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Fermented feed production process capable of increasing content of acid soluble proteins

A production process and technology of fermented feed, applied in the field of fermented feed production technology, can solve the problems of reducing anti-nutritional factors, producing acid, unable to effectively increase the content of acid-soluble protein in the feed, etc. low cost effect

Inactive Publication Date: 2018-01-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In today's fermented feed industry, in the screening of strains used for fermentation, often due to the clear purpose of strain screening, the selected strains have powerful special functions such as reducing anti-nutritional factors and producing acid, but cannot effectively increase the acid-soluble protein in the feed. Content, so it is necessary to use other methods as supplements to increase the acid-soluble protein content of the feed

Method used

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  • Fermented feed production process capable of increasing content of acid soluble proteins
  • Fermented feed production process capable of increasing content of acid soluble proteins

Examples

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Effect test

Embodiment 1

[0032] A fermented feed production process for increasing acid-soluble protein content, the specific steps are as follows:

[0033] (1) Preparation of mixed strain seed liquid: culture yeast and lactic acid bacteria separately to obtain respective strain liquids, and then mix to obtain mixed strain seed liquid;

[0034] (2) Pretreatment of concentrated feed: sterilize the concentrated feed at 90°C for 10 minutes, and then adjust its moisture content between 45% and 55%;

[0035] (3) Mixed fermentation: Mix the seed solution of mixed strains obtained in step (1) with the pretreated concentrate feed of step (2), then add neutral protease with enzyme activity ≥ 50000U / g, mix and ferment for 48 hours, and the fermentation temperature 32°C; the mass ratio of mixed strain seed solution, neutral protease and concentrated feed is 1:0.009:8.

[0036] (4) Drying and crushing: drying the fermented concentrate feed in step (3), cooling to room temperature, and crushing to obtain fermente...

Embodiment 2

[0041] A fermented feed production process for increasing acid-soluble protein content, the specific steps are as follows:

[0042] (1) Preparation of mixed strain seed liquid: culture yeast and lactic acid bacteria separately to obtain respective fermentation liquids, and then mix to obtain mixed strain seed liquid;

[0043] (2) Pretreatment of concentrated feed: sterilize the concentrated feed at 100°C for 20 minutes, then adjust its moisture content between 45% and 55%;

[0044] (3) Mixed fermentation: Mix the seed solution of mixed strains obtained in step (1) with the pretreated concentrate feed of step (2), then add neutral protease with enzyme activity ≥ 50000U / g, mix and ferment for 72 hours, and the fermentation temperature 37°C; the mass ratio of mixed strain seed solution, neutral protease and concentrated feed is 1:0.011:12.

[0045] (4) Drying and crushing: drying the fermented concentrate feed in step (3), cooling to room temperature, and crushing to obtain ferm...

Embodiment 3

[0050] A fermented feed production process for increasing acid-soluble protein content, the specific steps are as follows:

[0051] (1) Preparation of mixed strain seed liquid: ferment yeast and lactic acid bacteria separately to obtain respective fermentation liquids, and then mix to obtain mixed strain seed liquid;

[0052] (2) Pretreatment of concentrated feed: sterilize the concentrated feed at 95°C for 15 minutes, and then adjust its moisture content between 45% and 55%;

[0053] (3) Mixed fermentation: Mix the seed solution of mixed strains obtained in step (1) with the pretreated concentrate feed of step (2), then add neutral protease with enzyme activity ≥ 50000U / g, mix and ferment for 60 hours, and the fermentation temperature 35°C; the mass ratio of mixed strain seed solution, neutral protease and concentrated feed is 1:0.01:10.

[0054] (4) Drying and crushing: drying the fermented concentrate feed in step (3), cooling to room temperature, and crushing to obtain fe...

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Abstract

The invention discloses a fermented feed production process capable of increasing the content of acid soluble proteins. The production process specifically comprises the following steps: (1) preparinga mixed strain seed solution; (2) pre-treating concentrated feed: sterilizing the concentrated feed for 10 to 20 min at 90 to 105 DEG C, and then adjusting the water content to be between 45 percentand 55 percent; (3) mixing and fermenting: uniformly mixing the mixed strain seed solution obtained in step (1) and the concentrated feed pre-treated in step (2), adding neutral protease, and fermenting; and (4) drying and crushing: naturally drying or baking the concentrated feed fermented in step (3), cooling to room temperature, and crushing, thus obtaining the fermented feed. According to theproduction process, saccharomycetes and lactobacillus are made into the mixed strain seed solution to ferment the concentrated feed, and the mixed strain seed solution is synergistically cooperated with the neutral protease, so that anti-nutrition factors in the feed can be degraded, and the content of the acid soluble proteins of the obtained fermented feed can be significantly increased.

Description

technical field [0001] The invention relates to a fermented feed production process, in particular to a fermented feed production process for increasing acid-soluble protein content, and belongs to the technical field of fermented feed. Background technique [0002] The main purpose of fermentation and enzymatic hydrolysis of meal and plant protein is to degrade the macromolecular protein into small molecular protein, even into polypeptide and small peptide, and degrade anti-nutritional factors such as phytic acid, glucosinolate and tannin at the same time, thereby improving Its digestibility in animals and the immunity of the body. The content of small molecule protein is the main index to evaluate the quality of fermented and enzymatically hydrolyzed meal protein. [0003] One of the standards, and the feed industry often uses trichloroacetic acid acid-soluble protein (TCA-N) as a routine evaluation index for the content of small molecular proteins in such products. Stud...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/37
CPCY02P60/87
Inventor 汪海峰毛江笛章亭洲刘建新
Owner ZHEJIANG UNIV
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