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Application of das in preparation of medicine for preventing/or treating acute immune liver injury induced by LPS/D-galn

An immune liver injury, acute technology, applied in the direction of drug combination, active ingredients of sulfur/selenium/tellurium, digestive system, etc.

Active Publication Date: 2020-05-19
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, studies have shown that DAS has a certain preventive and protective effect on acute liver injury caused by exogenous compounds, drugs, alcohol, etc., but so far there is no report showing that DAS has a protective effect on acute immune liver injury caused by LPS / D-GalN. protective effect

Method used

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  • Application of das in preparation of medicine for preventing/or treating acute immune liver injury induced by LPS/D-galn
  • Application of das in preparation of medicine for preventing/or treating acute immune liver injury induced by LPS/D-galn
  • Application of das in preparation of medicine for preventing/or treating acute immune liver injury induced by LPS/D-galn

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Experimental program
Comparison scheme
Effect test

Embodiment

[0034] 1 Materials and methods

[0035] 1.1 Materials

[0036] 1.1.1 Main reagents and instruments

[0037] DAS was purchased from Sigma (W204218), LPS was purchased from Sigma (L2630), D-GalN was purchased from Aladdin (G115553), ALT, AST, TBIL kits were purchased from Ningbo Meikang Biotechnology Co., Ltd., corn oil was purchased from the market , HE staining solution was purchased from Biyuntian Company, and absolute ethanol and xylene were purchased from Tianjin Fuyu Fine Chemical Co., Ltd. Beckman AU480 automatic biochemical analyzer, multifunctional frozen horizontal centrifuge (Heraeu Biofuge Stratos), pipette (Eppendorf), EC350-1 tissue embedding machine (Microm), HM 325 paraffin slicer (Thermo), QP section Bleaching temperature controller, Milli-Q ultrapure water system, optical microscope (Olympus).

[0038] 1.2 Method

[0039] 1.2.1 Grouping and handling of experimental animals

[0040]Seventy-five male ICR mice, weighing 18-22 g, were purchased from Jinan Peng...

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Abstract

The invention provides application of DAS (Diallyl Sulfide) in preparation of medicine for preventing / or treating LPS (Lipopolysaccharide) / D-GalN-induced acute immune liver injury. An experiment proves that by adopting the DAS, symptoms of inflammatory cell infiltration, hyperemia and necrosis caused by the LPS / D-GalN-induced acute immune liver injury can be obviously improved; specially, the DASwith low dosage of 50 to 100 micromole per kg cannot cause obvious liver injury and can effectively antagonize increment of aminotransferase and total bilirubin caused by the LPS / D-GalN; a certain medicine concentration effect relation is shown; on the liver tissue cell level, the DAS can obviously relieve the symptoms of inflammatory cell infiltration, hyperemia and necrosis, so that the LPS / D-GalN-induced liver cell injury is obviously reduced.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and in particular relates to the application of diallyl monosulfide (DAS) in the preparation of drugs for preventing and / or treating acute immune liver injury induced by LPS / D-GalN. Background technique [0002] Acute fulminant hepatitis is a serious clinical disease that can be caused by viruses, exogenous chemicals, etc., and has a high mortality rate. Currently, there is no effective prevention or treatment method except liver transplantation. [0003] The pathogenesis of virus-induced liver injury is related to the disorder of the body's immune function. In experimental research, lipopolysaccharide (LPS) and D-Galactosamine (D-GalN) are commonly used to induce acute immunity in experimental animals. Acute liver injury to simulate the clinicopathological symptoms similar to viral hepatitis, and use this model to further develop and study medicinal ingredients that have protective eff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/10A61P1/16
CPCA61K31/10
Inventor 谢克勤厉铭
Owner SHANDONG UNIV