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Method for extracting madecassic acid

A technology of madecassic acid and extraction method, which is applied in the field of biological extraction, and can solve the problems of many consumables, seldom used, and high cost

Active Publication Date: 2018-02-27
无锡戴可思生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of purification, conventional column chromatography is still the mainstream method for purifying triterpenoids. Other methods, such as chromatography, are rarely used due to the large number of consumables and high cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1) Pulverize Centella asiatica, reflux extract with 60v% ethanol solution, the dosage of 60v% ethanol is 5 times of the weight of Centella asiatica, reflux extract once at 40°C for 0.5h each time, filter the extract, and the filtrate Recover the solvent, add water at 40°C to dissolve, filter, and dissolve the filter residue in 50v% ethanol solution to obtain a crude extract;

[0016] 2) Put the crude extract on the XAD-9 macroporous resin column, the column speed of the crude extract is 3BV / h, and elute with an acid solution with a pH value of 2, the elution rate of the acid solution is 1BV / h, and the combined flow liquid and pickling liquid;

[0017] 3) Adjust the pH value of the combined solution to neutral, filter, put the filtrate on the XAD-3 macroporous resin column again, the column speed of the filtrate is 0.5BV / h, elute with ethanol with a volume concentration of 30%, wash with ethanol The detachment rate is 2BV / h, thin-layer chromatography tracking detection,...

Embodiment 2

[0020] 1) Pulverize Centella asiatica, use 70v% ethanol solution to reflux extract, the amount of 70v% ethanol is 10 times the weight of Centella asiatica, reflux extract at 50°C for 3 times, each time for 1h, filter the extract, and recover the filtrate solvent, add 50°C water to dissolve, filter, and dissolve the filter residue in 60v% ethanol solution to obtain a crude extract;

[0021] 2) Put the crude extract on the XAD-9 macroporous resin column, the column speed of the crude extract is 5BV / h, elute with an acid solution with a pH value of 4, the elution rate of the acid solution is 2BV / h, and combine the outflow liquid and pickling liquid;

[0022] 3) Adjust the pH value of the combined solution to neutral, filter, put the filtrate on the XAD-3 macroporous resin column again, the column speed of the filtrate is 1BV / h, elute with ethanol with a volume concentration of 40%, ethanol elutes The speed is 3BV / h, thin-layer chromatography tracking detection, collecting the et...

Embodiment 3

[0025] 1) Pulverize Centella asiatica, reflux extract with 65v% ethanol solution, the dosage of 65v% ethanol is 8 times of the weight of Centella asiatica, reflux extract at 45°C twice, each time for 0.8h, filter the extract, and the filtrate recovering the solvent, adding water at 40-50°C to dissolve, filtering, and dissolving the filter residue in 50-60v% ethanol solution to obtain a crude extract;

[0026] 2) Put the crude extract on the XAD-9 macroporous resin column, the column speed of the crude extract is 4BV / h, elute with an acid solution with a pH value of 3, the elution rate of the acid solution is 1.5BV / h, combine Effluents and pickling solutions;

[0027] 3) Adjust the pH value of the combined solution to neutral, filter, and put the filtrate on the XAD-3 macroporous resin column again, the column speed of the filtrate is 0.8BV / h, elute with ethanol with a volume concentration of 35%, wash with ethanol The detachment rate is 2.5BV / h, thin layer chromatography trac...

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PUM

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Abstract

The invention discloses a method for extracting madecassic acid. The method comprises the following steps: 1) smashing centella asiatica, refluxing and extracting with ethanol solution, filtering extract, recycling a solvent from filtrate, adding water at the temperature of 40-50 DEG C, dissolving, filtering, and dissolving filter residue in 50-60v% ethanol solution, so that crude extract is obtained; 2) purifying the crude extract with an XAD-9 macroporous resin column, eluting with acid liquor, and mixing effluent and pickling solution; and 3) regulating pH value of mixed solution to be neutral, filtering, purifying filtrate with an XAD-3 macroporous resin column, eluting with ethanol with the volume concentration of 30-40%, carrying out thin layer chromatography tracking detection, andcollecting ethanol eluate containing an asiatic acid ingredient; then eluting with ethanol with the volume concentration of 55-65%, carrying out thin layer chromatography tracking detection, collecting ethanol eluate containing madecassic acid, recycling ethanol from madecassic acid eluate, and drying, so that the madecassic acid is obtained. The extracted madecassic acid is high in purity, and yield is high.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to an extraction method from madecassic acid. Background technique [0002] Centella asiatica (Centella asiatica (L.) Urban) is the dried whole herb of Umbelliferae plant Centella asiatica (L.) Urban. It is widely distributed in the south of the Yangtze River Basin. The whole herb is used as medicine and can be harvested all the year round. It has been used in the field for thousands of years. It was first recorded in "Shen Nong's Materia Medica", listed as a middle-grade product, cold in nature, bitter in taste, and pungent. Heatstroke diarrhea, carbuncle sores, bruises, etc. The chemical constituents in Centella asiatica include triterpene saponins, triterpene acids, polyacetylenes and volatile oils. Modern studies have proved that Centella asiatica extract can effectively promote skin damage, local collagen synthesis and metabolism, and has a tradition...

Claims

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Application Information

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IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 陶坤秀
Owner 无锡戴可思生物科技有限公司
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