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A kind of detection method of traditional Chinese medicine composition

A detection method and composition technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of failing to provide simultaneous detection of multiple related substances, and achieve high accuracy and good reproducibility

Active Publication Date: 2020-08-25
SHANGHAI KAIBAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology fails to provide a detection method with good reproducibility, high stability, and easy operation for related substances, and even fails to provide a method for simultaneously detecting multiple related substances under the same conditions

Method used

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  • A kind of detection method of traditional Chinese medicine composition
  • A kind of detection method of traditional Chinese medicine composition
  • A kind of detection method of traditional Chinese medicine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 Chinese medicine composition soft capsule

[0041] Take 375g of cloves and 375g of cinnamon, add 8 times the amount of water, distill in water for 8 hours, collect the volatile oil, refrigerate for 24 hours, remove the water, and make a total of 36ml of volatile oil.

[0042] Mix 36ml of volatile oil with 364ml of soybean oil and set aside. Take 150g of gelatin, 60g of glycerin, 10g of simple syrup, add 150g of deionized water and mix well, heat to 60-70°C, keep warm for 0.5 hours, add 0.15g of methyl p-hydroxybenzoate, 0.075g of ethyl p-hydroxybenzoate, stir , evacuated, and kept at 70°C for 20 minutes to make a capsule material. Take the mixture of volatile oil and soybean oil, use the capsule material to make soft capsules by pressing method, test, pack separately, and obtain 1000 soft capsules.

Embodiment 2

[0043] Embodiment 2 Determination of related substance content

[0044] 1 Instruments and reagents

[0045] Shimadzu Shimadzu GC-2010Plus gas chromatograph, FID detector; electronic balance; Mili-Q pure water instrument; KQ-500DE ultrasonic cleaner.

[0046] Ethyl acetate is analytically pure; α-caryophyllene (93%, batch number: PS151228-02), β-syringene (90%, batch number: PS151228-04), 1-naphthaleneacetic acid (98%, batch number: PS151228-03 ), methyl salicylate (98%, batch number: PS0257-1000), and eugenyl acetate (98%, batch number: PS151228-01) were all purchased from Chengdu Pusi Biotechnology Co., Ltd.; The soft capsule prepared by Example 1.

[0047] 2 Investigation of chromatographic conditions

[0048] 2.1 Preparation of sample solution

[0049] The preparation method of need testing solution: Accurately weigh 0.8g of soft capsule content, put in 50mL volumetric flask, add ethyl acetate to dissolve, dilute to scale, filter with microporous membrane (0.45μm), seal...

Embodiment 3

[0099] Example 3 Determination of 1-naphthaleneacetic acid content

[0100] 1 Instruments and reagents

[0101] Agilent 1260 high performance liquid chromatograph; DAD detector; electronic balance; Mili-Q pure water instrument; KQ-500DE ultrasonic cleaner.

[0102] Ethyl acetate was analytically pure; 1-naphthaleneacetic acid (98%, batch number: PS151228-03) was purchased from Chengdu Pusi Biotechnology Co., Ltd., and the traditional Chinese medicine composition was a soft capsule prepared according to Example 1.

[0103] 2 Investigation of chromatographic conditions

[0104] 2.1 Preparation of sample solution

[0105] Preparation of reference substance solution: Accurately weigh an appropriate amount of 1-naphthaleneacetic acid reference substance, add ethyl acetate to make 0.7mg·mL -1 The reference substance solution was filtered through a microporous membrane (0.45 μm), and sealed for later use.

[0106] Preparation of the test solution: Accurately weigh about 0.8 g of ...

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Abstract

The invention discloses a gas chromatographic detection method of a traditional Chinese medicine composition taking clove and cassia barks as bulk drugs, and belongs to the field of medicine analysis.The gas chromatographic condition comprises: a capillary column is selected as a chromatographic column, a hydrogen flame ionization detector is adopted, nitrogen is taken as a carrying gas, and theheating program includes performing heating from an initial column temperature of 120-150 DEG C to 180-200 DEG C at a speed of 8-12 DEG C / min and maintaining the temperature for 10-15 min. According to the detection method, alpha-caryophyllene and beta-caryophyllene in the traditional Chinese medicine composition can be well separated and detected in the same detection condition. The method is good in reappearance and high in accuracy, is simple and convenient, and is suitable for detecting the content of related substances in the traditional Chinese medicine composition.

Description

technical field [0001] The invention discloses a detection method of a traditional Chinese medicine composition, in particular discloses a gas chromatography detection method of a traditional Chinese medicine composition made of cloves and cinnamon as raw materials, and belongs to the field of drug analysis. Background technique [0002] The invention relates to a traditional Chinese medicine preparation prepared by combining clove and cinnamon according to a certain ratio and then extracting the volatile oil thereof. The main components are eugenol and cinnamon aldehyde. The preparation has the functions of warming and dispelling cold pathogens, promoting qi and relieving pain, and is suitable for abdominal pain in irritable bowel syndrome, aggravated by cold, diarrhea, constipation, abdominal distension, belching, chills, urgency to defecate or feeling of swelling and falling, after defecation Symptoms such as pain reduction, pale tongue, white tongue coating, stringy or t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 张振华李小雪张雯宋庆宏张小利
Owner SHANGHAI KAIBAO PHARMA