Method for breeding nontoxic puffer fish

Abstract

The invention relates to a method for breeding a nontoxic and safely-eaten puffer fish. The method comprises the following steps: production of a nontoxic puffer fish parent, identification of the nontoxic puffer fish parent, and stable passage of the nontoxic puffer fish parent. In the invention, a technology for screening the nontoxic puffer fish by using mutation at the 401th site of a voltage-gated sodium ion channel Na1.4a is provided for the first time, and a technology for producing the nontoxic puffer fish parent by using ethylnitrosourea mutagenesis is provided for the first time. Themethod is conducive to the large-scale cultivation of nontoxic heritable puffer fish having stable properties, can be applied to artificial breeding, and can promote the consumption of open-culturedpuffer fish in China.

Description

technical field [0001] The present invention relates to the field of aquatic biological technology breeding, in particular to directed breeding of fish, more specifically, using the mutant individual of the gene target site at the key site of tetrodotoxin binding as the parent to produce large-scale stable hereditary low tetrodotoxin Fugu, thus ensuring safe consumption of fugu. Background technique [0002] Puffer fish, or commonly known as puffer fish, is a fish of the porpoise family. Puffer fish meat is delicious but contains highly toxic tetrodotoxin (tetrodotoxin). Therefore, consumption of puffer fish is expressly prohibited in my country. For example, in wild Takifugu obscurus, 1 gram of liver tissue contains more than 1,000 mouse units (MU, 1 MU means the amount of toxin that kills a male mouse weighing 20 g within 30 minutes) tetrodotoxin, which is highly toxic. The accumulation of tetrodotoxin in wild puffer fish mainly comes from the food chain of puffer fish. ...

AI Technical Summary

Problems solved by technology

However, it is precisely because of the low toxicity of artificially farmed puffer fish that it is necessary to strictly control the type of feed and the breeding environment. Once there is a problem in the management of the breeding production, it is difficult to ensure that the puffer fish farmed are all low-toxic, and there is a serious food safety risk.

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