Prepared reagent for quickly detecting saccharose in honey and storage method and detection method of prepared reagent

A technology of sucrose and honey, which is applied to the measurement of color/spectral characteristics, material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions. It can solve large errors and low efficiency of sucrose content determination, etc. problems, to achieve the effect of ensuring activity, simplifying cumbersome steps, and avoiding reagent contamination

Active Publication Date: 2018-01-12
HANGZHOU TIANMAI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problems of low efficiency and large error in the determination of sucrose content in existing honey, the present invention provides a prefabricated reagent for rapid detection of sucrose in honey with high sensitivity and strong anti-interference ability

Method used

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  • Prepared reagent for quickly detecting saccharose in honey and storage method and detection method of prepared reagent
  • Prepared reagent for quickly detecting saccharose in honey and storage method and detection method of prepared reagent

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A prefabricated reagent for rapid detection of sucrose in honey, consisting of the following raw materials: 8g of sodium chloride, 0.1g of N,N-diethyl-p-phenylenediamine, 1.6g of phosphate (disodium hydrogen phosphate and sodium dihydrogen phosphate The molar ratio of sucrose is 1:5), 0.1g of sucrose isomerase, 0.1g of glucosyl oligosaccharide oxidase and 0.1g of horseradish peroxidase; based on the total mass of 10g of prefabricated reagents for rapid detection of sucrose in honey, honey The prefabricated reagent for rapid detection of sucrose contains 0.3 U / mg of enzyme activity of sucrose isomerase, 0.7 U / mg of enzyme activity of glucosyl oligosaccharide oxidase, and 0.7 U / mg of enzyme activity of horseradish peroxidase The unit is 1.5 U / mg; divide the above-mentioned prefabricated reagent for the rapid detection of sucrose in honey into 50 mg portions, each portion is sealed and packaged in a 10 mL transparent screw-cap glass test tube, and refrigerated at 4°C in the...

Embodiment 2

[0035] A prefabricated reagent for rapid detection of sucrose in honey, consisting of the following raw materials: 8.29g of sodium chloride, 0.05g of N,N-diethyl-p-phenylenediamine, 1.5g of phosphate (disodium hydrogen phosphate and dihydrogen phosphate The mol ratio of sodium is 17:1), sucrose isomerase 0.05g, glucosyl oligosaccharide oxidase 0.06g and horseradish peroxidase 0.05g; Based on the prefabricated reagent total mass 10g of sucrose rapid detection in honey, The prefabricated reagent for the rapid detection of sucrose in honey contains sucrose isomerase with an enzyme activity unit of 0.65 U / mg, the enzyme activity unit with glucosyl oligosaccharide oxidase with a unit of 0.4 U / mg, and the enzyme with horseradish peroxidase The unit of activity is 0.7U / mg; divide the above-mentioned prefabricated reagent for the rapid detection of sucrose in honey into 50mg portions, each portion is sealed and packaged in a 10mL high-transparency polystyrene plastic bottle, and refrig...

Embodiment 3

[0037]A prefabricated reagent for rapid detection of sucrose in honey, consisting of the following raw materials: 7.49g of sodium chloride, 0.15g of N,N-diethyl-p-phenylenediamine, 2g of phosphate (disodium hydrogen phosphate and sodium dihydrogen phosphate The molar ratio is 1:7), sucrose isomerase 0.12g, glucosyl oligosaccharide oxidase 0.12g and horseradish peroxidase 0.12g; Based on the total mass of 10g of prefabricated reagents for rapid detection of sucrose in honey, honey The prefabricated reagent for rapid detection of sucrose contains 1.0 U / mg of enzyme activity of sucrose isomerase, 1.0 U / mg of enzyme activity of glucosyl oligosaccharide oxidase, and 1.0 U / mg of enzyme activity of horseradish peroxidase The unit is 2.0 U / mg; the above-mentioned prefabricated reagent for the rapid detection of sucrose in honey is divided into 50 mg each, and each portion is sealed and packaged in a 10 mL transparent screw-cap glass test tube, and refrigerated at 6°C in the dark.

[0...

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Abstract

The invention provides a prepared reagent for quickly detecting saccharose in honey and a storage method and a detection method of the prepared reagent. The prepared reagent is prepared from the following raw materials in parts by weight: 75-83 parts of a filler, 0.5-1.5 parts of a color developing agent, 15-20 parts of phosphate, 0.5-1.2 parts of sucrose isomerase, 0.6-1.2 parts of glucosyl oligosaccharide oxidase and 0.5-1.2 parts of horse radish peroxidase. The prepared reagent is convenient to carry, simple and efficient in operation process, high in security and strong in anti-jamming capability, large-scale instrument equipment is not needed, and detection results are visual and visible; the detection sensitivity is high, and by use of a spectrophotometer, the limit of detection canreach 1.0 mg/L; and the detection method is high in recovery rate and excellent in reproducibility.

Description

technical field [0001] The invention relates to the technical field of rapid detection of food safety, in particular to a prefabricated reagent for rapid detection of sucrose in honey with high sensitivity and simple operation, and its storage and detection methods. Background technique [0002] Bees collect nectar, secretions or honeydew from plants, mix them with their own secretions, and then fully brew natural sweet substances. Honey contains a variety of inorganic salts and vitamins, iron, calcium, copper, manganese, potassium, phosphorus and other organic acids and trace elements that are beneficial to human health. It also contains sugars and a variety of amino acids. High health effects and nutritional value, deeply favored by consumers. In recent years, the demand for honey in the domestic and international markets has continued to expand. However, the output of honey is difficult to meet the demand of the market, and due to the different sources of honey, there ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/77G01N21/78G01N21/31
Inventor 陈维强黄光荣吴东兵唐玉龙赵澎涛
Owner HANGZHOU TIANMAI BIO TECH
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