Method for preparing high polymer by dissolving fermentation bacteria residue in ionic liquid solvent system

Abstract

The invention provides a method for preparing high polymers by dissolving fermentation scum in an ionic liquid solvent system, the method comprising the following steps: (1) dissolving the fermentation slag with an ionic liquid solvent system at normal temperature or under heating conditions to obtain the scum Dissolving solution and optionally undissolved components; (2) mixing the slag solution with regeneration reagent, the high polymer precipitates and / or coagulates from the scum solution to obtain the first high polymer; optionally , the undissolved components were washed and dried to obtain the second high polymer. In the method, the fermented bacteria residue is treated with ionic liquid to prepare a natural high polymer. The high polymer can be used to prepare degradable materials or as a raw material for other purposes, which is beneficial to the further processing and resource utilization of the bacteria residue, and is a valuable resource for bacteria. The resource utilization of slag provides a new way, which is of great significance to realize the high-value utilization of fungal slag.

Description

technical field

[0001] The invention belongs to the technical field of industrial biology, and relates to a method for preparing high polymers by fermenting bacteria residues, in particular to a method for preparing high polymers by dissolving fermentation bacteria residues in an ionic liquid solvent system. Background technique

[0002] Fermentation residue is solid waste produced during the fermentation process. The annual output of this solid waste is very large. Only the production process of antibiotics in the pharmaceutical industry produces about 2 million tons of residue per year. Fermented scum contains rich organic matter, mainly including protein, polysaccharide, chitin, lipid, etc. %, the crude fat content is about 0.5%-7%. As a natural and renewable biomass resource, fermentation residue has the value of resource utilization such as preparation of biodegradable materials.

[0003] The traditional treatment method of fermented bacteria residue is to use it dire...

Examples

Embodiment 1

[0070] A method for preparing high polymers by dissolving fermentation scum in an ionic liquid solvent system, comprising the steps of:

[0071] (1) Take 20 g of wet penicillin fermented bacteria residue, mix it with a single ionic liquid system (1-butyl-3-methylimidazolium chloride salt) at a mass ratio of 1:0.01, and dissolve it at 10°C for 600 minutes to obtain a solution of bacteria residue and undissolved components;

[0072] (2) The anhydrous salt solution and the bacterial residue solution are mixed evenly at a volume ratio of 0.1:1, so that the solute is precipitated to obtain a regenerated polymer; the undissolved components are washed in the order of n-butanol-water-n-butanol to remove residual ions Liquid, after drying at 10°C, a uniform white polymer powder is obtained.

[0073] The extraction rate of the high polymer was 47.9%.

Embodiment 2

[0075] A method for preparing high polymers by dissolving fermentation scum in an ionic liquid solvent system, comprising the steps of:

[0076] (1) Get wet penicillin fermentation slag (such as figure 2 Shown in a) 20g, after washing with 20mL municipal tap water, suction filtration, the bacterial residue that suction filtration obtains (such as figure 2 Shown in b) dried at 80°C to constant weight; the dried bacteria residue was mixed with a single ionic liquid system (1-butyl-3-methylimidazole acetate) at a mass ratio of 1:10, and dissolved at 90°C 720min to get the bacterium residue solution (such as image 3 shown);

[0077] (2) Methanol, water mixed solution and bacterial residue solution are mixed evenly at a volume ratio of 1:1 to precipitate the solute and obtain a regenerated polymer (such as Figure 4 shown).

[0078] The extraction rate of the high polymer was 48.0%.

Embodiment 3

[0080] A method for preparing high polymers by dissolving fermentation scum in an ionic liquid solvent system, comprising the steps of:

[0081] (1) Take 20 g of wet penicillin fermented bacteria residue, wash with 20 mL of anhydrous salt water and then suction filter, and dry the bacteria residue obtained by suction filtration to constant weight at 160 ° C; dry bacteria residue and single ionic liquid system (choline acetate) Mix at a mass ratio of 1:0.5, and dissolve at 90°C for 600 minutes to obtain a solution of fungus residue;

[0082] (2) Methanol and bacterial residue solution are mixed evenly at a volume ratio of 2:1 to precipitate the solute to obtain a regenerated polymer; the undissolved components are washed in the order of n-butanol-water-n-butanol to remove residual ionic liquid , and dried at 30°C to obtain a white polymer powder (such as Figure 6 shown).

