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A kind of bacillus subtilis and application thereof

A technology of Bacillus subtilis and seeds, applied in the field of Bacillus subtilis and its application, can solve the problems of insufficient utilization and achieve the effects of fast growth, easy availability of ingredients, and good ability to degrade waste protein

Active Publication Date: 2021-03-09
国港通(北京)数字科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, in the current production process of bio-organic fertilizers, one of the main raw materials used is animal manure from poultry breeding and livestock breeding, which can provide a relatively sufficient nitrogen source and combine with biomass such as straw to prepare organic fertilizers. In the process of livestock and poultry breeding, the utilization of animal feathers and hair is not sufficient, and the degradation process requires the action of microorganisms. Microorganisms that efficiently degrade feathers and hair have potential application value

Method used

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  • A kind of bacillus subtilis and application thereof
  • A kind of bacillus subtilis and application thereof
  • A kind of bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for Bacillus subtilis to degrade keratin, specifically as follows:

[0036] (1) Preparation of seed medium: Each liter of seed medium contains 10 g of peptone, 5 g of beef extract, 3 g of sodium chloride, pH 7.0, sterilized at 121° C. for 20 minutes, and then cooled to room temperature for use.

[0037] (2) Inoculate 1 loop of Bacillus subtilis on the slant into the seed medium, and cultivate at 30° C. at a temperature of 150 rpm for 16 hours to the logarithmic growth phase to obtain a seed solution;

[0038] (3) Preparation of fermentation medium, each liter of fermentation medium contains 20 g of poultry feathers, 3 g of potassium dihydrogen phosphate, 1.5 g of dipotassium hydrogen phosphate, 1 g of magnesium sulfate, 1 g of sodium chloride, pH 7.0, and the medium is sterilized at 115 ° C. Cool for 30 minutes and set aside.

[0039] (4) Inoculate the seed solution obtained in step (2) into the fermentation medium of step (3) at a ratio of 3% by volume, and c...

Embodiment 2

[0044] A method for Bacillus subtilis to degrade keratin, specifically as follows:

[0045] (1) Preparation of seed medium: Each liter of seed medium contains 15g of peptone, 6g of beef extract, 3g of sodium chloride, pH 7.2, sterilized at 121°C for 20 minutes and then cooled to room temperature for use.

[0046] (2) Inoculate one loop of Bacillus subtilis on the slant into the seed culture medium, and cultivate at 37° C. and 200 rpm for 20 hours to logarithmic growth phase to obtain seed liquid.

[0047] (3) Prepare fermentation medium: every liter of fermentation medium contains pig hair powder 20g, potassium dihydrogen phosphate 2.5g, dipotassium hydrogen phosphate 1.0g, magnesium sulfate 1g, sodium chloride 2g, pH value 7.2, culture medium after 121 After sterilizing at ℃ for 20 minutes, cool down for later use.

[0048] (4) Inoculate the seed liquid obtained in step (2) into the fermentation medium of step (3) at a ratio of 5% by volume, and cultivate it at 37°C for 60 h...

Embodiment 3

[0053] A method utilizing bacillus subtilis to degrade keratin, the specific steps are as follows:

[0054] (1) Preparation of seed medium: Each liter of seed medium contains 5 g of peptone, 8 g of beef extract, 4 g of sodium chloride, pH 6, sterilized at 121° C. for 20 minutes, and then cooled to room temperature for use.

[0055] (2) Inoculate one ring of Bacillus subtilis on the slant into the seed culture medium, and cultivate at 37° C. and 220 rpm for 10 hours to obtain a seed solution.

[0056] (3) Preparation of fermentation medium: every liter of fermentation medium contains 20g of feathers, 6.0g of potassium dihydrogen phosphate, 5.0g of dipotassium hydrogen phosphate, 3.0g of magnesium sulfate, 1g of sodium chloride, pH 8.5, and the medium is heated at 121°C Cool for 20 minutes after sterilization.

[0057] (4) Inoculate the seed solution obtained in step (2) into the fermentation medium of step (3) at a ratio of 6%, and cultivate it for 20 hours at a temperature of...

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Abstract

The invention discloses a Bacillus subtilis and its application. The Bacillus subtilis is preserved in the China Center for Typical Culture Collection, the preservation date: June 28, 2017, and the preservation number: CCTCC NO: M2017387. The strain can be used to degrade keratin , to obtain protein degradation products and bacteria, containing protease, keratinase and other enzymatic activities, which can be used to develop decomposing agents for organic fertilizer production, and have good application value in the production of decomposing agents and feed microecological preparations.

Description

technical field [0001] The invention relates to a microbial strain and its application, in particular to a bacillus subtilis and its application. Background technique [0002] The recycling of resources plays an important role in reducing environmental pollution and improving the application value of waste. The core issue is to transform waste resources into usable components, such as converting straw into sugar, protein into amino acids, fats, etc. In recent years, based on the concept of circular economy, the comprehensive utilization of waste has been widely advocated. [0003] my country is a country that lacks protein resources, and the recycling of protein waste resources is of great value. Waste poultry feathers, animal hair, waste bones processed by livestock and poultry, and low-value aquatic waste fish bones and processing scraps are not fully utilized efficiently and comprehensively. They are biotransformed into polypeptides, amino acids and used in the preparati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C05F17/20C12R1/125
CPCC05F11/08C12N1/20C05F17/20C12N1/205C12R2001/125Y02W30/40
Inventor 蔡成岗方程冉蔡海莺沙如意葛青毛建卫
Owner 国港通(北京)数字科技有限公司
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