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Ultrasonic sound assistant method for simulated digestion of lactoglobulin active peptides and functional food applications

A lactoglobulin and active polypeptide technology, applied in the fields of peptides, peptide sources, food science, etc., can solve the problems of inability to absorb, ignore the digestion and absorption of the gastrointestinal tract, and ignore the influence of the selective absorption of small intestinal epithelial cells, and achieve good resistance. inflammatory effect

Active Publication Date: 2018-04-27
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent "Anti-inflammatory peptide isolated from viscera of wrinkled abalone and its application" (201510594885.7), although the preparation of anti-inflammatory peptide by simulating gastrointestinal digestion method, ignores the influence of small intestinal epithelial cells on its selective absorption
In summary, the current methods for preparing functional polypeptides from β-lactoglobulin ignore the digestion and absorption of the gastrointestinal tract, and cannot truly simulate the digestion of bioactive peptides in the gastrointestinal tract and the absorption of polypeptides by small intestinal endothelial cells

Method used

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  • Ultrasonic sound assistant method for simulated digestion of lactoglobulin active peptides and functional food applications
  • Ultrasonic sound assistant method for simulated digestion of lactoglobulin active peptides and functional food applications
  • Ultrasonic sound assistant method for simulated digestion of lactoglobulin active peptides and functional food applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Add 200 mL of β-lactoglobulin solution with a substrate concentration of 1 g / L into the ultrasonic bag, seal it, and then place the sample ultrasonic bag directly in the ultrasonic reactor for single-frequency (40 kHz) ultrasonic treatment. The ultrasonic treatment time is 30min, the intermittent ratio is 10s / 3s, and the temperature is 25°C.

[0053] Preparation of β-lactoglobulin enzymatic hydrolysis solution: adjust the pH of the above protein solution to 8, add alkaline protease, the ratio of enzyme to substrate is 1:20 (w / w), mix evenly, the enzymatic hydrolysis temperature is 50°C, and the enzymatic hydrolysis time is 2h . After the enzymolysis, adjust the pH of the mixture to 7.0, and inactivate the enzyme in a boiling water bath for 10 minutes, centrifuge to obtain a supernatant, desalt, concentrate, and freeze-dry to powder. The degree of hydrolysis of β-lactoglobulin, protein conversion rate, and the anti-inflammatory activity of its hydrolyzate on EA.hy926 ce...

Embodiment 2

[0057] Add 200 mL of β-lactoglobulin solution with a substrate concentration of 4 g / L into the ultrasonic bag, seal it, and then place the sample ultrasonic bag directly in the ultrasonic reactor for dual-frequency (20 / 28 kHz) ultrasonic treatment. The ultrasonic treatment time is 20min, the intermittent ratio is 10s / 3s, and the temperature is 25°C.

[0058] Preparation of β-lactoglobulin enzymatic hydrolysis solution: adjust the pH of the above protein solution to 7.5, add neutral protease, the ratio of enzyme to substrate is 1:30 (w / w), mix evenly, enzymolysis temperature is 55°C, and enzymolysis time is 4h . After enzymolysis, adjust the pH of the mixture to 7.0, inactivate the enzyme in a boiling water bath for 10 minutes, centrifuge to obtain the supernatant, desalt, concentrate, and freeze-dry to powder. The degree of hydrolysis of β-lactoglobulin, protein conversion rate, and the anti-inflammatory activity of its hydrolyzate on EA.hy926 cells were measured.

[0059] S...

Embodiment 3

[0062] Add 200mL of β-lactoglobulin solution with a substrate concentration of 4g / L into the ultrasonic bag, seal it, then place the sample ultrasonic bag directly in the ultrasonic reactor, and process it with triple frequency (20 / 28 / 40kHz) ultrasonic treatment. The ultrasonic treatment time is 10min, the intermittent ratio is 10s / 3s, and the temperature is 25°C.

[0063] Preparation of β-lactoglobulin enzymatic hydrolysis solution: adjust the pH of the above protein solution to 8, add thermophilic protease, the ratio of enzyme to substrate is 1:50 (w / w), mix evenly, enzymolysis temperature 70°C, enzymolysis time 2h . After enzymolysis, adjust the pH of the mixed solution to 7.0, inactivate the enzyme in a boiling water bath for 10 minutes, centrifuge to obtain the supernatant, desalt, concentrate, freeze-dry into powder, measure the degree of hydrolysis, protein conversion rate, and the effect on EA.hy926 cells anti-inflammatory activity.

[0064]Simulated gastrointestinal...

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Abstract

The invention discloses an ultrasonic sound assistant method for simulated digestion of lactoglobulin active peptides and functional food applications, and belongs to the technical fields of dairy product deep processing and functional food preparation. Ultrasonic pretreatment of beta-lactoglobulin is firstly employed; enzymatic hydrolysis is carried out with protease in order to prepare beta-lactoglobulin anti-inflammatory peptides; activity of the beta-lactoglobulin anti-inflammatory peptides is traced by means of simulation of gastrointestinal tract digestion, and after Caco-2 cells simulate absorption of small intestine epithelial cells, the beta-lactoglobulin anti-inflammatory peptides with high anti-inflammatory activity which are digested by gastrointestinal tracts and absorbed by small intestine inner walls which are simulated by Caco-2 cells are represented. Two kinds of beta-lactoglobulin functional polypeptides with high anti-inflammatory activity which are digested by stomach and intestine and absorbed by small intestine inner walls which are simulated by Caco-2 cells are firstly identified; beta-lactoglobulin hydrolysate has good anti-inflammatory activity for blood vessel endothelial cells is firstly reported.

Description

technical field [0001] The invention provides an ultrasound-assisted simulated digestion method of lactoglobulin active peptide and its application in functional food, which belongs to the technical field of deep processing of dairy products and preparation of functional food, and can be used as a functional product to prepare functional food or medicine. Background technique [0002] Chronic inflammation is involved in the occurrence and development of various diseases in the human body, and is the pathogenic basis of many diseases. Vascular endothelial cells, the single layer of squamous epithelial cells that line the inner surfaces of the heart, blood vessels, and lymphatic vessels, play crucial roles in vascular biology, such as regulation of vascular tone, hemostasis, neutrophil recruitment, hormone transport, and Liquid Filtration etc. Vascular inflammation is one of the key factors leading to endothelial dysfunction and is closely related to a variety of diseases, in...

Claims

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Application Information

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IPC IPC(8): C07K14/47C07K5/107C07K5/083C12P21/06A23L33/18
Inventor 任晓锋张熙梁秋芳侯婷马海乐
Owner JIANGSU UNIV
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