Method for improving purity of vancomycin hydrochloride through ion exchange resin

The technology of vancomycin hydrochloride and ion exchange resin is applied in the field of improving the purity of vancomycin hydrochloride by using ion exchange resin, which can solve the problems of difficult large-scale application, complicated process and low purity, and achieves large processing capacity and simple production process. , the effect of the simple regeneration method

Active Publication Date: 2018-01-30
华北制药华胜有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for improving the purity of vancomycin hydrochloride by using ion exchange resins, so as to solve the problems of complex technology, low purity and difficulty in large-scale application of the existing methods

Method used

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  • Method for improving purity of vancomycin hydrochloride through ion exchange resin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The selected macroporous strongly acidic cation exchange resin is D001, and the volume of the resin is 100cm 3 , first ensure that the resin is converted into a strong acid salt, and then pass the filtered vancomycin fermentation broth into the resin tank for adsorption. The vancomycin content in the fermentation broth is 3.2mg / mL. When the export unit of the resin tank reaches more than 90% of the import unit, it is determined that the main tank is saturated. When the adsorption is saturated, the resin is washed with deionized water until the outlet water sample is clear, and then backwashed until the transmittance of the resin outlet water sample is 68%. Prepare an ammonia solution with a pH of 8.5 in the alkali cleaning circulation tank, and pass it through the resin column for circular washing. When the pH of the solution in the circulating tank is 5.6, backwash with deionized water until the water sample transmittance at the resin outlet 94%. Then use 3 mol / L amm...

Embodiment 2

[0022] The selected macroporous strongly acidic cation exchange resin is D002, and the resin volume is 100cm 3 , first ensure that the resin is converted into a strong acid salt, and then pass the filtered vancomycin fermentation broth into the resin tank for adsorption. The vancomycin content in the fermentation broth is 3.2mg / mL. When the export unit of the resin tank reaches more than 90% of the import unit, it is determined that the main tank is saturated. When the adsorption is saturated, the resin is positively washed with deionized water until the outlet water sample is clear, and then backwashed until the light transmittance of the resin outlet water sample is 65%. Prepare an ammonia solution with a pH of 9.0 in the alkali cleaning circulation tank, and pass it through the resin column for circular washing. When the pH of the solution in the circulating tank is 5.8, backwash with deionized water until the water sample at the resin outlet is transparent. 96%. Then use...

Embodiment 3

[0024] The selected macroporous strongly acidic cation exchange resin is 732, and the resin volume is 100cm 3 , first ensure that the resin is converted into a strong acid salt, and then pass the filtered vancomycin fermentation broth into the resin tank for adsorption. The vancomycin content in the fermentation broth is 3.2mg / mL. When the export unit of the resin tank reaches more than 90% of the import unit, it is determined that the main tank is saturated. When the adsorption is saturated, the resin is positively washed with deionized water until the outlet water sample is clear, and then backwashed until the light transmittance of the resin outlet water sample is 64%. Prepare an ammonia solution with a pH of 9.5 in the alkali cleaning circulation tank, and pass it through the resin column for circular washing. When the pH of the solution in the circulating tank is 6.0, backwash with deionized water until the water sample at the resin outlet is transparent. 97%. Then use ...

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Abstract

The invention provides a method for improving the purity of vancomycin hydrochloride through ion exchange resin. The method comprises the following steps: (a), filtering and then adsorbing a vancomycin fermentation liquor through macroporous strong acid cation exchange resin; (b), washing saturated resin through deionized water after the resin is saturated; (c), circularly washing the saturated resin through alkaline aqueous solution after step (b) is accomplished; (d), eluting the saturated resin through an eluent, so as to form vancomycin eluant; (e), performing concentration and desalination on the eluant through a nanofiltration membrane, and obtaining a vancomycin crude product after water washing. The method ensures that quality indexes, such as the purity, transparency and color degree, of a vancomycin intermediate are greatly improved through the specific macroporous strong acid cation exchange resin, a special washing technology and a membrane system, is simple in production technology, high in yield, easy to control, and suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of antibiotics, and in particular relates to a method for improving the purity of vancomycin hydrochloride by using an ion exchange resin. Background technique [0002] Vancomycin is a glycopeptide antibiotic from Streptomyces orientalis or Agrothrichum, which has a unique triple antibacterial mechanism: inhibiting the protein synthesis of bacterial cell walls, changing the permeability of bacterial cell membranes, and hindering the synthesis of bacterial RNA. This product is approved by the US drug (FDA) for infections caused by meticillin-resistant Staphylococcus (MRSA MRCON) and Enterococcus, and is currently the preferred antibiotic for the treatment of severe infections caused by MRSA MRCON and Enterococci. [0003] The separation and purification methods of vancomycin mainly include chromatographic preparation method, precipitation method, affinity adsorption method, reve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K9/00C07K1/18
Inventor 李宁张强王红霞刘菲谷旭林程曜峰张荣魁张玮
Owner 华北制药华胜有限公司
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