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Preparation method and fluorescence detection method for magnetic fluorescent nanometer material

A fluorescent nanomaterial and magnetic technology, applied in the field of nanocomposite material preparation, achieves high stability, cumbersome operation steps, and long detection time

Active Publication Date: 2018-01-19
HENAN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention proposes a preparation of a magnetic fluorescent nano-material and a multicolor fluorescence detection method for a marker of Bacillus anthracis aiming at the defect in the wide application of the prior art for detecting Bacillus anthracis

Method used

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  • Preparation method and fluorescence detection method for magnetic fluorescent nanometer material
  • Preparation method and fluorescence detection method for magnetic fluorescent nanometer material

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preparation example Construction

[0020] The specific steps of a method for preparing magnetic fluorescent nanomaterials are as follows:

[0021] Step one, magnetic Fe 3 O 4 Preparation: take FeCl 3 Dissolve in an appropriate amount of ethylene glycol, disperse to form a clear solution, then add sodium acetate and polyethyleneimine, stir and disperse, put it in a 100mL stainless steel reactor, and react at 200-220℃ for 8-12h. After washing with distilled water and drying at 60 ℃, the surface organically modified magnetic Fe 3 O 4 Nanoparticle

[0022] Step two, magnetic fluorescent material Fe 3 O 4 @CePO 4 : Preparation of Tb-PEG: Dissolve the product obtained in step one in a proper amount of distilled water, and after ultrasonic dispersion, take a certain amount of Ce(NO 3 ) 3 ×6H 2 O solution, Tb(NO 3 ) 3 ×6H 2 The O solution was added in three batches; after stirring for 1 hour, polyethylene glycol and Na were added 2 HPO 4 , Adjust the system with 1mol / L NaOH solution and adjust the pH to 6~8, put it into a st...

Embodiment 1

[0029] A method for preparing magnetic fluorescent nanomaterials, the specific steps are as follows:

[0030] Step one, magnetic Fe 3 O 4 Preparation: Weigh 1.5g FeCl 3 Dissolve in 30mL of ethylene glycol, disperse ultrasonically, then add 2.8g of sodium acetate and 0.58g of polyethyleneimine (molecular weight 10000), stir at room temperature for 30min, then put it into a 100mL stainless steel reactor, and react at 210℃ 8h, after cooling to room temperature, remove the supernatant, absorb the product with a magnet and wash with distilled water 3 times, vacuum dry at 60℃ for 1h to obtain magnetic Fe 3 O 4 Nanoparticle

[0031] Step two, magnetic fluorescent material Fe 3 O 4 @CePO 4 : Preparation of Tb-PEG: Take 1gFe 3 O 4 Nanoparticles are dissolved in 30mL of H 2 In O, 43.2mg Ce(NO 3 ) 3 ×6H 2 O (the amount of substance 0.1mmol) and 9.06mg Tb(NO 3 ) 3 ×6H 2 O (the amount of substance is 0.02mmol), after stirring for 1h, add 4mg polyethylene glycol (PEG, molecular weight 6000) and 3...

Embodiment 2

[0034] A method for preparing magnetic fluorescent nanomaterials, the specific steps are as follows:

[0035] Step one, magnetic Fe 3 O 4 Preparation: Weigh 2 g of FeCl 3 Dissolve in 30mL of ethylene glycol, disperse ultrasonically, then add 3.2g of sodium acetate and 0.62g of polyethyleneimine (molecular weight 10000), stir for 30min at room temperature, put into a 100mL stainless steel reactor, and react at 200℃ for 12h After cooling to room temperature, remove the supernatant, absorb the product with a magnet and wash with distilled water 3 times, and vacuum dry at 60℃ for 1h to obtain magnetic Fe 3 O 4 Nanoparticle

[0036] Step two, magnetic fluorescent material Fe 3 O 4 @CePO 4 : Preparation of Tb-PEG: Take 1.5g Fe 3 O 4 Nanoparticles are dissolved in 40mL of H 2 In O, after ultrasonic dispersion, 51.84 mg Ce(NO 3 ) 3 ×6H 2 O (the amount of substance 0.12 mmol) and 22.65mgTb (NO 3 ) 3 ×6H 2 O (the amount of substance is 0.05mmol), add 8mg polyethylene glycol (PEG, molecular we...

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Abstract

The invention relates to a preparation method and a fluorescence detection method for a magnetic fluorescent nanometer material, belonging to the field of preparation of nanometer composite materials.The magnetic fluorescent nanometer particle provided by the invention is prepared through the following step: with ferroferric oxide nanoparticles as a template, covalently grafting a terbium (Tb) compound with green fluorescence and an europium (Eu) compound with red fluorescence onto the surface of ferroferric oxide through a solvothermal method and a conventional surface modification method soas to obtain the magnetic fluorescent nanometer material capable of emitting green fluorescence. The magnetic fluorescent nanometer material can realize rapid multi-color fluorescence detection of aBacillus anthracis marker DPA, wherein the fluorescent material shows green fluorescence in the absence of DPA, while the fluorescence of a system is changed from green to red due to combination of DPA and europium ions when DPA emerges. The magnetic fluorescent nanometer material prepared by using the method provided by the invention has advantages like low detection limit, wide fluorescent visual range and simple preparation process in detection of the Bacillus anthracis marker DPA, can realize enrichment of Bacillus anthracis by virtue of an external magnetic field, and has certain application prospects.

Description

Technical field [0001] The invention belongs to the field of nano composite material preparation, and relates to a preparation method of magnetic fluorescent nano particles grafted with rare earth composite ions with ferroferric oxide as a template, and rapid multicolor fluorescence detection of Bacillus anthracis marker DPA. Background technique [0002] Bacillus anthracis is the causative agent of anthracnose disease and is a gram-positive aerobic bacteria that forms spores. Anthrax is a major disease of herbivores. Contact with spores in the soil and fur can cause infection. It is highly pathogenic and contagious and poses a great threat to human health. DPA (dipicolinic acid, 2,6-pyridine dicarboxylic acid), as an essential component in spores, is often used as a marker for analysis. Therefore, realizing the rapid detection of DPA can realize the early warning of Bacillus anthracis, which is of great significance to the diagnosis of human and herbivore anthracnose and the ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/81G01N21/64G01N33/569H01F1/00H01F41/00
Inventor 贾磊徐君别红彦孙俊岭沈晓科
Owner HENAN POLYTECHNIC UNIV