Layer-by-layer self-assembly film, preparation method and application thereof

A layer-by-layer self-assembly and self-assembly layer technology is applied in tissue regeneration, medical science, prostheses, etc. It can solve the problems of not being able to wear it for a long time, poor oxygen permeability of contact lenses, and corneal epithelial shedding, etc., to achieve Conducive to the smooth repair of wound surface, flexible and convenient processability, and the effect of reducing foreign body rejection

Active Publication Date: 2018-04-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It mainly includes film-type synthetic dressings, foam-type synthetic dressings, spray-type synthetic dressings, and hydrogel-type dressings, but each has the following disadvantages: it is easy to cause the accumulation of exudate under the film, the dressing is opaque and difficult to observe, and it is easy to be dissolved by wound exudate. Poor ventilation, etc.
However, the oxygen permeability of contact lenses is not good, which may cause giant papillary conjunctivitis, corneal epithelial shedding, corneal injury, corneal infection, etc., and should not be worn for a long time
Furthermore, contact lenses are molded products, which are not conducive to loading different types and dosages of drugs for different patients.

Method used

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  • Layer-by-layer self-assembly film, preparation method and application thereof
  • Layer-by-layer self-assembly film, preparation method and application thereof
  • Layer-by-layer self-assembly film, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Prepare 5g / L chitosan and 5g / L heparin solutions with PBS buffer, and filter to sterilize. Prepare an aqueous gelatin solution with a mass fraction of 8%, and turn it into a liquid state at 55°C.

[0036] Spread the gelatin solution evenly on the slide to form a shape consistent with the skin wound, and place the slide on ice until the gelatin solution solidifies.

[0037] Add chitosan solution dropwise on the solid gelatin, react for 1 minute, carefully absorb excess solution from the edge with a negative pressure aspirator, then add heparin solution dropwise, absorb excess solution after reacting for 1 minute, and proceed in this order for 15 minutes cycles. Put the slide in an oven at 55°C, wait until the gelatin turns into a liquid, and take out the self-assembled film, which can be used on the wound as a therapeutic dressing.

[0038] The thickness of the self-assembled film is about 10 microns, which is transparent and breathable. Polymer materials chitosan and ...

Embodiment 2

[0040] Prepare 4g / L polyvinyl alcohol, 4g / L hyaluronic acid aqueous solution, prepare 5% epidermal growth factor aqueous solution, and filter to sterilize. Prepare a gelatin aqueous solution with a mass fraction of 10%, and turn it into a liquid state at 55°C.

[0041] Spread the gelatin solution evenly on the slide to form a shape consistent with the corneal epithelial defect, and place the slide on ice until the gelatin solution solidifies.

[0042]Add polyvinyl alcohol solution dropwise on the solid gelatin, react for 30 seconds, carefully suck off excess solution from the edge with a negative pressure suction device, then add hyaluronic acid solution dropwise, absorb excess solution after reacting for 30 seconds; cycle 5 times. Add polyvinyl alcohol solution dropwise, absorb excess solution after 30 seconds of reaction, then add epidermal growth factor solution dropwise, absorb excess solution after 30 seconds of reaction, and perform 10 cycles in this order of addition. ...

Embodiment 3

[0045] Prepare 5g / L chitosan and 5g / L heparin solutions with PBS buffer, and filter to sterilize.

[0046] After routine disinfection and debridement treatment of diabetic chronic refractory skin wounds, perfuse chitosan solution on the wound surface, react for 1 minute, carefully absorb excess solution from the edge of the wound with a negative pressure aspirator, and then perfuse heparin solution on the wound surface, react for 1 minute After a few minutes, the excess solution was sucked off, and 15 cycles were performed according to this perfusion sequence, and the construction of the self-assembled wound dressing was completed. Depending on the wound repair situation, the wound dressing can be reassembled every 5 or 7 days to promote wound healing.

[0047] The self-assembled film is directly assembled on the wound surface, which fits the wound surface perfectly, and is simple and convenient. The thickness of the film is about 10 microns, which is transparent and ventilat...

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Abstract

The invention discloses a layer-by-layer self-assembly film, a preparation method and application thereof. The layer-by-layer self-assembly film is formed by laying polymer layers with opposite electrical properties and dropwise adding charged therapeutic drugs; the distribution and rearrangement time of polymer in a mode of sucking unreacted solution through a vacuum extractor to produce flowingliquid phase is reduced, a step of washing with clear water is avoided, formed negative and positive ionic polymer layers are smoothly fitted, and the film can be applied to various wound surfaces through design of dies; a 100 to 20000nm ultrathin film can be formed, materials in contact with organisms are obviously reduced, and foreign body rejection reaction is reduced. The film is wide in material selection and drug selection ranges. Types and dosages of drugs can be selected, the effects of antibacterial property, anti-inflammatory property and promotion of wound healing are achieved; thefilm has flexible and convenient processibility, is suitable for the requirements of repairing of various injury wound surfaces, and is stable in product quality and good in reproducibility.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a layer-by-layer self-assembled film and its preparation method and application. Background technique [0002] Layer-by-layer self-assembly technology (layer-by-layer self-assembly, LbL) is a technology that relies on static electricity, hydrogen bonds, chemical bonds and other forces between different substances to assemble different types and functional substances into ultra-thin films in a certain order. . It uses the interaction between substances to alternately adsorb on the surface of the substrate to build a nanometer to micrometer-scale multilayer film. Due to the layer-by-layer assembly, the film structure is easy to control, especially the film thickness can be finely controlled at the nanometer level. The traditional self-assembly technology requires solid materials as the substrate, repeatedly infiltrated in different material solutions, and washed in c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/16A61L27/20A61L27/50A61L27/54A61L27/60
CPCA61L27/16A61L27/20A61L27/50A61L27/54A61L27/60A61L2300/232A61L2300/236A61L2300/404A61L2300/406A61L2300/41A61L2300/602A61L2430/16C08L5/10C08L5/08C08L29/04
Inventor 王本叶婷婷贺川江
Owner ZHEJIANG UNIV
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