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Application of calpain inhibitor to preparation of medicine for relieving lung inflammation caused by cigarette smoke

A calpain, lung inflammation technology, applied in the field of biomedicine to achieve the effect of alleviating the increase in inflammation

Inactive Publication Date: 2018-04-20
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is currently no report on whether calpeptin can regulate lung inflammation caused by cigarette smoke

Method used

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  • Application of calpain inhibitor to preparation of medicine for relieving lung inflammation caused by cigarette smoke
  • Application of calpain inhibitor to preparation of medicine for relieving lung inflammation caused by cigarette smoke
  • Application of calpain inhibitor to preparation of medicine for relieving lung inflammation caused by cigarette smoke

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] [Example 1] Calpeptin alleviates the inflammation of bronchial epithelial BEAS-2B cells caused by cigarette smoke extract in vitro

[0035] The concentration of cigarette smoke extract (CSE) at which 50% of the growth of human bronchial epithelial BEAS-2B cells were inhibited (intervened for 24 hours) by CCK-8 kit was calculated to be 18.02%. After the cells were treated with IC50 concentration of CSE for 24 hours, the cells were intervened with different concentrations of calpeptin for 24 hours to extract cell proteins. The expression of Calpain protein in cells was detected by Western Blotting, and the concentration of calpeptin corresponding to the lowest expression level of Calpain protein was 50 nM, which was used for subsequent experiments.

[0036] Conclusion: if figure 1 As shown, calpeptin observed under an inverted microscope alleviated the inflammation of bronchial epithelial BEAS-2B cells induced by cigarette smoke in vitro.

Embodiment 2

[0037] [Example 2] Observing calpeptin under a transmission electron microscope relieves lung inflammation caused by cigarette smoke

[0038]The lung specimens of CS5-3 and CS+C groups were placed in 2.5% glutaraldehyde fixative solution and fixed in a refrigerator at 4°C for 4 h. Post-fixed with 1% osmium tetroxide for 2.5 h, rinsed thoroughly with 0.1 mol / L phosphate buffer, and then underwent routine dehydration. Use gradient ethanol dehydration method, the concentration is 50%, 70%, 90%, 95%, 100%, and then use 1:1 solution, that is, 100% anhydrous acetone: 100% anhydrous ethanol to dehydrate twice. The residence time is 15-20 min. Use epoxy resin Epon812 and 815 to mix embedding agent and acetone at 1:1 and 2:1 concentrations to saturate the sample for 2 hours in each concentration. As time goes by, acetone will gradually volatilize and the purity of embedding agent will increase. Then the cochlea was longitudinally embedded in the pure embedding agent, polymerized in a...

Embodiment 3

[0041] [Example 3] Hematoxylin and eosin staining to observe that calpeptin relieves lung inflammation caused by cigarette smoke

[0042] The lung tissues of mice in CS5-3 and CS+C groups were fixed in 10% formaldehyde solution for 24 hours, dehydrated in 50%, 70%, 90%, 95%, and 100% alcohol in turn, and routinely fixed in paraffin, embedded, Sections were 4 μm, stained with hematoxylin and eosin, sealed with neutral gelatin, and observed under a Japanese Olympus BX51 microscope.

[0043] Result: if image 3 As shown, after 3 months of passive smoking in mice, the infiltration of neutrophils, eosinophils, macrophages, fibrinous exudate, and fibrous tissue hyperplasia were seen in the lungs. Pulmonary telangiectasia, congestion and hemorrhage. The bronchial epithelium proliferates, part of it falls off, and the alveolar cavity collapses. Mice passively smoked for 1 month + received passive smoking + intraperitoneal injection of calpeptin for 2 months, the lung interstitial l...

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Abstract

The invention discloses application of a calpain inhibitor to preparation of medicine for relieving lung inflammation caused by cigarette smoke. After the calpain inhibitor calpeptin is used for performing intraperitoneal injection on a passive smoking mouse and treating BEAS-2B cells treated by cigarette smoke extracts, the increase of lung and in vitro bronchus epithelial cell inflammation caused by cigarette smoke can be obviously relieved. Therefore the calpain inhibitor can be used for preparing medicine for relieving lung inflammation increase caused by cigarette smoke; potential and good application prospects are realized in the field of inflammatory lung disease treatment.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and particularly relates to the application of a calpain inhibitor in the preparation of a medicine for alleviating lung inflammation caused by cigarette smoke. Background technique [0002] Active and passive smoking can cause respiratory damage and induce a variety of diseases, including chronic airway inflammatory diseases characterized by airflow limitation and asthma. Cigarette smoke exposure causes airway inflammatory cell infiltration and the release of various inflammatory mediators, mucus hypersecretion, airway hyperresponsiveness, and aggravates allergic airway inflammation. Airway remodeling is manifested by thickening of airway epithelial cells, goblet cell metaplasia, and thickening of extracellular matrix. Smoking reduces the lung function of mice. The longer the smoking time, the higher the decline in lung function. After quitting smoking, the lung function will improve to var...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/27A61P11/00A61P29/00
CPCA61K31/27
Inventor 陶泽璋陈晨左晶晶陈始明邓玉琴肖伯奎李芬
Owner WUHAN UNIV
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