A kind of soil biological activator and preparation method thereof
A technology of soil biology and activator, applied in the agricultural field, can solve problems such as poor effect, harsh use conditions, and low registration threshold for functional bacterial fertilizers
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Embodiment 1
[0048] Described strain is prepared through the following steps:
[0049] (1) Inoculate Saccharomyces cerevisiae on the slant of wort agar and culture it at 28°C for 100 hours to obtain activated Saccharomyces cerevisiae; then inoculate the activated yeasts into wort liquid medium at 12°BX , at 29°C, under the stirring condition of 100rpm, cultivate for 30h; then inoculate the seed solution obtained from the cultivation into the liquid medium, and cultivate for 30h at 29°C under the stirring condition of 180rpm, to obtain the Saccharomyces cerevisiae seed solution; The live bacteria concentration of the Saccharomyces cerevisiae seed liquid is 250 million / ml; wherein, the liquid culture medium comprises 8% malt extract powder by weight, 3% soybean peptone, 4% glucose, 4% yeast extract ;
[0050] (2) Bacillus natto was inoculated onto the PDA slant, and cultured at 37°C for 85 hours to obtain activated Bacillus natto; then the activated Bacillus natto was inoculated into PDA li...
Embodiment 2
[0055] The seed solution prepared in Example 1 was mixed uniformly according to the volume ratio of 1:1:1:1, and the total viable bacterial concentration of the obtained strain was 550 million / ml.
Embodiment 3
[0057] The seed solution prepared in Example 1 was mixed evenly at a volume ratio of 20:1:1:1, and the total viable bacterial concentration of the obtained strain was 300 million / ml.
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