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A kind of rapid propagation method of rough rib tissue culture

A tissue culture and rapid technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low multiplication coefficient, lack of market value, and a large number of female parents, and achieve stable genetic traits and production. The effect of low cost and rapid proliferation in quantity

Active Publication Date: 2020-12-25
ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current production is still based on conventional terminal bud cutting cuttings as the main propagation method.
The characteristics of the propagation method of terminal bud cutting cuttings determine its great limitations: it has obvious seasonality, requires a large number of female parents, requires a lot of manpower and cultivation facilities, and it is difficult to continuously provide commercial seedlings with stable specifications
The above aspects have resulted in the abnormal situation of hoarding and not selling coarse rib grass, and selling it without successors, resulting in the abnormal situation that there is no market for the price, which seriously restricts the development of the industry.
In recent years, some researchers have studied the tissue culture rapid propagation and regeneration of some Sclerosia species. However, due to the low multiplication coefficient, it is difficult to meet the technical requirements for mass production, and because of the different ornamental traits of Sclerosia species. There are great differences in reproductive traits among them, and it is found that the higher the degree of color leaves and the lower the proportion of green leaves, the more difficult it is to reproduce, and the more difficult it is to propagate and multiply by tissue culture
At present, there is no technical research data on tissue culture and rapid propagation technology of S.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, rapid propagation of tissue culture of Aglanonema spp. 'lady' one:

[0037] In this example, a color-leaved variety 'Lady' of Aglanonema spp. was selected for rapid propagation through tissue culture. The leaf color of this rough rib grass variety is bright red, with good resistance and excellent ornamental properties.

[0038] Select 90 cups of cultivated plants of Aglanonema spp. 'Lady' with excellent properties and strong growth, first place the mother plant in a well-ventilated place, and regularly spray carbendazim 7 days / time, twice After taking materials. Cut the stem from the second bud at the base. After removing the leaf petiole from the base with a scalpel to expose the bud point, it was sterilized by shaking with 75% ethanol solution for 45 seconds and then rinsed with sterile water. Disinfect with bleach solution with 1.5% effective chlorine content + TWEEN-20 (3 drops / liter bleach solution) on the ultra-clean workbench for 18 minutes. Sha...

Embodiment 2

[0044] Embodiment 2, rapid propagation of tissue culture of Aglanonema spp. 'China Red' one:

[0045] In this example, a color-leaved variety 'Red' of Aglanonema spp. was selected for rapid propagation through tissue culture. This variety has excellent plant shape and bright red leaf color and is popular in the market. However, due to the extremely low chlorophyll content in the leaves, it is difficult for cuttings to take root. It is urgent to overcome the mass production problem through tissue culture technology.

[0046] Select 90 cups of cultivars of Aglanonema spp. 'China Red' with excellent properties and robust growth, first place the mother plant in a well-ventilated place, and regularly apply carbendazim for 7 days / time, after spraying twice draw materials. Cut the stem from the second bud at the base. After removing the leaf petiole from the base with a scalpel to expose the bud point, it was sterilized by shaking with 75% ethanol solution for 45 seconds and then r...

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Abstract

The invention provides a method for tissue culture and rapid propagation of aglaonema schott. The method comprises the following steps: after induction and culture of buds, propagation and culture ofcluster buds, subculture of the cluster buds and rooting culture sequentially, obtaining a complete plant; taking out of a bottle, tempering a seeding and transplanting. The method provided by the invention is easy to operate and low in production cost, does not pollute the environment and can realize the large-scale production. The aglaonema schott cultured by the method provided by the inventionis stable in reproductive progeny seedling inheritance, comprehensive in character expression and excellent in ornamental trait. The technical system is low in production cost, environmentally-friendly, free from environmental pollution, and quick in mass production speed and completely realizes the technical requirements for mass production.

Description

technical field [0001] The invention belongs to the technical field of tissue culture rapid propagation, and in particular relates to a tissue culture rapid propagation method of Grass spinosa. Background technique [0002] Aglaonema Schott is a plant belonging to the Araceae family. Coleus coleus has rich ornamental characters, bright and generous leaf color, and has high ornamental value. Moreover, it is resistant to strong light, shade and humidity, and high temperature and heat. It is the first choice for indoor potted plants, hydroponics, and other ornamental plants. The current domestic market research has found that Coleus coleus is a very popular ornamental species with great potential, especially the auspicious Ruyi variety, which is already in short supply. However, the current production is still based on conventional terminal bud cutting cuttings as the main propagation method. The characteristics of the propagation method of terminal bud cutting cutting deter...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 尤毅景维杰钟荣辉刘金梅章金辉陈香罗
Owner ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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