Specific molecular marker of pleurotus eryngii NX2-O strain as well as acquisition method and application of specific molecular marker

Abstract

The present invention relates to a specific molecular marker of Pleurotus eryngii NX2‑0 strain and its obtaining method and application, the sequences of which are shown in SEQ ID NO.4 and SEQ ID NO.5. Obtaining methods include: (1) extracting genomic DNA; (2) carrying out PCR amplification reaction; (3) performing agarose gel electrophoresis and gel-cutting purification on the PCR amplification product; (4) performing enzyme digestion on the gel-cut purified product and detected by agarose gel electrophoresis. Compared with conventional morphological detection, antagonism test and fruiting test, the present invention has the advantages of short detection time, high accuracy and good repeatability, and has the specificity of NX2-0 strain in the collected Pleurotus eryngii strains .

Description

technical field [0001] The invention belongs to the technical field of bacterial strain molecular markers, in particular to a specific molecular marker of Pleurotus eryngii NX2-0 strain, its obtaining method and application. Background technique [0002] Pleurotus eryngii, also known as Pleurotus eryngii, belongs to Basidinmycotina, Hornenomycetes, Homobasidiomycetidae, Agaricales Pleurotaceae in classification. Pleurotaceae), Pleurotus (Pleurotus), is a large-scale toadstool with good quality. liver fungus". Pleurotus eryngii is high in protein, rich in dietary fiber, and has high edible value. It can not only lower blood pressure and blood lipids, but also the polysaccharides it contains can inhibit certain tumors, enhance the body's immunity, and reduce atherosclerosis. The risk of hardening is the health food of the 21st century. [0003] The development of the cultivation and production of Pleurotus eryngii must rely on strains to a large extent, which is the premise...

AI Technical Summary

Problems solved by technology

The breeding of strains can only be harvested by relying on long-term accumulation. However, because Pleurotus eryngii is asexually reproduced, its strain production process is reproducible, making it easy to be counterfeited, resulting in domestic strains with the same name and different names. The phenomenon of foreign bodies is common

This has brought great losses to mushroom farmers and manufacturers, affected their enthusiasm for cultivation and production, and harmed the development of the entire Pleurotus eryngii industry.

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