[0083] The extraction rate of the high polymer was 48.1%.

technical field

[0001] The invention belongs to the technical field of industrial biology, and relates to a method for preparing high polymers by fermenting bacteria residues, in particular to a method for preparing high polymers by dissolving fermentation bacteria residues in an ionic liquid solvent system. Background technique

[0002] Fermentation residue is solid waste produced during the fermentation process. The annual output of this solid waste is very large. Only the production process of antibiotics in the pharmaceutical industry produces about 2 million tons of residue per year. Fermented scum contains rich organic matter, mainly including protein, polysaccharide, chitin, lipid, etc. %, the crude fat content is about 0.5%-7%. As a natural and renewable biomass resource, fermentation residue has the value of resource utilization such as preparation of biodegradable materials.

[0003] The traditional treatment method of fermented bacteria residue is to use it dire...

Examples

Embodiment 1

[0070] A method for preparing high polymers by dissolving fermentation scum in an ionic liquid solvent system, comprising the steps of:

[0071] (1) Take 20 g of wet penicillin fermented bacteria residue, mix it with a single ionic liquid system (1-butyl-3-methylimidazolium chloride salt) at a mass ratio of 1:0.01, and dissolve it at 10°C for 600 minutes to obtain a solution of bacteria residue and undissolved components;

[0072] (2) The anhydrous salt solution and the bacterial residue solution are mixed evenly at a volume ratio of 0.1:1, so that the solute is precipitated to obtain a regenerated polymer; the undissolved components are washed in the order of n-butanol-water-n-butanol to remove residual ions Liquid, after drying at 10°C, a uniform white polymer powder is obtained.

[0073] The extraction rate of the high polymer was 47.9%.

Embodiment 2

[0075] A method for preparing high polymers by dissolving fermentation scum in an ionic liquid solvent system, comprising the steps of:

[0076] (1) Get wet penicillin fermentation slag (such as figure 2 Shown in a) 20g, after washing with 20mL municipal tap water, suction filtration, the bacterial residue that suction filtration obtains (such as figure 2 Shown in b) dried at 80°C to constant weight; the dried bacteria residue was mixed with a single ionic liquid system (1-butyl-3-methylimidazole acetate) at a mass ratio of 1:10, and dissolved at 90°C 720min to get the bacterium residue solution (such as image 3 shown);

[0077] (2) Methanol, water mixed solution and bacterial residue solution are mixed evenly at a volume ratio of 1:1 to precipitate the solute and obtain a regenerated polymer (such as Figure 4 shown).

[0078] The extraction rate of the high polymer was 48.0%.

Embodiment 3

[0080] A method for preparing high polymers by dissolving fermentation scum in an ionic liquid solvent system, comprising the steps of:

[0081] (1) Take 20 g of wet penicillin fermented bacteria residue, wash with 20 mL of anhydrous salt water and then suction filter, and dry the bacteria residue obtained by suction filtration to constant weight at 160 ° C; dry bacteria residue and single ionic liquid system (choline acetate) Mix at a mass ratio of 1:0.5, and dissolve at 90°C for 600 minutes to obtain a solution of fungus residue;

[0082] (2) Methanol and bacterial residue solution are mixed evenly at a volume ratio of 2:1 to precipitate the solute to obtain a regenerated polymer; the undissolved components are washed in the order of n-butanol-water-n-butanol to remove residual ionic liquid , and dried at 30°C to obtain a white polymer powder (such as Figure 6 shown).

[0083] The extraction rate of the high polymer was 48.1%.

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