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Industrial pleurotus eryngii cultivation method based on high-activity liquid strains

A technology of liquid strains and cultivation methods, applied in mushroom cultivation, cultivation, plant cultivation, etc., can solve the problems of poor efficiency, many manual operation links, long production cycle, etc., and achieve low price, high cost reduction, and short growth cycle shortened effect

Inactive Publication Date: 2018-02-27
江苏久禾生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The industrialized cultivation of Pleurotus eryngii is a new type of cultivation technology that is rapidly developing in the world. The main differences between the industrialized production of Pleurotus eryngii and the traditional greenhouse seasonal production are: 1. High efficiency: from the construction of the mushroom house to the equipment The configuration is basically designed according to high standards, using mechanized operation, and some of them have adopted automatic control; greatly improving labor efficiency and reducing labor intensity; 2. Annual production: factory production adopts manual control of the growth of Pleurotus eryngii. Temperature, humidity, light, so that it is not affected by external seasonal changes; 3. Large land utilization rate: factory production adopts three-dimensional cultivation, which greatly improves the utilization rate of unit area; the rapid development in my country is only a few short Years, despite continuous improvement in recent years, there are still problems such as long production cycle, many manual operations, and poor efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The present embodiment provides a kind of Pleurotus eryngii industrial cultivation method based on highly active liquid bacterial classification, comprises the following steps:

[0040] Step (1): After the Pleurotus eryngii slant strains are activated on the plate medium, they are successively expanded and cultivated in liquid seed medium, shake flask seed medium and seed tank medium to obtain seed tank seeds, and the seed tank seeds are inserted into the Obtain liquid mother medium from the culture medium;

[0041] The shake flask seed culture medium is the same as the seed tank culture medium, and includes the following components in terms of parts by mass: wheat grains: 50 parts, sawdust: 20 parts, rape stalks: 7 parts, corncobs: 10 parts, bran: 9 parts , brown sugar: 1 part, magnesium sulfate: 0.1 part, lime: 1 part, potassium dihydrogen phosphate: 0.3 part;

[0042] The preparation method of this culture medium is specifically as follows:

[0043] (1) Rinse the u...

Embodiment 2

[0062] The present embodiment provides a kind of Pleurotus eryngii industrial cultivation method based on highly active liquid bacterial classification, comprises the following steps:

[0063] Step (1): After the Pleurotus eryngii slant strains are activated on the plate medium, they are successively expanded and cultivated in liquid seed medium, shake flask seed medium and seed tank medium to obtain seed tank seeds, and the seed tank seeds are inserted into the Obtain liquid mother medium from the culture medium;

[0064] The shake flask seed culture medium is the same as the seed tank culture medium, and includes the following components in terms of parts by mass: wheat grains: 60 parts, sawdust: 30 parts, rape stalks: 9 parts, corncobs: 15 parts, bran: 11 parts , brown sugar: 3 parts, magnesium sulfate: 0.3 parts, lime: 3 parts, potassium dihydrogen phosphate: 0.5 parts;

[0065] The preparation method of this culture medium is specifically as follows:

[0066] (1) Rinse ...

Embodiment 3

[0085] The present embodiment provides a kind of Pleurotus eryngii industrial cultivation method based on highly active liquid bacterial classification, comprises the following steps:

[0086] Step (1): After the Pleurotus eryngii slant strains are activated on the plate medium, they are successively expanded and cultivated in liquid seed medium, shake flask seed medium and seed tank medium to obtain seed tank seeds, and the seed tank seeds are inserted into the Obtain liquid mother medium from the culture medium;

[0087] The shake flask seed culture medium is the same as the seed tank culture medium, and includes the following components in terms of parts by mass: wheat grains: 55 parts, sawdust: 25 parts, rape stalks: 8 parts, corncobs: 13 parts, bran: 10 parts , brown sugar: 2 parts, magnesium sulfate: 0.2 parts, lime: 2 parts, potassium dihydrogen phosphate: 0.4 parts;

[0088] The preparation method of this culture medium is specifically as follows:

[0089] (1) Rinse ...

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Abstract

The invention discloses an industrial pleurotus eryngii cultivation method based on high-activity liquid strains. The method comprises the following steps that pleurotus eryngii slant strains are inoculated on a plate culture medium for activation and culture, hyphae are selected as mother strains and subjected to standing till mycelial pellets germinate, the mycelial pellets are transferred to ashaking table for constant-temperature culture so as to obtain liquid strains, the liquid strains are subjected to step-by-step enlarging culture sequentially through a shake flask seed culture mediumand a seed culture tank, and then seed tank seeds are obtained; fermentation culture liquor is poured into a fermentation tank according to the liquid loading volume, water spraying is conducted forcooling after sterilization, sterile air is introduced, the seed tank seeds are inoculated into the fermentation tank, cooling is slowly conducted after constant-temperature fermentation, the seed tank seeds continue to be inoculated into the fermentation tank, cooling is conducted after fermentation, then constant-temperature fermentation is conducted, and the high-activity liquid pleurotus eryngii strains can be obtained. The method comprises the steps of step-by-step enlarging cuture of the strains, fermentation, loading, inoculation, culture, fruiting, harvesting and packaging, the labor productivity and the qualified rate the product quality are greatly improved, and the economic benefits of pleurotus eryngii cultivation are further improved,.

Description

technical field [0001] The invention belongs to the field of edible fungus cultivation, in particular to the technical field of industrial production of Pleurotus eryngii, in particular to an industrial cultivation method of Pleurotus eryngii based on high-activity liquid strains. Background technique [0002] Pleurotus eryngii is a precious fungus suitable for both medicine and food. Pleurotus eryngii has thick meat, crisp texture and excellent taste. It is one of the 16 rare edible fungi recommended by the World Health Organization and the Food and Agriculture Organization of the United Nations. [0003] The industrialized cultivation of Pleurotus eryngii is a new type of cultivation technology that is rapidly developing in the world. The main differences between the industrialized production of Pleurotus eryngii and the traditional greenhouse seasonal production are: 1. High efficiency: from the construction of the mushroom house to the equipment The configuration is basic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00
Inventor 吴小涛黄晓峰黄应瑞陈泽针
Owner 江苏久禾生物科技发展有限公司