Application of L-aspartic acid to improvement of sensitivity of bacteria to antibiotics
A technology of aspartic acid and antibiotics, applied in the field of medicine, to achieve good results, good application prospects, and the effect of overcoming bacterial resistance
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Embodiment 1
[0036] Example 1 L-aspartic acid improves the sensitivity of Edwardsiella tarda to kanamycin
[0037] 1. Determination of drug resistance of Edwardsiella tarda EIB202
[0038] Edwardsiella tarda is a Gram-negative short bacillus, which was first reported by Hoshina in 1962 and is related to reddisease of Japanese eels. From the first report to the present, the fungus has caused diseases in more than 20 kinds of fish, such as eel, flounder, tilapia, Chinese soft-shelled turtle, carp, etc., causing huge losses to aquaculture. Edwardsiella tarda is also a common pathogenic bacteria in humans and fish, which directly poses a threat to human health.
[0039] Firstly, the minimum inhibitory concentration of Edwardsiella tarda to various antibiotics was determined. The results showed that the minimum inhibitory concentration of Edwardsiella tarda EIB202 to kanamycin was 12.5 μg / mL, the minimum inhibitory concentration to tetracycline was 125 μg / mL, and the minimum inhibitory concen...
Embodiment 2
[0050] Example 2 L-aspartic acid improves the sensitivity of Edwardsiella tarda to other antibiotics
[0051] In order to know whether the addition of L-aspartic acid can improve the sensitivity of Edwardsiella lentus to other antibiotics other than kanamycin, the bacterial samples were prepared according to the method in step 2 of Example 1. The experiment was divided into 3 groups: 2 control groups (no antibiotics and L-aspartic acid added, only antibiotics added) and 1 experimental group (an antibiotic and L-aspartic acid added). The added antibiotics and their concentration were 1 μg / mL levofloxacin, 400 μg / mL ampicillin, 1 μg / mL baroxacin and 100 μg / mL cefazolin sodium. After 6 hours of action, the number of viable bacteria was counted to calculate the survival rate.
[0052] The result is as image 3 As shown, when L-aspartic acid was added, the sensitivity of Edwardsiella tarda to levofloxacin increased by 19.2 times (survival rate dropped from 50.89% after adding onl...
Embodiment 3
[0054] Example 3 L-Aspartic Acid Improves the Sensitivity of Various Bacteria to Kanamycin
[0055] 1. Determination of drug resistance of Escherichia coli clinical drug-resistant bacteria:
[0056] Escherichia coli is the most important and most abundant bacterium in the intestinal tract of animals, and most of the bacteria isolated clinically are multi-drug resistant. Two strains of Escherichia coli were isolated from pig farms, and their drug resistance was tested.
[0057] The result is as Figure 4A As shown, the minimum inhibitory concentration of Escherichia coli clinical bacteria Y17 to roxithromycin is 625 μg / mL, the minimum inhibitory concentration to tetracycline is 6250 μg / mL, and the minimum inhibitory concentration to gentamicin is 2500 μg / mL, The minimum inhibitory concentration for clindamycin is 25000 μg / mL, the minimum inhibitory concentration for ceftazidime is 0.488 μg / mL, the minimum inhibitory concentration for baloxacin is 62.5 μg / mL, and the minimum i...
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