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Application of OMP (outer membrane protein) VP1243 to prevention and treatment of Vibrio infection

A technology of vibrio infection and outer membrane protein, applied in the field of bioengineering, can solve the problems of few species, the spread of drug-resistant bacteria, and drug residues in aquatic products, so as to reduce losses, have good immunogenicity, and ensure food safety and human health effects

Inactive Publication Date: 2018-05-04
ZHEJIANG SCI-TECH UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] At present, the main way to control infectious diseases caused by pathogenic Vibrio is the use of antibiotics, but because long-term use of antibiotics may cause drug residues in aquatic products and the spread of drug-resistant bacteria, the Ministry of Agriculture has banned the use of antibiotics in aquaculture. There are strict regulations, and there are very few types that can be applied

Method used

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  • Application of OMP (outer membrane protein) VP1243 to prevention and treatment of Vibrio infection
  • Application of OMP (outer membrane protein) VP1243 to prevention and treatment of Vibrio infection
  • Application of OMP (outer membrane protein) VP1243 to prevention and treatment of Vibrio infection

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Embodiment 1

[0027] 1. Design of primers for VP1243 gene

[0028] According to the nucleotide sequence of the VP1243 gene in the NCBI database, using pET-32a(+) as the carrier, design the primer sequence (add the restriction sites of BamHI and XhoI enzymes at both ends of the primer respectively), and the primer information is shown in Table 1 .

[0029] Table 1. PCR Primers

[0030]

[0031] 2. Extraction of Genomic DNA of Vibrio parahaemolyticus

[0032] (1) Pick a single colony of Vibrio parahaemolyticus POR1 and culture it in 5 mL of LBS medium, overnight at 37°C in a shaker;

[0033] (2) Take 2 mL of the bacteria solution from the previous night and place them in two 1.5 mL EP tubes, then centrifuge the sample at 12000 rpm for about 1 min, pour off the supernatant, and collect the precipitate; in each of the previous EP tubes Add 1mL sterile water to suspend the precipitate, then centrifuge twice at 10000rpm, each centrifugation time is 2min;

[0034] (3) Add 200 μL of sterile ...

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Abstract

The invention discloses an application of OMP (outer membrane protein) VP1243 to prevention and treatment of Vibrio infection. The OMP VP1243 has good immunogenicity and antiserum still has a positivereaction after being diluted 5,000 times or more; the OMP VP1243 further has excellent immune protection, and immunized mice infected with pathogenic Vibrio all survive; animal organisms immunized with the OMP not only can produce antibodies, but also can be protected from diseases caused by further infection with corresponding pathogenic bacteria; subunit vaccines, bacterial live vaccines and the like developed with the OMP VP1243 as the target have great significance in preventing and resisting vibriosis and Vibrio enteritis caused by Vibrio infection, loss of aquaculture industry can be reduced effectively, and food safety and human health are guaranteed.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to the application of outer membrane protein VP1243 in preventing and treating Vibrio infection. Background technique [0002] Vibrio is a Gram-negative bacillus or Campylobacter, which is widely distributed in various salinity aquatic environments such as oceans and estuaries, and can also be found in human or fish intestines. Vibrio is the most important pathogenic bacteria of marine animals, and infectious diseases caused by pathogenic Vibrio are prevalent all over the world. The marine aquaculture industry in coastal provinces of my country is well developed, but the main cultured species such as large yellow croaker, perch, grouper, flounder, turbot, European eel, etc. are seriously harmed by vibriosis. At the same time, Vibrio parahaemolyticus is also one of the main pathogenic bacteria that cause gastroenteritis worldwide, and the research on this bacteria...

Claims

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Application Information

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IPC IPC(8): A61K39/106A61P31/04C12N15/70C12N1/21C12R1/63
Inventor 潘建义查振中李守梁李伟燕蔡兴浩
Owner ZHEJIANG SCI-TECH UNIV
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