In addition, the target genes involved in oil synthesis were also selected: (i) the gene GAT1 encoding 3-phosphoglycerol acyltransferase in yeast; (ii) a gene DGAT1 encoding diacylglycerol acyltransferase, the former and the latter were involved in lipid synthesis, respectively. First and third acylation reactions of synthesis; (iii) oleosin 2 (OLEO2) encodes an oil body chimeric protein that maintains the integrity and stability of oil droplets; (iv) converts vegetative cells into embryogenic cells The regulatory factor LEC2, whose ectopic expression in leaves can increase the synthesis and accumulation of leaf oil.

[0060] Among the above four target genes, GAT1 is derived from Saccharomyces cerevisiae, DGAT1, OLEO2 and LEC2...

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Abstract

The invention relates to different gene combinations realizing ectopic expression and an application of the different gene combinations in increase of oil content in plant nutrient tissue, and belongs to the field of genetic engineering. Four genes, namely, LEC2, DGAT1, OLEO2 and yeast GAT1, involved in the different oil synthesis and accumulation processes respectively are selected to be linked with promoters capable of driving the genes to express in the plant nutrient tissue, and different expression vectors consisting of a single gene or multiple genes are constructed and transferred in the plant tissue, so that the target genes express ectopically or heterogeneously in the plant nutrient tissue; finally, transgenic plants with significantly increased oil content in the nutrient tissues are obtained by screening and are applied to production and application of various substances associated with vegetable oil, for example, increase of nutrients of animal feed and promotion production of biofuel.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to different gene combinations of ectopic expression and its application in increasing the oil content of plant vegetative tissues. Background technique [0002] Vegetable oils are versatile and are not only a major source of cooking oil, but are also used in animal feed, soaps, surfactants, cosmetics, paints, lubricants, and in the production of biodiesel. According to reports, the consumption of edible oil will double before 2030; the chemical industry also hopes that vegetable oil can replace 40% of crude oil in 20 years, so the demand for vegetable oil is increasing sharply. However, due to population growth and reduction of arable land, China will face a severe shortage of edible oil and chemical crude oil. It is hoped that by improving and changing the agronomic and quality traits of oil plants (including seed yield, oil content and fatty acid compositi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/66A01H5/00
CPCC12N15/66C12N15/8247
Inventor 牛蕾蕾雷洁郑志富甘毅陈亚东薛金嫚刘宏波
Owner ZHEJIANG FORESTRY UNIVERSITY
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