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Method for detecting hemoglobin concentration in solution by using nitrogen doped carbon quantum dots

A technology for hemoglobin concentration and carbon quantum dots is applied in the field of detecting hemoglobin concentration in a solution by using nitrogen-doped carbon quantum dots, which can solve the problems of limited detection methods, expensive equipment, complicated processing, etc., and achieves a simple and convenient detection process and reduces the detection limit. , the preparation method is simple

Inactive Publication Date: 2018-02-09
GUANGXI TEACHERS EDUCATION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Unfortunately, although the hemoglobin detection technology has been greatly improved, the above detection methods have limitations, such as the use of toxic reagents, expensive equipment, and complicated sample pretreatment.

Method used

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  • Method for detecting hemoglobin concentration in solution by using nitrogen doped carbon quantum dots
  • Method for detecting hemoglobin concentration in solution by using nitrogen doped carbon quantum dots

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Embodiment 1

[0036] A method utilizing nitrogen-doped carbon quantum dots to detect the concentration of hemoglobin in a solution, comprising the following steps:

[0037] Step 1. To prepare nitrogen-doped carbon quantum dots, first add 2.5g of trypsin to 10ml of ultrapure water and mix and stir for 5 minutes to obtain a mixed solution, then transfer the prepared mixed solution to a 25mL reaction kettle for water treatment at 180°C Thermal reaction for 12 hours to obtain the nitrogen-doped carbon quantum dot crude liquid, the obtained nitrogen-doped carbon quantum dot crude liquid was centrifuged at a speed of 6000 rad / min to obtain the supernatant for filtration, filtered with a 0.22 μm filter to obtain the filtrate, and then the filtrate was used A dialysis bag with a molecular weight of 1000Da was dialyzed to obtain a dialysate, and the dialysate was vacuum-dried at 60°C for 24 hours to obtain water-soluble yellow fluorescent nitrogen-doped carbon quantum dots, and finally the obtained n...

Embodiment 2

[0044] A method for detecting the concentration of hemoglobin in a solution using nitrogen-doped carbon quantum dots, the process of which is roughly the same as in Example 1, the difference being that the hemoglobin freeze-dried powder used to prepare the original hemoglobin solution is obtained by the following method: first extract the erythrocytes, add 8 times the volume of 0.8% normal saline to the extracted erythrocytes to obtain a mixed solution, and place it in a warm water bath at 37°C for 2 hours, and pass through the mixed solution at a speed of 45ml / min during the standing period Inject nitrogen gas, then centrifuge for 20 minutes, remove the upper layer solution, retain the deposited red blood cells in the lower layer, complete one centrifugation, then repeat the above centrifugation operation 6 times, collect the deposited red blood cells in the lower layer, add 0.2 times the volume of ultrapure water, and perform ultrasonic crushing, the crushing power 160W, the ...

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Abstract

The invention discloses a method for detecting the hemoglobin concentration in a solution by using nitrogen doped carbon quantum dots. The method comprises the following steps: I, preparing multiple isometric standard solutions which contain the nitrogen doped carbon quantum dots at the same concentration and hemoglobins at different concentrations, and respectively detecting fluorescence intensity values I of the multiple standard solutions; II, respectively preparing contrast solutions which have the same volumes and the same nitrogen doped carbon quantum dot concentrations as the standard solutions in the step I, and detecting fluorescence intensity values I0 of the contrast solutions; III, building a linear relation between (I0-I) / I and the concentrations of the hemoglobins; IV, preparing a to-be-detected solution corresponding to the standard solutions in the step I, detecting a fluorescence intensity value of the to-be-detected solution, substituting the fluorescence intensity value into the linear relation in the step III, and calculating the concentration of the hemoglobins in the solution to be detected. According to the method, a high-sensitivity biosensor for detecting the hemoglobins is constructed by taking the carbon quantum dots as fluorescence probes, so that sensitive and visible detection of the hemoglobins is realized, and a detection limit for the hemoglobins is lowered.

Description

technical field [0001] The invention relates to the field of hemoglobin detection, in particular to a method for detecting hemoglobin concentration in a solution by using nitrogen-doped carbon quantum dots. Background technique [0002] Hemoglobin (HGB) mainly exists in red blood cells and is a tetrameric protein composed of two α-helices and two β-sheet polypeptide chains. As the respiratory protein in red blood cells in the human body, hemoglobin is a reversible oxygen transport and storage protein with a high helical capacity. It has been found through research that changes in the structure of hemoglobin will affect certain functions and pose a certain threat to human health. Changes in hemoglobin may lead to intermolecular interactions, change the shape of red blood cells, cause sickle cell anemia, etc., thereby affecting human health. In addition, the content of hemoglobin in the blood is also related to clinical diseases such as anemia, heart disease, and leukemia. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/28G01N1/38
CPCG01N21/6486G01N1/28G01N1/38
Inventor 黄珊肖琦姚建东杨二利
Owner GUANGXI TEACHERS EDUCATION UNIV
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