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A method for extracting chlorogenic acid from lysimachia christinae Hance

A technology of sagebrush and chlorogenic acid, applied in chemical instruments and methods, separation/purification of carboxylate, preparation of carboxylate, etc., can solve the problems of low solubility, harsh equipment requirements, low selectivity, etc.

Inactive Publication Date: 2018-01-09
桂林纽泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the common solvent C0 2 It has the characteristics of non-polarity and small relative molecular mass, which makes the extraction effect unsatisfactory due to its low solubility and low selectivity for some substances. In actual operation, it is often necessary to increase the extraction rate by adding entraining agents. Finally, it is necessary to try to remove the entraining agent in the volatile oil, which will inevitably lead to the complexity of the process and the loss and oxidation of volatile components; in addition, because water is insoluble in CO 2 , so the supercritical C0 2 The extraction process often needs to pre-dry the raw materials, which increases the cost and causes the loss of aromatic compound oil
In addition, when extracting volatile oils from plants, due to supercritical CO 2 It has similar solubility not only to volatile oils but also to low-polarity compounds such as waxes, fatty acids, colored substances and resins in the stratum corneum. These substances will also be extracted during the extraction process, although complex systems can also be used. Pure volatile oil, but the operation is cumbersome and the requirements for equipment are more stringent

Method used

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  • A method for extracting chlorogenic acid from lysimachia christinae Hance
  • A method for extracting chlorogenic acid from lysimachia christinae Hance

Examples

Experimental program
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Effect test

Embodiment 1

[0023] The method for extracting chlorogenic acid from Desmodium, specifically comprises the following steps:

[0024] 1) Clean the money grass, dry it, pulverize it with a pulverizer, and pass through a 20-mesh sieve;

[0025] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 240-250°C, pressure 8-10Mpa, extraction time 0.5h, extract Desmodium sativa, the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature, and the extract is obtained;

[0026] 3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through DM130 macroporous re...

Embodiment 2

[0028] The method for extracting chlorogenic acid from Desmodium, specifically comprises the following steps:

[0029] 1) Clean the money grass, dry it, pulverize it with a pulverizer, and pass through a 40-mesh sieve;

[0030] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 240-250°C, pressure 8-10Mpa, extraction time 1.0h, extract Desmodium sativa, the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature, and the extract is obtained;

[0031] 3) Purification: Concentrate the extract obtained in step 2), control the temperature to ≤50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, and perform column chromatography through AB-8 macroporo...

Embodiment 3

[0033] The method for extracting chlorogenic acid from Desmodium, specifically comprises the following steps:

[0034] 1) Clean the money grass, dry it, pulverize it with a pulverizer, and pass through an 80-mesh sieve;

[0035] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 240-250°C, pressure 8-10Mpa, extraction time 1.0h, extract Desmodium sativa, the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature, and the extract is obtained;

[0036]3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through D101 macroporous res...

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Abstract

A method for extracting chlorogenic acid from lysimachia christinae Hance is disclosed. The method includes 1) a step of cleaning and airing the lysimachia christinae Hance, crushing the lysimachia christinae Hance by utilizing a crusher, and sieving the crushed lysimachia christinae Hance; 2) a step of performing subcritical water extraction, namely a step of adding the treated raw material intoan extraction kettle, adding hydroxyethyl cellulose the weight of which is 0.001 part of the weight of the raw material into the kettle, injecting deoxygenated deionized water into the kettle, extracting the lysimachia christinae Hance at 240-250 DEG C under 8-10 MPa for 0.5-1.0 h, after the extraction is finished, performing pressure relief, allowing an extraction liquid to flow into a collectiontank, and cooling the extraction liquid to room temperature to obtain the extraction liquid; and 3) a step of purification, namely a step of concentrating the extraction liquid obtained in the step 2), performing column chromatography utilizing macroporous resin, performing elution with an ethanol solution having a concentration of 50% v / v to obtain an eluate, concentrating the eluate, and dryinga concentration product to obtain a finished product.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting chlorogenic acid from Desmodium sativa. Background technique [0002] Chlorogenic acid (Chlorogenic acid) is composed of caffeic acid (Caffeic acid) and quinic acid (Quinic acid) depsipphenolic acid, synonym caffeic tannic acid, chemical name 3-O-caffeoyl quinic acid (3-O -caffeoylquinic acid) molecular formula: C 16 h 18 o 9 , Molecular weight: 354.30, is a benzene compound produced by plants through the shikimic acid pathway during aerobic respiration. Chlorogenic acid has a wide range of biological activities. Modern scientific research on the biological activity of chlorogenic acid has penetrated into many fields such as food, health care, medicine and daily chemical industry. Chlorogenic acid is an important biologically active substance, which has the functions of antibacterial, antiviral, increasing white blood cells, protect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48C07C67/56
Inventor 谢冬养
Owner 桂林纽泰生物科技有限公司
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