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Trichoderma koningiopsis liquid medium

A liquid culture medium, the technology of Trichoderma pseudocorningum, applied in the direction of microorganism-based methods, microorganisms, fungi, etc., can solve the problem of unsatisfactory spore expansion, and achieve the effect of low price, improved quality, and simple ingredients

Pending Publication Date: 2018-04-20
黑龙江省庆东阳光农业生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of liquid culture medium that is suitable for Trichoderma pseudokorningi expanded cultivation, to solve the unsatisfactory defect of existing Trichoderma pseudokoningeni liquid culture medium spore amplification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0010] Specific embodiment one: every liter of Trichoderma pseudokoningeni liquid medium in this embodiment is made up of 10g KNO 3 , 5g KH 2 PO 4 , 2.5g MgSO 4 , 0.02g FeCl 3 , 50g sucrose and the rest of the distilled water composition, pH=6.

Embodiment 1

[0012] Potato glucose liquid medium: 1L is mainly made of 200g potatoes and 20g glucose; peel the potatoes and cut them into small pieces about 0.5mm square, take 200g and put them in a 1500mL beaker and boil for 30min, pay attention to stirring with a glass rod to prevent the bottom from sticking , and then filter with double gauze, take the filtrate and add 20g of glucose, and then make up distilled water to 1000mL.

Embodiment 2

[0014] Ordinary carbon source and nitrogen source liquid medium: 1L is mainly made of 20g sucrose and 3g yeast powder; add 20g sucrose and 3g yeast powder to distilled water, heat to dissolve completely, add distilled water to 1000mL, adjust pH to 7.0-7.2, Sterilize at 121°C for 15 minutes.

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PUM

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Abstract

The invention discloses a trichoderma koningiopsis liquid medium and relates to a fungus liquid medium. The defect of unsatisfactory spore amplification of existing trichoderma koningiopsis liquid medium is solved. The trichoderma koningiopsis liquid medium comprises KNO3, KH2PO4, MgSO4, FeCl3 and sucrose. The trichoderma koningiopsis liquid medium disclosed by the invention can increase the sporeoutput of trichoderma koningiopsis by about 10 times in the same culture conditions. The liquid medium disclosed by the invention has the advantages of simple components, low price and the like, andalso can improve the quality of a trichoderma koningiopsis microbial agent.

Description

technical field [0001] The invention relates to a liquid culture medium for fungi. Background technique [0002] Trichoderma fungi belong to the phylum Deuteromycota, Hypocrea, Order Pyrosporium, and Section Pyroporaceae; the sexual stage is Hypocrea Er. of the phylum Ascomycota. Trichoderma fungi widely exist in natural ecological environments such as plant seeds, root circles, bulbs, leaf circles and soil, and have antagonistic effects on most plant pathogenic bacteria. [0003] In 1932, Weindling found that when Trichoderma lignorum (T.lignorum) was co-cultured with Rhizoctonia solani, Rhizoctonia solani was parasitized by Trichoderma lignorum and died. Weindling considered that the Trichoderma could be used In the biological control of plant soil-borne diseases, the research work on the biological control of Trichoderma began. At present, there are more than 100 kinds of microbial agents containing Trichoderma in the world, which are used by more than 60 countries, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/885
CPCC12N1/14
Inventor 宋清晖宋福强
Owner 黑龙江省庆东阳光农业生物科技股份有限公司
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