Simple and rapid determination method for alcoholic strength of 50-60DEG wine

A technology for rapid determination and alcohol content, applied in biological testing, color/spectral characteristic measurement, material inspection products, etc., can solve the problems of unsatisfactory rapid detection development, long detection process, poor stability, etc., and achieve low price and good quality Accuracy and reliability, the effect of small wine samples

Active Publication Date: 2018-02-02
黄种山
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The entire detection process is time-consuming, has many steps, and poor stability, which cannot meet the needs of rapid detection development

Method used

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  • Simple and rapid determination method for alcoholic strength of 50-60DEG wine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1 is the making of alcohol content determination standard curve in the simple and quick determination method of alcohol content of 50 to 60 degree wine of the present invention.

[0015] (1) Artificial 50-60% wine sample preparation

[0016] Use corresponding pipettes to take 50.0, 52.0, 54.0, 56.0, 58.0 and 60.0ml of absolute ethanol respectively into a 100ml volumetric flask, continuously add distilled water and repeatedly slightly shake and let stand until the final volume is set to 100ml, and stand at room temperature for 10min. Promptly obtained the artificial 50 to 60 degree liquor samples of different alcohol degrees.

[0017] (2) Manual 50 to 60 degree wine sample pretreatment

[0018] Use a pipette gun to pipette 400ul of the 50-60°C artificial wine sample (30.0°C) to be tested into an empty common microplate, first add 12% mass volume fraction of urea and 15% volume volume fraction of formamide mixed solution 50ul As an initial denaturant, add 50u...

Embodiment 2

[0026] Embodiment 2 is to utilize new method of the present invention to the actual mensuration of certain 50 to 60 degree wine alcoholic strength.

[0027] (1) Pretreatment of wine samples to be tested

[0028] Select commercially available Laojiuhan baijiu (Wenzhou Liulong Liquor Industry Co., Ltd.), and take two bottles (Laojiuhan 1 and Laojiuhan 2) for alcohol determination. Use a pipette gun to pipette 400ul of the wine sample (30.0°C) into an empty common microtiter plate, first add 50ul of a mixed aqueous solution of 12% by mass volume fraction of urea and 15% by volume volume fraction of formamide as an initial denaturant, and then Add 50 ul of the short double-stranded DNA fragment mixture system shown in SEQ ID NO: 1-2 (the concentration of each fragment is 10 μmol / l), the total system is 500 ul, mix well and let stand for 5 minutes.

[0029] At the same time, set the control well as a system of 400ul wine sample to be tested plus 50ul initial denaturant plus 50ul d...

Embodiment 3

[0036] Example 3 is to use the new method of the present invention to measure the alcohol content of commercially available Jiugui Liquor (Jiugui Liquor Co., Ltd.), which is 50 to 60% alcohol, and take two bottles (Jiugui Liquor 1 and Jiugui Liquor 2) in total. Operation with reference to Example 2, the results are shown in Table 3. In addition, the density bottle method is used to measure the alcohol content of this wine.

[0037] Table 3 The alcoholic strength numerical value that method of the present invention and density bottle method measure

[0038] Alcohol

[0039] As can be seen from Table 3, the deviations between the alcohol content of the two bottles of Jiugui liquor measured by the method of the present invention and the measurement results of the density bottle method are 0.26% and 0.31%, respectively, and both meet the accuracy of the alcohol content determination stipulated in the national standard within 1%.

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Abstract

Alcoholic strength is a necessary determination indicator of alcohol products, and accurate and reliable, simple and rapid, economical and practical alcoholic strength determination method is lacked currently. The invention utilizes the principle that melting can occur to DNA fragments in an ethanol-containing system and cause absorbance change under 260nm wavelength and establishes a new alcoholic strength determination method. Directed at 40-50DEG wine, the invention screens out an appropriate short double-stranded DNA fragment hybrid system shown as SEQ ID NO:1-2, establishes a standard curve equation of the absorbance x and wine sample alcoholic strength y as y=8.8697x+48.577 (y is less than or equal to 60 and greater than or equal to 50). The alcoholic strength determination method provided by the invention is suitable for determination of 50-60DEG wine, has the advantages of good accuracy and reliability, no need for distillation and safety, simple and rapid operation, small winesample amount, and low price, and is suitable for 50-60DEG wine alcoholic strength determination practice of wineries.

Description

technical field [0001] The invention relates to an ethanol detection method, in particular to a simple and rapid method for measuring the ethanol content of 50-60% wine, that is, the alcohol content. Background technique [0002] Ethanol is the main component of wine, and its content is one of the important indicators of wine quality. It is called alcohol in the national standard, and it usually refers to the number of milliliters of ethanol contained in every 100 ml of wine at 20 °C. Alcohol level is a must-test indicator for alcoholic products. There are currently many determination methods, common ones include alcohol meter method, density bottle method, gas chromatography and near-infrared spectroscopy. Gas chromatography and near-infrared spectroscopy are two methods based on modern analytical technology, which have the advantages of high detection sensitivity, accurate results, and high-throughput measurement, but they also have disadvantages such as expensive equipmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/98G01N21/33
CPCG01N21/33G01N33/98
Inventor 黄种山黄海霞
Owner 黄种山
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