Adenosine deaminase measuring kit

A technology of adenosine deaminase and detection kit, which is applied to the determination/inspection of microorganisms, measuring devices, instruments, etc., which can solve the problems of poor stability, reduced practicability, reduced ADA activity, and poor stability, so as to increase practicability , the effect of increased sensitivity and improved stability

Inactive Publication Date: 2018-02-02
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The sensitivity of detecting ADA is insufficient, and the content of ADA in serum is relatively small. According to literature reports, the serum ADA reference value of normal people is mostly between 4-20U / L, and the ADA activity in the serum of cancer patients and iron-deficiency anemia patients is significantly reduced. Therefore, the samples Accurate quantification of ADA activity at low and medium values ​​cannot be ignored
Existing ADA detection kits are difficult to accurately quantify low-value ADA activity
Although some highly sensitive detection methods such as molecular fluorescence analysis can be used for the detection of trace substances, the application of ADA activity detection methodology has not yet been developed, and it is not clinically practical
[0017] On the other hand, the stability of this kind of kit when stored at 2-8°C after opening is poor, and generally it can only be stored stably for 4 weeks. The main reason is that 4-aminoantipyrine is easily oxidized, which makes the color develop Decreased ability; there are many kinds of enzymes in the kit, and the stability of the enzymes is easily affected
The poor stability of this type of kit reduces its clinical practicability

Method used

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  • Adenosine deaminase measuring kit
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  • Adenosine deaminase measuring kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Reagent R1: pH7.4

[0052] Tris 6.057g

[0053] Sodium azide 1g

[0054] Peroxidase 600U

[0055] 4-Aminoantipyrine 2mmol

[0056] Purine Nucleoside Phosphorylase 100U

[0057] Xanthine Oxidase 200U

[0058] Reagent R2: pH7.4

[0059] Tris 6.057g

[0060] Sodium azide 1g

[0061] Adenosine 10mmol

[0062] N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline 2mmol

[0063] Calibrator:

[0064] Adenosine deaminase freeze-dried product, dissolved in normal saline before use, diluted to final concentrations of 20U / L, 40U / L, 80U / L, 160U / L, 240U / L, 320U / L,

Embodiment 2

[0066] Reagent R1: pH7.4

[0067] Tris 6.057g

[0068] Sodium azide 1g

[0069] Peroxidase 600U

[0070] 4-Aminoantipyrine 2mmol

[0071] Purine Nucleoside Phosphorylase 100U

[0072] Xanthine Oxidase 200U

[0073] Reagent R2: pH7.4

[0074] Tris 6.057g

[0075] Sodium azide 1g

[0076] Adenosine 10mmol

[0077] N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline 2mmol

[0078] Triton X-100 0.2%

[0079] Span-60 0.2%

[0080] Calibrator:

[0081] Adenosine deaminase freeze-dried product, dissolved in normal saline before use, diluted to final concentrations of 20U / L, 40U / L, 80U / L, 160U / L, 240U / L, 320U / L,

Embodiment 3

[0083] Reagent R1: pH7.4

[0084] Tris 6.057g

[0085] Sodium azide 1g

[0086] Peroxidase 600U

[0087] 4-Aminoantipyrine 2mmol

[0088] Purine Nucleoside Phosphorylase 100U

[0089] Xanthine Oxidase 200U

[0090] Polyhexamethyleneguanidine 0.5g / l

[0091] Ethylene glycol 6%

[0092] Reagent R2: pH7.4

[0093] Tris 6.057g

[0094] Sodium azide 1g

[0095] Adenosine 10mmol

[0096] N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline 2mmol

[0097] Triton X-100 0.2%

[0098] Span-60 0.2%

[0099] Polyhexamethyleneguanidine 0.5g / l

[0100] Calibrator:

[0101] Adenosine deaminase freeze-dried product, dissolved in normal saline before use, diluted to final concentrations of 20U / L, 40U / L, 80U / L, 160U / L, 240U / L, 320U / L,

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Abstract

The invention belongs to the technical field of medicine and biochemistry, in particular to an adenosine deaminase detection kit. The kit consists of reagent R1 and reagent R2. Reagent R1 includes: biological buffer, preservative, peroxidase, 4-aminoantipyrine, purine nucleoside phosphorylase, xanthine oxidase and water; reagent R2 includes: biological buffer, preservative, adenosine, N‑ethyl‑N‑(2‑hydroxy‑3‑sulfopropyl)‑3‑methylaniline, and water. The adenosine deaminase detection kit adopts a continuous monitoring method for determination, the main wavelength is 550nm, and has the advantages of fast sensitivity, good accuracy, high specificity, good stability and the like.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring adenosine deaminase. Background technique [0002] Adenosine deaminase (adenosine deaminase, ADA) is an important enzyme in purine nucleoside metabolism. Its activity is a sensitive indicator to reflect liver damage, and can be used as one of the routine liver function inspection items. The liver enzyme spectrum combined with ALT or GGT can more comprehensively reflect the enzymatic changes of liver diseases. The determination of ADA activity in serum can be used to: judge acute liver injury and residual disease; assist in the diagnosis of chronic liver disease; help in the diagnosis of liver fibrosis; help in the identification of jaundice. In addition, the lack of ADA activity is related to severe combined immunodeficiency disease (SCID). As an important enzyme in nucleic acid metabolism, ADA deficiency can lead to nucleic acid metabolism...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/34
CPCC12Q1/34G01N2333/978
Inventor 陈青松林耀文
Owner 浙江夸克生物科技有限公司
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