Preparing method for protein-binding-toxin-imprinted silica gel absorber and adsorption device

A silica gel adsorption and protein technology, applied in chemical instruments and methods, other chemical processes, blood circulation treatment, etc., can solve the problems of unfavorable industrialization, lack of specificity of protein-binding toxins, complex removal process of template molecules, etc., to achieve improved adsorption The effect of high rate, strong permeability and good adsorption performance

Active Publication Date: 2018-04-24
JAFRON BIOMEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the adsorbents used in the above-mentioned blood purification system lack specificity for the adsorption of protein-bo

Method used

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  • Preparing method for protein-binding-toxin-imprinted silica gel absorber and adsorption device
  • Preparing method for protein-binding-toxin-imprinted silica gel absorber and adsorption device

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Example 1

[0033] The preparation method of the protein-binding toxin imprinted silica gel adsorbent in this embodiment includes the following steps:

[0034] (1) Mix 50mL of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane and 150mL of tetramethoxysilane at 5°C under nitrogen and disperse them evenly;

[0035] (2) Weigh the average pore diameter from 2nm to 3nm, the average particle size from 150μm to 250μm, and the specific surface area to be 600m 2 / g to 800m 2 / g, the pore volume is 0.35cm 3 / g to 0.45cm 3 / g of silica gel 40g, soaked in 100mL of 6mol / L HCl for 12h, washed with deionized water to neutrality, dried in a vacuum drying oven at 40℃ for 12h; Under normal pressure, soak the obtained silica gel into 50mL of 3mol / L Calcium carbonate aqueous solution, vacuum, repeat 4 times, filter and dry; and disperse uniformly in the mixture obtained in step (1);

[0036] (3) According to the mass-volume ratio of indoxyl sulfate, human serum albumin and deionized water as 140.29mg...

Example Embodiment

[0038] Example 2

[0039] The preparation method of the protein-binding toxin imprinted silica gel adsorbent in this embodiment includes the following steps:

[0040] (1) Mix 66.67mL of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane and 133.33mL of tetramethoxysilane at -5°C under nitrogen conditions and disperse uniformly;

[0041] (2) Weigh the average pore diameter from 2nm to 3nm, the average particle size from 150μm to 250μm, and the specific surface area to be 600m 2 / g to 800m 2 / g, the pore volume is 0.35cm 3 / g to 0.45cm 3 / g silica gel 45g, soaked in 100mL 6mol / L HCl for 12h, washed with deionized water to neutrality, dried in a vacuum drying oven at 40℃ for 12h; under normal pressure, soak the obtained silica gel into 50mL of 1.5mol / L calcium carbonate aqueous solution, vacuum, repeat 3 times, filter and dry; and disperse uniformly in the mixture obtained in step (1);

[0042] (3) Prepare 5 mL of protein-bound toxin solution according to the mass-volume ratio of indoxyl su...

Example Embodiment

[0044] Example 3

[0045] The preparation method of the protein-binding toxin imprinted silica gel adsorbent in this embodiment includes the following steps:

[0046] (1) Mix 90 mL of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane and 110 mL of tetramethoxysilane at 0°C and under nitrogen conditions and disperse them uniformly;

[0047] (2) Weigh the average pore diameter from 2nm to 3nm, the average particle size from 150μm to 250μm, and the specific surface area to be 600m 2 / g to 800m 2 / g, the pore volume is 0.35cm 3 / g to 0.85cm 3 / g of silica gel 45g, soaked in 100mL of 6mol / L HCl for 12h, washed with deionized water to neutrality, dried in a vacuum drying oven at 40℃ for 12h; under normal pressure, soak the obtained silica gel in 50mL of 1.8mol / L calcium carbonate aqueous solution, vacuum, repeat 4 times, filter, dry; and disperse uniformly in the mixture obtained in step (1);

[0048] (3) According to the mass-volume ratio of indoxyl sulfate, human serum albumin and deionized...

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Abstract

The invention relates to a preparing method for protein-binding-toxin-imprinted silica gel absorber and an adsorption device. The preparing method includes the following steps that 1, siloxane monomers are mixed and evenly dispersed under the conditions of the temperature lower than the normal temperature and the nitrogen atmosphere; 2, silica gel is activated and loaded with a pore-foaming agent,and is evenly dispersed in the mixture obtained in the step 1; 3, a protein-binding-toxin solution is rapidly and evenly dispersed in the mixture obtained in the step 2, a to-be-reacted solution is obtained, then acid is added, and a reaction is carried out; 4, the mixture obtained in the step 3 is filtered, washed, dried and calcined, and then the protein-binding-toxin-imprinted silica gel absorber is obtained. The preparing method is simple, the protein-binding-toxin-imprinted silica gel absorber is high in toxin adsorption rate, and is particularly suitable for uremia blood perfusion.

Description

technical field [0001] The invention relates to the field of blood purification adsorbents, in particular to a preparation method and an adsorption device of a protein-binding toxin imprinted silica gel adsorbent, which is especially suitable for uremic hemoperfusion. Background technique [0002] It is estimated that more than 1.2 million people worldwide suffer from end-stage renal disease (ESRD), and the number of patients is increasing at a rate of 6% to 7% per year. Uremic syndrome is directly related to patient disability and mortality. Protein-bound toxins account for approximately 24% of known uremic toxins. A large number of studies have shown that protein-bound toxins are involved in the progression of chronic renal failure (CKD), and are the basis of renal interstitial fibrosis and cardiovascular complications of CKD. For example, indoxyl sulfate (IS) and p-cresol sulfate (PCS) are involved in the pathophysiological process of renal interstitial fibrosis, athero...

Claims

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Application Information

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IPC IPC(8): C08G77/26C08J9/26B01J20/26B01J20/30A61M1/36C08L83/08
CPCA61M1/36B01J20/268C08G77/26C08J9/26C08J2383/08
Inventor 董凡许为康陈晓峰
Owner JAFRON BIOMEDICAL
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