Preparation method and application of soluble euglena polysaccharide

A technology of soluble and euglena, applied in the field of biomedicine, can solve the problem of low solubility of euglena, achieve significant anti-inflammatory effect, excellent suspension, and relieve focal spongy edema

Pending Publication Date: 2022-07-08
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to overcome the problem of low solubility of Euglena in non-extreme pH solutions, and provide a preparation method and application of soluble Euglena

Method used

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  • Preparation method and application of soluble euglena polysaccharide
  • Preparation method and application of soluble euglena polysaccharide
  • Preparation method and application of soluble euglena polysaccharide

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preparation example Construction

[0042] A preparation method of soluble Euglena polysaccharide, the method is method 1 or method 2, wherein, the method 1 comprises the following steps:

[0043] (1) Alkali dissolving: use sodium hydroxide solution or potassium hydroxide solution or calcium hydroxide solution or barium hydroxide lye solution of pH>12 to dissolve euglena, and mechanically stir until the solid of euglena disappears;

[0044] (2) Acid cross-linking: add acid solution dropwise, as the pH decreases, gelatinous precipitation appears in the solution, add acid solution dropwise until the pH of the solution is less than or equal to 7;

[0045] (3) Oxidative bond breaking: add oxidant hydrogen peroxide or sodium peroxide or calcium peroxide solution or hypochlorous acid or sodium hypochlorite until the peroxide concentration in the solution is 0.01g / L~0.1g / L or the available chlorine content is 0.1g / L~1g / L, stir until the gelatinous precipitate formed in step (2) in method 1 disappears, so that the glyco...

Embodiment 1

[0063] Take 10 g of Euglena, dissolve with 100 mL of NaOH (pH=12.5) with a concentration of 1 mol / L, stir with a magnetic stirrer to make the Euglena powder disappear and dissolve completely. Then, 5% hydrochloric acid was added dropwise while stirring continued. After the pH was lowered, lumpy and flocculent gels were precipitated, and acid was continued to be added until the pH was 6. Then add 15ml of 30% (mass fraction) hydrogen peroxide to break the bond. At this time, the solution produces a large number of bubbles, supplemented by magnetic stirrer stirring, the gel block disappears in about a few minutes, and the solution becomes clear. The volume of the solution was 120 ml, and 1 mol / L NaOH solution was added dropwise. After the pH was raised to 7, 2 mg of ascorbic acid was added to stop the oxidation of hydrogen peroxide.

[0064] After standing for 24, the solution did not appear to be figure 2 The phenomenon that the non-soluble Euglena polysaccharide precipitates ...

Embodiment 2

[0072] Take 10g of Euglena polysaccharide, dissolve it with 100mL NaOH (pH=12.5) with a concentration of 1mol / L, stir with a magnetic stirrer, the Euglena polysaccharide powder completely disappears and dissolves completely within one minute. Then, 5% hydrochloric acid was added dropwise while stirring was continued. When the pH was lower than 13, gel precipitation occurred, and acid was continued to be added until the pH was 6. Add 100ml of 30% (mass fraction) sodium hypochlorite to make it oxidize and break bonds. At this time, the solution produces a lot of bubbles. Stir with a magnetic stirrer until the gel completely disappears and the solution becomes clear. Add 5% (mass fraction) hydrochloric acid dropwise. , after adjusting the pH to 6, 2 mg of ascorbic acid was added to stop the oxidation of sodium hypochlorite.

[0073] The SBA-50B glucose analyzer was used to measure the monosaccharide concentration in the solution. The measurement results showed that the glucose mo...

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Abstract

The invention discloses a preparation method of soluble euglena polysaccharide, the method is a method I or a method II, the method I comprises the following steps: (1) alkali dissolution; (2) acid crosslinking; (3) oxidizing and breaking bonds; (4) adjusting the pH value; the second method comprises the following steps: (1) high-temperature key breaking; (2) alkali dissolution; and (3) adjusting the pH value to obtain the soluble euglena polysaccharide. The soluble euglena polysaccharide product prepared by the method disclosed by the invention is wide in application range and pollution-free to the environment, and the prepared soluble beta glucan has a good application prospect in the fields of cosmetics and medicines.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a preparation method and application of soluble Euglena polysaccharide. Background technique [0002] Euglena polysaccharide is a linear β-1,3-glucan crystal particle synthesized and accumulated by the intracellular synthesis of Euglena slenderum. This crystal is folded by a triple helix structure formed by linear β-1,3-glucan molecules. formed later. The crystal particles are insoluble in water, insoluble in weak alkali to acidic solutions, but soluble in strong alkali solutions. After dissolving in strong alkalis, colloidal precipitates will be formed once the pH is adjusted to the weak alkali or acid range. The solubility of polysaccharide is related to whether it can be used in the form of injection, skin care lotion, etc. The inability to obtain soluble Euglena polysaccharide that is not affected by pH is the main factor limiting the application of Euglena polysacchari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A61K8/73A61K31/715A61Q19/00
CPCC08B37/0024A61K8/73A61K31/715A61Q19/00
Inventor 吕和鑫张有为贾士儒
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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