Selenium-enriched yeast culture process and optimizing method thereof

Abstract

The invention discloses an optimizing method of a selenium-enriched yeast culture process. A novel sodium selenite adding mode is adopted, production of mycelium is unaffected, the utilization rate ofselenium is greatly increased, inorganic selenium is converted to organic selenium as much as possible, toxicity of the product is reduced, and high industrialized application is achieved; accordingto above results, shaking culture temperature is adjusted to be 23DEG C, initial to pH 6.0 and rotational speed to 120rpm; when a culture medium comprises, by weight, 1.0g / L of magnesium sulfate, 2.0g / L of sodium chloride, 0.5g / L of monopotassium phosphate and 2g / L of sodium selenite, sodium selenite is evenly divided into three equal parts which are added into the culture medium respectively in the first, second and third days of fermentation, about 11.4g dry weight of mycelium and about 331.7mug / g of enriched selenium can be obtained after 7 days of fermentation, and the conversion rate of organic selenium is up to 90.0%. Compared with the prior art, the selenium-enriched yeast culture process and the optimizing method have the advantages that cost is greatly saved and are suitable for modern industrial development.

Description

Technical field [0001] The invention relates to a method for optimizing selenium-enriched yeast culture technology, and belongs to the technical field of selenium-enriched yeast. Background technique [0002] Yeast is a single-celled eukaryotic microorganism with a relatively simple structure. It is rich in amino acids and vitamins, such as methionine, lysine and vitamin C. It is a facultative anaerobic fungus. If there is sufficient oxygen, the bacteria can multiply in large numbers. Under the conditions of lack of oxygen, glucose can be fermented to produce alcohol. It is a very easy to cultivate bacteria and can multiply on ordinary peptone medium. Therefore, in modern industrial production, yeast is widely used, such as winemaking, glycerol production, edible yeast, organic acids, enzyme preparations, etc. Yeast can enrich the trace elements in the culture medium through biological enrichment, so it can be used as a carrier for selenium enrichment. [0003] Selenium (Se) is o...

AI Technical Summary

Problems solved by technology

However, most domestic selenium supplements still use selenite-based inorganic selenium for supplementing selenium. However, due to the disadvantages of low yield and easy poisoning of animals, it cannot be used as the main selenium source for biological selenium supplementation.

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