Daubing type microbial powder, preparation method and application thereof in control of tree diseases and insect pests
A technology of microbial powder and microbial agent, applied in botany equipment and methods, fertilizers made from biological waste, application, etc., can solve problems such as unsatisfactory use effect and unsafe pesticides, so as to facilitate growth and protect the external environment Good resistance, good bactericidal and insecticidal effect, good use effect
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Embodiment 1
[0024] According to the corresponding mass percentage, take 20% of the original drug of microbial bacterial agent, 5% of glucose, 0.1% of glutamic acid, 3% of soybean meal powder, 5% of lignosulfonate, 0.5% of magnesium sulfate, 0.5% of manganese sulfate, oxychloride 0.5% iron, 15% modified starch, 1% sodium carboxymethyl cellulose, 1% kudzu root, 2% honeysuckle, 5% white carbon black, the rest is made up with kaolin, put each raw material into a mixing tank and stir for 1-2 hours Make it fully mixed evenly, and process the mixed material by airflow pulverization so that the particle size of the finished product is less than 40 μm, and then the smearable microbial powder is obtained.
[0025] The preparation of the original drug of the above-mentioned microbial bacterial agent is as follows: Bacillus subtilis is inoculated into the shake flask of LB liquid medium, shaken and cultured to the bacterial density OD 600 The value is 0.6-0.8; the LB liquid medium is tryptone 10g / L, ...
Embodiment 2
[0027] According to the corresponding mass percentage, take 20% of the original drug of microbial bacterial agent, 5% of glucose, 0.1% of glutamic acid, 3% of soybean meal powder, 5% of lignosulfonate, 0.5% of magnesium sulfate, 0.5% of manganese sulfate, oxychloride 0.5% iron, 15% modified starch, 1% sodium carboxymethyl cellulose, 1% green tea, 1% Scutellaria chinensis, 1% clove, 5% white carbon black, the rest is made up with kaolin, and the raw materials are put into stirring The tank is stirred for 1-2 hours to make it fully mixed evenly, and the mixture is processed by jet milling to make the particle size of the finished product is less than 40 μm, and the smear-type microbial powder is obtained.
[0028] The preparation of the original drug of the above-mentioned microbial bacterial agent is as follows: inoculate the methylotrophic Bacillus bacillus into the shake flask of LB liquid medium, shake and cultivate to the bacterial density OD 600 The value is 0.6-0.8; the L...
Embodiment 3
[0030] Weigh 20% of the original microbial bacterial agent, 5% of glucose, 0.05% of glutamic acid, 0.05% of valine, 3% of soybean meal powder, 5% of lignosulfonate, 0.5% of magnesium sulfate, and manganese sulfate according to the corresponding mass percentage 0.5%, ferrous chloride 0.5%, modified starch 15%, sodium carboxymethyl cellulose 1%, kudzu root 1%, aloe 2%, white carbon black 5%, the balance is made up with kaolin, put each raw material into The mixing tank is stirred for 1-2 hours to make it fully mixed evenly, and the mixed material is processed by airflow pulverization to make the particle size of the finished product less than 40 μm, and then the smear-type microbial powder is obtained.
[0031] The preparation of the original drug of the above-mentioned microbial bacterial agent is as follows: inoculate Bacillus thuringiensis into LB liquid medium shake flasks, shake and culture until the bacterial density is OD 600The value is 0.6-0.8; the LB liquid medium is t...
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