Upconversion-based luminous marker, protein chip and detection method

A technology of luminescent markers and protein chips, which is applied in the field of protein detection, can solve the problems of easy bleaching, low signal-to-noise ratio, and inactivation of biologically active molecules of biomolecules, so as to reduce background noise, improve signal-to-noise ratio, and reduce photobleaching Effect

Pending Publication Date: 2017-12-29
SUZHOU YOUHAN INFORMATION TECH CO LTD
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the fluorescent markers commonly used in protein chip technology are single-photon fluorescent dyes, such as Cy3, Cy5, alexa488, etc. These markers have the characteristics of single-photon excitation, easy bleaching, and low signal-to-noise ratio. The inevitable result of the high false positive rate and fals

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Upconversion-based luminous marker, protein chip and detection method
  • Upconversion-based luminous marker, protein chip and detection method
  • Upconversion-based luminous marker, protein chip and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The biologically active molecules of the present invention may be selected from antigens, antibodies, polynucleotides, drugs, lectins, cells.

[0044] As an example of the present invention, we use the fluoride NaYF 4 As a substrate, Yb 3+ As a sensitizer (accounting for about 20% in the system), it can be flexibly matched with Er 3+ ,Tm 3+ and Ho 3+ and other activators (the total amount accounts for about 2% in the system), so as to reduce the energy loss in the cross-relaxation and improve the up-conversion luminous efficiency. Through the flexible matching of activators, various emission spectra can be obtained, which can be flexibly applied to the fluorescent labeling of protein chips. The UCNPs used in this technique are all synthesized by our laboratory.

[0045] Since the synthesized UCNP is an oily particle, and the antigen to be tested in the sample to be tested is generally water-soluble, UCNP cannot be directly used for fluorescent labeling, and a serie...

Embodiment 2

[0061] As an example of the present invention, the above system can be used to quantitatively detect the concentration of morphine in urine.

[0062] The pretreatment solution contains UCNP-morphine monoclonal antibody and UCNP-goat IgG, wherein UCNP-goat IgG is a quality control substance with known concentration. The detection site morphine-bovine serum albumin cross-linked antigen and the quality inspection site rabbit anti-goat IgG are immobilized on the protein chip.

[0063] The detection process is as follows:

[0064] 1) Mix the sample to be tested and the pretreatment solution evenly. At this time, the morphine antigen in the sample to be tested is specifically combined with the UCNP-morphine monoclonal antibody in the pretreatment solution to form a tight immune complex UCNP-morphine monoclonal antibody-morphine antigen. That is, there are three kinds of particles in the liquid at the same time, the immune complex UCNP-morphine monoclonal antibody-morphine antigen,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an up-conversion luminescent marker based on an up-conversion luminescent marker, a protein chip and a detection method. The array of biologically active molecules on a glass slide or other carrier medium has a detection antibody fixed at the detection site, which can detect It specifically binds to the target antigen of the immune complex, and the quality inspection antibody is immobilized on the quality inspection site, which will specifically bind to the antigen of the quality inspection substance in the pretreatment solution; the excitation device of the line scan is a near-infrared light source. The dichroic mirror is irradiated on the protein chip, and the detection device of the line scanning technology is a line array camera, which simultaneously collects the visible light intensity signals emitted by each point on the line. The UCNP of the present invention cross-links bioactive molecules in a covalent manner, which improves the reliability and stability of the system on the premise of ensuring the detection sensitivity.

Description

technical field [0001] The invention belongs to the field of protein detection, and relates to an up-conversion luminescent marker, a protein chip and a detection method, in particular to a protein chip that uses an up-conversion luminescent nanomaterial, UCNP, as a fluorescent marker, and uses a line light source to excite and a line scanner to collect The technical method of signaling. Background technique [0002] Upconversion refers to the process of converting two or more photons into one high-energy photon, that is, converting near-infrared radiation into visible light. Up-conversion materials are generally composite materials composed of a crystalline matrix and a low-concentration rare earth element dopant. When preparing novel upconversion nanoparticles (UCNPs) with excellent optical properties, suitable hosts and dopants are particularly important. Although theoretically, upconversion can be obtained from most rare-earth element-doped crystalline materials, effic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68G01N33/533
CPCG01N33/533G01N33/68
Inventor 何赛灵
Owner SUZHOU YOUHAN INFORMATION TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap