Method for biosynthesis of magnolol

A technology of biosynthesis and synthesis method, applied in fermentation and other directions, can solve the problems of many organic solvents, serious chemical pollution, complicated organic chemical production steps, etc., and achieve the effects of warm reaction conditions, high content, and easy industrial transformation.

Active Publication Date: 2018-04-20
嘉兴欣贝莱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Organic chemical production steps are cumbersome, there are too many residual organic solvents, and there is discharge of chemical waste liquid, causing serious chemical pollution
However, the extraction of magnolol from plants is often limited by climate, season and human factors.

Method used

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  • Method for biosynthesis of magnolol
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  • Method for biosynthesis of magnolol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for biosynthesizing magnolol, wherein the synthetic method takes 4-allylphenol as raw material and completes it through the catalysis of laccase, and the method steps are as follows:

[0034] 1) First prepare a 50mM potassium dihydrogen phosphate solution, then use dipotassium hydrogen phosphate to adjust the pH to 6, and filter it for later use;

[0035] 2) Use methanol to configure 50mg / ml 4-allylphenol solution;

[0036] 3) Dissolving laccase in the potassium dihydrogen phosphate solution prepared in step 1), the concentration is 50 mg / ml;

[0037] 4) Configure the reaction system: the volume of the reaction system is 2ml, use the potassium dihydrogen phosphate solution configured in step 1) as a buffer, add the 4-allylphenol solution in step 2) to make the final concentration 4mg / ml , adding the enzyme in step 3) to make the final concentration 1mg / ml;

[0038] 5) The reaction system was placed at 37°C, and the reaction was shaken for 3.5 hours;

[0039]...

Embodiment 2

[0042] A kind of method of biosynthesis magnolol, described synthetic method is with 4-allyl phenol as raw material, finishes through the catalysis of laccase, and described method step is as follows:

[0043] 1) First prepare a 55mM potassium dihydrogen phosphate solution, then use dipotassium hydrogen phosphate to adjust the pH to 6.2, and filter it for later use;

[0044] 2) Use methanol to configure 55mg / ml 4-allylphenol solution;

[0045] 3) Dissolving laccase in the potassium dihydrogen phosphate solution prepared in step 1), the concentration is 55 mg / ml;

[0046] 4) Configure the reaction system: the volume of the reaction system is 3ml, use the potassium dihydrogen phosphate solution configured in step 1) as a buffer, add the 4-allylphenol solution in step 2) to make the final concentration 5mg / ml , adding the enzyme in step 3) to make the final concentration 1.2mg / ml;

[0047] 5) The reaction system was placed at 38°C, and the reaction was shaken for 2 hours;

[0...

Embodiment 3

[0051] A method for biosynthesizing magnolol, wherein the synthetic method takes 4-allylphenol as a raw material and completes it through the catalysis of laccase, and the steps of the method are as follows:

[0052] 1) First prepare a 50mM potassium dihydrogen phosphate solution, then use dipotassium hydrogen phosphate to adjust the pH to 6, and filter it for later use;

[0053] 2) Use methanol to configure 50mg / ml 4-allylphenol solution;

[0054] 3) Dissolving laccase in the potassium dihydrogen phosphate solution prepared in step 1), the concentration is 50 mg / ml;

[0055] 4) Configure the reaction system: the volume of the reaction system is 2ml, use the potassium dihydrogen phosphate solution configured in step 1) as a buffer, add the 4-allylphenol solution in step 2) to make the final concentration 5mg / ml , adding the enzyme in step 3) to make the final concentration 1mg / ml;

[0056] 5) The reaction system was placed at 37°C and shaken for 3 hours;

[0057] 6) centrif...

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Abstract

The invention relates to a method for biosynthesis of magnolol. The method adopts 4-allylphenol as the raw material, and uses laccase as the catalyst for catalysis to complete preparation. The differences between the method and the prior art lie in that: 1) a low-cost compound is taken as the raw material, and the raw material cost of the process is low; 2) on the basis of enzyme reaction, the reaction speed is quick, the selectivity and specificity are good, and the reaction conditions are mild; 3) the process has smaller scale and number of production equipment than other methods, is simplein operation, and is easy for industrial transformation; 4) the method only has separation and purification process in the final step of production, the product purification process is simple, and theproduct has better purity and higher content than general methods; and 5) the synthetic process has no waste liquid emission, is environment-friendly and pollution-free, and can achieve sustainable production. The process adopted by the invention is an excellent industrial production route from the perspectives of economy, environment and occupational health.

Description

technical field [0001] The present invention relates to a kind of biosynthesis method of magnolol, specifically a kind of method of enzymatic catalysis production magnolol. Background technique [0002] Molecular formula of magnolol: C 18 h 18 o 2 ; English name: Magnolol; CAS: 528-43-8; Molecular weight: 266.33; Brown to white fine powder, fragrant, pungent and slightly bitter taste. [0003] In recent years, with the development of modern medicine, it has been found that magnolol has anti-inflammatory, antibacterial, anti-oxidation, anti-tumor and many other pharmacological effects, and has a wide range of applications, such as cosmetics and toiletries. The price of its products is also inseparable from the demand of the international market. [0004] Traditional magnolol production process is divided into Chinese herbal medicine extraction and organic chemical synthesis. Most of the actual market is based on plant extraction. Organic chemical production steps are c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/22
CPCC12P7/22
Inventor 杨月王筱陈贤情江会锋王文
Owner 嘉兴欣贝莱生物科技有限公司
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