Upconversion-based luminous marker, protein chip and detection method

Abstract

The invention discloses an upconversion-based luminous marker, a protein chip and a detection method. An antibody A is immobilized at a detection site of a bioactive molecule array on a glass slide or other carrying medium and can be specifically bound with an immune complex target antigen, and a quality testing antibody is immobilized at a quality testing site of the bioactive molecule array and can be specifically bound with a quality testing substance antigen in a pretreatment solution; an excitation device for line scanning is a near infrared light source which irradiates the protein chip through a dichroscope, a detection device used in a linear scanning technology is a line array camera, and the line array camera collects visible light intensity signals sent from each point on the line. UCNPs (upconversion nanoparticles) are crosslinked with bioactive molecules in a covalence manner, and the reliability and the stability of a system are improved on the premise that the detection sensitivity is guaranteed.

Description

technical field [0001] The invention belongs to the field of protein detection, and relates to an up-conversion luminescent marker, a protein chip and a detection method, in particular to a protein chip that uses an up-conversion luminescent nanomaterial, UCNP, as a fluorescent marker, and uses a line light source to excite and a line scanner to collect The technical method of signaling. Background technique [0002] Upconversion refers to the process of converting two or more photons into one high-energy photon, that is, converting near-infrared radiation into visible light. Up-conversion materials are generally composite materials composed of a crystalline matrix and a low-concentration rare earth element dopant. When preparing novel upconversion nanoparticles (UCNPs) with excellent optical properties, suitable hosts and dopants are particularly important. Although theoretically, upconversion can be obtained from most rare-earth element-doped crystalline materials, effic...

AI Technical Summary

Problems solved by technology

[0006] At present, the fluorescent markers commonly used in protein chip technology are single-photon fluorescent dyes, such as Cy3, Cy5, alexa488, etc. These markers have the characteristics of single-photon excitation, easy bleaching, and low signal-to-noise ratio. The inevitable result of the high false positive rate and false negative rate of the protein chip of the marker

In addition, due to the high energy of the excitation light, the biodetection will inevitably cause the autofluorescence of biomolecules, and even cause the inactivation of bioactive molecules.

These defects greatly limit the scope of application of protein chips in biological assays

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