Sophora flavescens polysaccharide, its preparation method and liver protection and immune regulation application

A technology of ginseng polysaccharides and uses, applied in the field of Sophora flavescens polysaccharides, can solve problems such as the gap in biological activity of flavescens polysaccharides, less flavescens polysaccharides, etc., and achieve the exact therapeutic effect, reduce inflammation, and inhibit expression

Active Publication Date: 2022-02-01
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Summarizing the above research results, it can be seen that there are few researches on the chemical and biological activities of Sophora flavescens polysaccharides at home and abroad, especially in terms of biological activities, only the capture of free radicals, the inhibition of H22 tumor cell growth and the effect on mouse macrophages have been evaluated in vitro. Promotion of NO secretion by cells
In addition, it can also be seen that there is a large gap in the biological activity of Sophora flavescens polysaccharides obtained by different methods

Method used

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  • Sophora flavescens polysaccharide, its preparation method and liver protection and immune regulation application
  • Sophora flavescens polysaccharide, its preparation method and liver protection and immune regulation application
  • Sophora flavescens polysaccharide, its preparation method and liver protection and immune regulation application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1 Preparation of Sophora flavescens polysaccharide of the present invention (1)

[0061] Step 1: Water Extraction

[0062] Take unextracted Sophora flavescens root and crush it, and pass it through a 20-mesh sieve. Weigh 3 parts of Sophora flavescens root powder, each 1kg. Add 15L of distilled water for extraction at 20°C, 60°C and 100°C respectively. Among them, 24 hours of leaching at 25°C, 5 hours of leaching at 60°C, 1.5 hours of decoction at 100°C, and stirring during the leaching. The extract was filtered, and the filter residues were extracted again under the same conditions. The water extracts extracted twice were combined and centrifuged (3000r / min×20min), and the supernatants obtained at different temperatures were respectively concentrated to 1.5L under reduced pressure at 55°C to obtain concentrated water extracts.

[0063] Step 2: alcohol precipitation

[0064] Add 3 times the volume of ethanol to the concentrated solution extracted at diffe...

Embodiment 2

[0067] Embodiment 2 Preparation of Sophora flavescens polysaccharide of the present invention (two)

[0068] Step 1: Water Extraction

[0069] Take unextracted Sophora flavescens root and crush it, and pass it through a 10-mesh sieve. Weigh the root powder of Sophora flavescens, add distilled water for extraction, the ratio of solid to liquid is 1:20kg / L, the extraction temperature is 0°C, the extraction time is 48 hours, the extraction solution is filtered, and the filter residue is carried out twice under the same conditions. extract. Stirring is performed during the leaching. The extracted water extracts were combined and centrifuged (3000 r / min×20 min), and the obtained supernatant was concentrated to 1.0 L under reduced pressure at 50° C. to obtain a concentrated water extract.

[0070] Step 2: alcohol precipitation

[0071] Add 2 times the volume of ethanol to the extracted concentrated solution to make the final concentration of ethanol 80% (mass concentration of et...

Embodiment 3

[0074] Embodiment 3 preparation of Sophora flavescens polysaccharide of the present invention (three)

[0075] Step 1: Water Extraction

[0076] The roots of Sophora flavescens extracted with ethyl acetate were crushed and passed through a 30-mesh sieve. Weigh the root powder of Sophora flavescens, add deionized water for extraction, the ratio of solid to liquid is 1:2kg / L, the extraction temperature is 90°C, the extraction time is 1 hour, the extraction solution is filtered, and the filter residue is further processed under the same conditions. 2 extractions. Stirring is performed during the leaching. The extracted water extracts were combined and centrifuged (3000 r / min×20 min), and the obtained supernatant was concentrated to 1.5 L under reduced pressure at 55° C. to obtain a concentrated water extract.

[0077] Step 2: alcohol precipitation

[0078] Add 4 times the volume of ethanol to the concentrated solution of extraction to make the final concentration of ethanol 4...

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Abstract

The invention relates to a Sophora flavescens polysaccharide, a preparation method thereof and uses for liver protection and immune regulation. The Sophora flavescens polysaccharide is extracted from the roots of Sophora flavescens by water extraction, and the present invention further separates and obtains the polysaccharide components in the Sophora flavescens polysaccharide. Experiments have confirmed that the polysaccharides of Sophora flavescens and its polysaccharide components can promote the proliferation of mouse spleen cells and the secretion of cytokines, promote the proliferation of human liver cells and reduce the damage of chemical agents to cells, and can inhibit the expression of hepatitis B surface antigen and core antigen. Expression, inhibit the proliferation of liver cancer cells and inhibit the damage caused by ConA to mouse liver and kidney, so it can be used to prepare immune regulation, anti-tumor and anti-liver injury drugs.

Description

technical field [0001] The invention relates to the fields of traditional Chinese medicine, hepatology and immunology, and in particular to a Sophora flavescens polysaccharide, a preparation method thereof and uses for liver protection and immune regulation. Background technique [0002] Sophora flavescens is the dried root of Sophora flavescens Ait., a perennial herb or shrub. Hematochezia, jaundice, anuria, red and white vaginal discharge, vulvar swelling and itching, eczema, eczema, skin itching, mange and leprosy, etc. External treatment of trichomonal vaginitis [2015 edition of the Pharmacopoeia of the People's Republic of China, Part One, P202]. [0003] Modern pharmacological research has found that Sophora flavescens has medicinal activities such as anti-tumor, anti-virus, anti-liver injury and fibrosis, anti-arrhythmia, anti-parasite and immune regulation. At present, the chemical research of Sophora flavescens mainly focuses on the roots, including alkaloids, flav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/16A61P37/02A61P35/00
Inventor 高月求单俊杰周振华杨华高亚婷孙学华赵修南麻浩李曼张鑫
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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