Feed liquid mildew preventive and application thereof
An antifungal agent and feed technology, applied in the field of feed additives, can solve the problems of limited mold control effect, achieve the effects of extending the shelf life of feed, inhibiting the production of mycotoxins, and improving the palatability of feed
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example 1
[0018] Example 1 Three kinds of plant essential oils and the mold inhibition experiment of compound plant essential oils thereof
[0019] Weigh 5.0g of pellet feed, soak it in sterilized pure water for 5min, take it out and put it in a petri dish, then place the petri dish in an incubator at 28°C for 48 hours, after the surface of the pellet feed is covered with mold, wash it with 10mL sterile Elute the mold spores with normal saline, take 1mL of the eluate, place it in 100mL of PDA liquid medium, and incubate at 28°C for 48 hours as a spore suspension; make a PDA medium plate, pour the above spore suspension, and punch for later use; Inject 50 μL of clove essential oil, oregano essential oil, cinnamon essential oil, and a mixture of three plant essential oils (1:1:1) into the wells, and after incubation at 28°C for 48 hours, measure the size of the inhibition zone. Do not repeat 3 times, take the average. The results are shown in Table 1. The diameter of the fungal inhibitio...
example 2
[0022] Example 2 is the anti-mold effect of the feed liquid anti-mold agent with compound plant essential oil as active ingredient
[0023] Clove essential oil, oregano essential oil and cinnamon essential oil are uniformly mixed in a ratio of 1:1:1 to prepare a feed compound liquid antifungal agent. After the feed is granulated, use the post-spraying process and atomize with compressed air to evenly spray the above-mentioned feed liquid antifungal agent on the surface of the pelleted feed. The amount of antifungal agent added to the feed is 600.0mL / T. Feed sample 3.0kg. At the same time, 3.0 kg of feed samples that were not sprayed with plant essential oil were collected after pelleting and cooling. The extreme accelerated test is used to predict the shelf life of the feed, that is, the feed sample is stored under higher temperature and humidity conditions, and the moldy time of the feed is multiplied by 6 to predict the shelf life of the feed under conventional storage cond...
example 3
[0027] Example 3 feeding test
[0028] Select 20 three-way hybrid pigs (Duroc x Landrace x Yorkshire) at 21 days of age with similar body weight (average body weight 5.00kg), and randomly divide them into control group and experimental group, with 10 replicates in each group and 1 pig in each replicate pig. The control group was fed the basal diet, and the experimental group was fed the basal diet sprayed with compound plant essential oil liquid antifungal agent (600ml / T). The basal ration was a corn-soybean meal-based ration, which was prepared according to the nutritional requirement standard of NRC (1998) pigs. The experimental period was 20 days, with free access to food and water, weighing the initial weight and final weight of the piglets, recording the daily feed intake, and calculating the daily gain, daily feed intake, and feed-to-meat ratio. The results are shown in Table 3. Compared with the control group, the final weight, daily gain and daily feed intake increas...
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