A deep dermal filler and its preparation method and application

A technology of deep dermis and filler, applied in the field of medical materials, can solve the problems of fast absorption and low implantation rate, and achieve the effect of promoting cell proliferation and differentiation, improving survival rate, and improving survival state.

Inactive Publication Date: 2020-09-25
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, natural granular fillers on the market mainly include tissue acellular matrix, including skin and autologous fat, which have problems such as fast absorption after implantation, and stem cells are also faced with being degraded and phagocytized after implantation, and the implantation rate For low-level defects, PRP is mostly used as an auxiliary preparation in combination with other cosmetic fillers in cosmetology
The individual application of the above three in terms of beauty has deficiencies

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of a deep dermal filler in this embodiment specifically comprises the following steps:

[0026] S1: The amniotic membrane at delivery was used as raw material, shaken for 6 hours in a mixture containing 0.25% trypsin + 0.05% Triton X-100, decellularized, and then washed with double distilled water for 12 hours to remove Triton X-100 , freeze-dried and preserved the processed amniotic membrane;

[0027] S2: Under liquid nitrogen freezing conditions, grind the acellular amnion obtained above with a high-speed grinder to obtain fine particle fragments with a particle size distribution of 50-200 μm, and use a dose of 6KGy of cobalt 60 for the obtained particle fragments Irradiate and sterilize for 30 minutes to obtain amniotic membrane particles;

[0028] S3: Soak the amniotic membrane particles in sterile water for 24 hours. Under sterile conditions, dissolve each 100 mg of amniotic membrane particles in 1 mL of sterile water. After shaking and mixing...

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Abstract

The invention discloses a preparation method of deep dermis filler and belongs to the technical field of medical materials. The method comprises specific steps as follows: an amnion obtained after delivery is taken as a raw material, subjected to decellularization through trypsin and Triton X-100, ground into granule fragments by a high-speed grinder under the liquid nitrogen refrigeration condition, the granule fragments are subjected to radiation disinfection with cobalt 60, and amnion granules are obtained; the amnion granules are inoculated with umbilical cord mesenchymal stem cells and subjected to joint culture for 8-10 h, a stem cell-amnion granule mixture is obtained, an isolated blood sample is taken, and PRP (platelet rich plasma) is prepared; the PRP is added to the stem cell-amnion granule mixture, and a PRP-stem cell-amnion granule mixture, namely, the deep dermis filler is obtained. The provided deep dermis filler is compound filler integrating dermis granule filling, stem cell nourishing and PRP nutrition, and the mixture can have the beauty effects of moisturizing skin, making skin fine and smooth and reducing wrinkles on users after used for deep filling of dermis.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a deep dermal filler and a preparation method and application thereof. Background technique [0002] Injection-type granular deep dermal filler has a certain diameter (generally not more than 200 μm), which can produce filling support when injected into the deep dermis. Natural and synthetic pellets on the market include autologous fat pellets, Cymetra, Artecoll, and Radiesse, in which autologous fat pellets are extracted fats ground into fine particles; Cymetra is derived from cadaveric skin, decellularized Finally, it is ground into granular form; Artecoll is a microsphere particle with a diameter of 40-60 μm formed by polymethyl methacrylate material; Radiesse is a microsphere particle with a diameter of 40 μm made of hydroxyapatite. [0003] So far, the natural granular fillers on the market mainly include tissue acellular matrix, including skin and aut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/60A61L27/54A61L27/50
Inventor 王文加赵磊秦子媛
Owner JILIN UNIV
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