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Soil lead pollution in-situ bioremediation method

A technology for bioremediation and lead pollution, applied in the field of soil remediation, can solve problems such as poor remediation effects of remediation methods, and achieve the effects of easy practical application, broad application prospects, and rich types

Inactive Publication Date: 2018-05-01
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an in-situ bioremediation method for lead pollution in soil, so as to solve the problem of poor remediation effect of existing remediation methods

Method used

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  • Soil lead pollution in-situ bioremediation method

Examples

Experimental program
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Effect test

Embodiment 1

[0033] 1. Preparation of sulfate reducing bacteria liquid:

[0034] The sulfate-reducing bacteria were obtained from the sludge pond of the sewage treatment plant of Southwest University of Science and Technology and the surrounding land. The anaerobic sludge was placed in a 150mL Erlenmeyer flask filled with liquid enrichment medium according to the inoculum amount of 2% until it was almost full, then a small amount of liquid paraffin was added, and the anaerobic culture was carried out at 30°C after sealing. When the culture medium turns ink-colored and smells like rotten eggs from the mouth of the bottle, it indicates that a large number of SRBs have multiplied in the enrichment solution, and then anaerobic separation and culture is carried out by dilution coating-stacked dish interlayer method. After 4-5 days of culture, small black spherical colonies grow on the plate, which are sulfate-reducing bacteria. Pick a single colony and insert it into the liquid medium for anaer...

Embodiment 2

[0043] The cultivation method of the bacterium liquid of the sulfate-reducing bacteria of the present embodiment and Thiobacillus denitrificans is the same as that of Example 1, the difference is that the formula of the culture medium is different, specifically as follows:

[0044] The medium formula of sulfate reducing bacteria is: 0.2g K 2 HPO 4 , 0.5g Na 2 SO 4 , 1.5gCaCl 2 2H 2 O, 1.5gMgSO 4 ·7H 2 O, 1.5g C 3 h 5 o 3 Na, 0.5g yeast extract, 0.5g C1 2 h 7 NO 4 , 0.05g ascorbic acid, 1000mL ultrapure water, pH value 7.2-7.5.

[0045] The medium formula of Thiobacillus denitrificans is: 4.0g Na 2 S 2 o 3 ·5H 2 O, 1.0 g KNO 3 , 0.5gKH 2 PO 4 , 0.2g NaHCO 3 , 0.2g MgCl 2 ·6H 2 Dilute O to 1000mL ultrapure water, pH 6.5-7.2.

[0046] The in-situ bioremediation method of soil lead pollution of the present embodiment:

[0047] The soil with lead ion concentration of 600 mg / kg was configured as a simulation, and the culture of sulfate-reducing bacteria and T...

Embodiment 3

[0050] The cultivation method of the bacterium liquid of the sulfate-reducing bacteria of the present embodiment and Thiobacillus denitrificans is the same as that of Example 1, the difference is that the formula of the culture medium is different, specifically as follows:

[0051] The medium formula of sulfate reducing bacteria is: 1.0g K 2 HPO 4 , 1.5g Na 2 SO 4 , 2.5gCaCl 2 2H 2 O, 2.5gMgSO 4 ·7H 2 O, 2.5g C 3 h 5 o 3 Na, 0.5g yeast extract, 1.5g C1 2 h 7 NO 4 , 0.15g ascorbic acid, 1000mL ultrapure water, pH value 7.3-7.5.

[0052] The medium formula of Thiobacillus denitrificans is: 6.0g Na 2 S 2 o 3 ·5H 2 O, 3.0g KNO 3 , 2.0gKH 2 PO 4 , 1.0 g NaHCO 3 , 0.3g MgCl 2 ·6H 2 Dilute O to 1000mL ultrapure water, pH 7.4-7.5. The in-situ bioremediation method of soil lead pollution of the present embodiment:

[0053] Configure the lead ion concentration as 1000mg / kg lead-containing soil as a simulation, and the cultivation of sulfate-reducing bacteria and ...

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Abstract

The invention discloses a soil lead pollution in-situ bioremediation method and belongs to the technical field of soil remediation. The remediation method comprises the steps that (1) soil and sulfatereducing bacteria liquid are mixed at the solid-to-liquid ratio of 100g:(2-10)ml and are subjected to anaerobic culturing at 25-37 DEG C for 2-10 d; and (2) thiobacillus denitrificans liquid is sprinkled onto the surface of the soil treated in the step (1) at the solid-to-liquid ratio of 100g:(1-10)ml to conduct culturing for 1-15 d. According to the soil lead pollution in-situ bioremediation method, comprehensive treatment on the polluted soil is achieved through regulation and control over the sulfur valence state through different bacteria, so that the efficiency is high, additional devices are not needed, the cost is low, and secondary pollution is avoided.

Description

technical field [0001] The invention relates to the technical field of soil remediation, in particular to an in-situ bioremediation method for soil lead pollution. Background technique [0002] With the rapid development of the global economy and society, environmental pollution has become a worldwide problem. Soil pollution is becoming more and more serious as one of the environmental pollution problems. At the same time, the problem of soil pollution in my country has gradually become prominent. The Soil Pollution Prevention and Control Action Plan, known as the "Soil Ten Measures", was issued on May 28, 2016, which fully reflects the country's increasing emphasis on soil pollution. Heavy metal pollution is one of the important types of soil pollution. Heavy metal pollution can not only cause changes in soil composition, structure and function, but also inhibit crop root growth and photosynthesis, resulting in reduced or even extinction of crop yield. More importantly, ...

Claims

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Application Information

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IPC IPC(8): B09C1/10
Inventor 代群威韩林宝党政赵玉莲王岩
Owner SOUTHWEAT UNIV OF SCI & TECH
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