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Method for extracting natural peptide from house fly larvae

A fly maggot and natural technology, which is applied in the field of extracting natural peptides from fly maggots, can solve the problems of low yield, high cost, and complicated extraction method of extracting natural peptides, and achieve high recovery rate, easy operation, and simple process Effect

Inactive Publication Date: 2018-03-16
罗乌支
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the above technical problems, the present invention invents a method for extracting natural peptides from fly maggots, thereby solving the problems of complex operation, high cost and low yield of natural peptides extracted in the prior art

Method used

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  • Method for extracting natural peptide from house fly larvae

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Embodiment 1

[0031] A method for extracting natural peptides from fly maggots, comprising the following steps:

[0032] (1) select culture material to be that the leftovers of farmer's market fish and poultry and duck processing account for 65%, wheat bran accounts for 35% and cultivate 30kg of fly maggots, wherein 22kg artificially cultivates the 4th generation maggots, and 8kg is for introducing wild flies to lay eggs and hatch and cultivate Fly maggots were used as raw materials, and the body surface of the fly maggots was rinsed with a mass concentration of 3‰ potassium permanganate solution for 10 minutes and picked up, then rinsed with clean water to remove the attached potassium permanganate solution, and then dehydrated with a 60-mesh filter cloth three-legged centrifuge until there is no moisture on the body surface;

[0033] (2) The fly maggots obtained after dehydration in step (1) are ground into a slurry by adding deionized water at a solid-to-liquid weight ratio of 1:3, and t...

Embodiment 2

[0040] A method for extracting natural peptides from fly maggots, comprising the following steps:

[0041] (1) select compost to be that the leftovers of farmer's market fish and poultry and duck processing account for 65%, wheat bran accounts for 30kg of cultivated fly maggot 30kg, wherein the 4th generation maggot of artificial cultivation of 30kg is made raw material, and fly maggot body surface is used quality Potassium permanganate solution with a concentration of 3‰ was rinsed for 10 minutes, then rinsed with clean water to remove the attached potassium permanganate solution, and then dehydrated with a 60-mesh filter cloth three-legged centrifuge until there was no moisture on the body surface;

[0042] (2) The fly maggots obtained after dehydration in step (1) are ground into a slurry by adding deionized water at a solid-to-liquid weight ratio of 1:3, and the resulting slurry is passed through a 60-mesh sieve, and then filtered according to 3.5‰ of the weight of the adde...

Embodiment 3

[0049] A method for extracting natural peptides from fly maggots, comprising the following steps:

[0050] (1) select compost to be that the leftovers of farmer's market fish and poultry and duck processing account for 65%, wheat bran accounts for 30kg of cultivated fly maggots, wherein 30kg is made raw material for introducing the fly maggots of wild fly laying eggs hatching cultivation, fly fly Rinse the body surface of the maggot with a potassium permanganate solution with a mass concentration of 3‰ for 10 minutes, then rinse with clean water to remove the attached potassium permanganate solution, and then use a 60-mesh filter cloth three-legged centrifuge to dehydrate until the body surface is free of moisture;

[0051] (2) The fly maggots obtained after dehydration in step (1) are ground into a slurry by adding deionized water at a solid-to-liquid weight ratio of 1:3, and the resulting slurry is passed through a 60-mesh sieve, and then filtered according to 3.5‰ of the wei...

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Abstract

The invention discloses a method for extracting natural peptide from house fly larvae. The method includes the steps: (1) disinfecting house fly larvae body surfaces and dewatering the body surfaces until the body surfaces are free from moisture; (2) adding water, grinding the house fly larvae into pulp, sieving the pulp and then adding sodium hydrogen sulfite and vitamin C to obtain larvae pulp;(3) adjusting a pH (potential of hydrogen) value and performing microwave treatment and heat preservation; (4) filtering the larvae pulp, manufacturing raw materials of chitin by obtained filter residues, adjusting the pH value of obtained filtrate, standing, filtering the filtrate after layering, and performing micro-filtration and two-stage ultra-filtration on the filtrate; (5) performing nano-filtration on the filtrate by a reverse osmosis membrane; (6) discharging, cooling and freezing concentrated solution; (7) freezing and drying the concentrated solution to obtain a product. The methodis simple in operation, the recovery rate of the extracted natural peptide reaches up to 77.33% and is greatly increased, further, a plurality of by-products can be simultaneously prepared by the method, and the method is applicable to large-scale industrial production, simple in process and easy in operation.

Description

technical field [0001] The invention relates to a method for extracting natural peptides, in particular to a method for extracting natural peptides from fly maggots. Background technique [0002] According to relevant data, there are more than 800,000 known insect species in the world, which is equivalent to 3 / 5 of all animal species on the earth and 4 / 5 of the total weight of all terrestrial animals. Insects are highly adaptable, widely distributed, and their living space is expanding day by day. They are the largest underutilized biological resources on earth. The earliest insects utilized by humans were silkworms and bees, with a history of thousands of years. In the past 50 years, the hot spot of insect utilization research is house fly and bigheaded fly maggot in flies. This is because house fly and bigheaded fly maggot have characteristics that other insects do not have. The cycle is short and the output is high; the newly hatched larvae are only 0.07-0.09mg each, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
CPCC07K1/145
Inventor 罗乌支
Owner 罗乌支
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