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Tripterygium wilfordii extractive, preparation method thereof and application thereof to preparation of eye drops

A technology of Tripterygium wilfordii and its extracts, which is applied in the field of ophthalmic medicine and drug preparation, can solve the problems of tripterygium wilfordii oral administration of large toxic and side effects, single treatment method, etc., achieve the improvement of frontal inflammation and pain, good curative effect, and small adverse reactions Effect

Inactive Publication Date: 2018-01-30
SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to: overcome the existing problems of relatively single treatment means for dry eye disease and relatively large self-defects of Tripterygium wilfordii oral toxicity and side effects, provide a kind of tripterygium wilfordii extract and its preparation method, and use it to prepare drops Eye drops, with the advantages of safety, effectiveness and stability

Method used

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  • Tripterygium wilfordii extractive, preparation method thereof and application thereof to preparation of eye drops
  • Tripterygium wilfordii extractive, preparation method thereof and application thereof to preparation of eye drops
  • Tripterygium wilfordii extractive, preparation method thereof and application thereof to preparation of eye drops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Take the decoction pieces of Tripterygium wilfordii medicinal material, extract 2 times with 8 times of 95% ethanol, each time for 1 hour, filter, combine the filtrate, recover the ethanol under reduced pressure until there is no alcohol smell, continue to concentrate until the relative density is 1.25 (60°C ) thick paste, the extract is absorbed by neutral alumina, dried under reduced pressure, and pulverized to obtain tripterygium wilfordii ethanol extract neutral alumina mixed sample.

[0021] 2. Take the ethanol extract of Tripterygium wilfordii and mix it with neutral alumina, add 10 times the amount of chloroform to extract for 1 hour, filter, take the filtrate, recover the chloroform, and dry to obtain the tripterygium wilfordii extract.

[0022] 3. Get 2.0g of Tripterygium wilfordii extract, add borax 0.6g, boric acid 12g, ethylparaben 0.3g, methylcellulose 10.0g, sodium alginate 10.0g, add Add water for injection to 1000ml, filter through a G2 vertical disso...

Embodiment 2

[0024] 1. Take Tripterygium wilfordii herb decoction pieces, extract once with 12 times of 95% ethanol, 2 hours each time, filter, and recover the ethanol from the filtrate under reduced pressure until it has no alcohol smell, and continue to concentrate until the relative density is 1.30 (60°C) The thick paste and the extract are adsorbed with neutral alumina, dried under reduced pressure, and pulverized to obtain the ethanol extract of Tripterygium wilfordii and mix with neutral alumina.

[0025] 2. Take the ethanol extract of Tripterygium wilfordii and mix it with neutral alumina, add 8 times the amount of chloroform to extract for 1 hour, filter, take the filtrate, recover the chloroform, and dry to obtain the tripterygium wilfordii extract.

[0026] 3. Take Tripterygium wilfordii extract 10.0g and ethylparaben 0.3g and add it to 80ml of propylene glycol to dissolve to obtain solution 1; take 5.8g of disodium hydrogen phosphate, 8.7g of sodium dihydrogen phosphate and 5.0g ...

experiment example 1

[0027] Experimental example 1 Determination of the effective concentration range of Tripterygium wilfordii extract eye drops

[0028] 1. Experimental materials

[0029] 1.1 Coomassie brilliant blue: Nanjing Jiancheng Institute of Bioengineering; batch number: 20121106; specification: 100T / 96 samples.

[0030] 1.2 Complete Freund's adjuvant: specification: 10mL / bottle, batch number: SLBC5083, stored at 2-8°C, SIGMA Chemical Reagent Co., Ltd.

[0031] 1.3 Fluorescein sodium injection, batch number: 120901, specification: 3mL: 0.6g / bottle, properties: brown clear liquid, storage conditions: airtight storage, manufacturer: Guangzhou Baiyunshan Mingxing Pharmaceutical Co., Ltd.

[0032]1.4 Tacrolimus eye drops, product of Fukusaki Factory of Senshou Pharmaceutical Co., Ltd.; batch number: G011; specification: 0.1% (5ml: 5mg (calculated as tacrolimus)).

[0033] 1.5 Disodium hydrogen phosphate: batch number: 20120715, specification: 500g / bottle, manufacturer: Jiangsu Qiangsheng Fu...

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Abstract

The invention discloses a tripterygium wilfordii extractive, preparation method thereof and the application thereof to the preparation of eye drops. The preparation method comprises the following steps: extracting tripterygium wilfordii medical material decoction pieces with 70 to 95 percent ethanol, filtering, combining filtrate, recycling ethanol under reduced pressure till no alcohol taste exists, continuously concentrating the filtrate to form extract with the relative density of 1.15 to 1.35, performing adsorption with neutral aluminum oxide, drying under reduced pressure and crushing, soas to obtain a tripterygium wilfordii extractive neutral aluminum oxide mixed sample; adding trichloromethane to the tripterygium wilfordii extractive neutral aluminum oxide mixed sample to perform extraction, filtering, taking filtrate, recycling the trichloromethane, and drying, so as to obtain the tripterygium wilfordii extractive. The tripterygium wilfordii extractive overcomes the defect ofhigh toxicity when the tripterygium wilfordii extractive is orally taken, is applied to the preparation of eye drops, has the advantages of safety, effectiveness and stability, the eye drops preparedthrough the ripterygium wilfordii extractive have excellent curative effect on xerophthalmia, and the tripterygium wilfordii extractive has a good application prospect and market value.

Description

technical field [0001] The invention belongs to the technical field of ophthalmic medicine and medicine preparation, more specifically, the invention relates to a tripterygium wilfordii extract, a preparation method thereof and an application in preparation of eye drops. Background technique [0002] With the improvement of human living standards and technological levels, especially the rapid development of electronic and digital products, the average frequency of people's eye use has increased sharply. Coupled with environmental and individual factors, the prevalence of dry eye disease is increasing. It has seriously affected the quality of life of some people. Due to the increase in the number of patients, drugs for the treatment of dry eye syndrome have gradually become a hot spot in the research and development of ophthalmic drugs. [0003] In a normal human body, since the surface of the eye is covered with enough tears, the composition of the tear film is normal, coup...

Claims

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Application Information

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IPC IPC(8): A61K36/37A61K9/08A61P27/02
Inventor 肖百全周文良杨威
Owner SUN YAT SEN UNIV
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