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Preparation and quality detection method of amomum para-tsaoko total flavonoids extract

A quality detection method, a technology of total flavonoids, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of non-volatile chemical components, pharmacological activity, safety, and lag in the development and utilization of Brassica fructus , to achieve the effect of reasonable, stable and feasible process conditions, reduced analysis and testing costs, and less residue

Active Publication Date: 2017-12-29
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, except for the volatile oil components ([Title] GC-MS analysis of the volatile oil of the grass fruit [Authors] Qin Lanfang, Huang Yunfeng, Hu Qimin, etc., Guangxi Academy of Traditional Chinese Medicine [Title] Traditional Chinese Medicine Herald, 2014(11): 23- 25. [Title] GC-MS Analysis of the Volatile Oil of Red Grass Fruit and White Grass Fruit in Guangxi [Authors] Huang Yunfeng, Qin Lanfang, Hu Qimin, etc., Guangxi Academy of Traditional Chinese Medicine [Title] Modern Chinese Medicine Research and Practice, 2014(02): 22–24), there are no relevant research reports on a series of issues such as the non-volatile chemical components, pharmacological activity, and safety of Brassica fructus
[0003] Fructus fructus has extensive cultivation and long-term folk use foundation in Guangxi, but there are few studies on its pharmacy at present, and the development and utilization of fructus fructus is relatively lagging behind

Method used

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  • Preparation and quality detection method of amomum para-tsaoko total flavonoids extract
  • Preparation and quality detection method of amomum para-tsaoko total flavonoids extract
  • Preparation and quality detection method of amomum para-tsaoko total flavonoids extract

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Experimental program
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Effect test

Embodiment 1

[0048] Grind the dried fruit of Brassica fructus into 200 g of coarse powder, add 10 times the weight of methanol with a volume concentration of 70% for extraction, heat and reflux in a water bath at 90°C for 3 times, each time for 1.5 hours, filter, and combine the filtrates, Recover methanol or ethanol from the filtrate and concentrate under reduced pressure without alcohol smell, add water to make up to 5000ml (concentration 0.04g crude drug amount / ml), filter through four layers of gauze until the clear liquid, filter, pass through HP-20 macroporous adsorption resin column (upper The volume of the sample liquid is 10 times the volume of the filled macroporous adsorption resin), and the flow rate of the upper column is 2 BV / h; wash with 10 times of water, and discard the washing solution to fill 15 times the volume of the macroporous adsorbent resin with a volume concentration of 30%. ethanol elution, the elution flow rate is 1BV / h; collect the eluent, concentrate the eluent...

Embodiment 2

[0050] Take the dried fruit of Brassica fructus and crush it into 500g of coarse powder, add 15 times the amount of methanol with a volume concentration of 80% according to the weight for extraction, heat and reflux extraction in a water bath at 80°C for 4 times, each time for 1 hour, filter, combine the filtrate, the filtrate Recover methanol or ethanol and concentrate under reduced pressure without alcohol smell, add water to make up to 9000 ml (0.056g medicinal materials / ml), filter through four layers of gauze until the clear liquid is filtered, and pass through HP-20 macroporous adsorption resin column (loading solution The volume is 20 times the volume of the resin), and the flow rate on the column is 2BV / h; wash with 10 times of water, discard the washing solution, and elute with ethanol with a volume concentration of 90% and 10 times the volume of the macroporous adsorbent resin, and collect the washed Ethanol was removed, and the elution flow rate was 3 BV / h; ethanol w...

Embodiment 3

[0052] Take the dried fruit of Brassica fructus and crush it into 500 g of coarse powder, add 5 times the amount of 100% methanol according to the weight to extract, heat and reflux in a water bath at 80°C for 3 times, each time for 0.5 hours, filter, combine the filtrate, and recover methanol from the filtrate Or ethanol and concentrated under reduced pressure without alcohol smell, add water to make up to 7500 ml (0.067 g medicinal material / ml), filter through four layers of gauze to a clear liquid, filter, pass through AB-8 macroporous adsorption resin column (the volume of the sample solution is 10 times the volume of the resin), and the column flow rate is 4 BV / h; wash with 10 times of water, discard the washing liquid, and elute with 15 times the volume of the macroporous adsorption resin, and the volume concentration is 70% ethanol, and the elution flow rate is 3BV / h; collect the eluent, concentrate the eluent to the extract under reduced pressure, and dry it in vacuum a...

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Abstract

The invention relates to a preparation and quality detection method of traditional Chinese medicine extract, in particular to a preparation and quality detection method of amomum para-tsaoko total flavonoids extract. The method comprises the following steps: crushing and alcohol extracting dry fruits of amomum para-tsaoko, performing reflux extraction with methanol or ethanol, concentrating, adding water, mixing, suspending, eluting by using a macroporous adsorption resin column chromatograph (ethanol-water system), concentrating, vacuum drying, and obtaining the amomum para-tsaoko total flavonoids extract. The preparation method of the amomum para-tsaoko total flavonoids extract is reasonable, stable, short in production period, and suitable for industrialized production. The invention adopts an ultraviolet-visible light spectrophotometry, a high-performance liquid-phase chromatographic one-measuring multi-evaluation method and an area normalization method to determine the content of amomum para-tsaoko total flavonoids in the amomum para-tsaoko total flavonoids extract. The method is accurate, stable, and simple in operation. The amomum para-tsaoko total flavonoids extract prepared by the invention has pharmacologic actions such as inflammation resistance, bacterial resistance and the like.

Description

technical field [0001] The invention relates to a preparation and quality detection method of an extract of traditional Chinese medicine, in particular to a preparation and quality detection method of a total flavonoids extract of Brassica fructus. Background technique [0002] Tsaoguo, also known as Baicaoguo and Guangxi Tsaoguo, is a plant of the genus Cardamom in the ginger family ( Amomum paratsao-ko S.Q.Tong et Y.M.Xia) are cultivated on a large scale in Guangxi and other regions. It is used as a substitute for grass fruit in Guangxi Zhuang people for Titian or medicinal purposes. It is clinically used to treat abdominal fullness and cold pain, nausea and vomiting, food accumulation, phlegm, malaria and other diseases. However, so far, except for the volatile oil components ([Title] GC-MS analysis of the volatile oil of the grass fruit [Authors] Qin Lanfang, Huang Yunfeng, Hu Qimin, etc., Guangxi Academy of Traditional Chinese Medicine [Title] Traditional Chinese Medic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/38
CPCG01N30/02G01N30/38G01N2030/388
Inventor 柴玲刘布鸣林霄陈明生黄艳冯军
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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