A folic acid targeting carrier loaded with anticancer drugs and its preparation method and application
An anti-cancer drug, folic acid targeting technology, applied in the field of medicine, can solve the problems of energy supply and inability, and achieve the effects of enhancing toxicity, reducing toxicity and reducing organ toxicity
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Embodiment 1
[0038] Example 1 Preparation of folic acid targeting carrier loaded with anticancer drug chloroquine phosphate
[0039] 1. Preparation of Chloroquine Phosphate-Zeolite Imidazole Skeleton Carrier (CQ-ZIF-8)
[0040]The anticancer drug chloroquine phosphate was encapsulated in the zeolite imidazole framework by in situ embedding method, and the chloroquine phosphate-zeolite imidazole framework carrier (CQ-ZIF-8) was synthesized. The specific reaction was as follows:
[0041] (1) Dissolve 50mg of chloroquine phosphate in 5ml of deionized water, mix with zinc nitrate, stir at room temperature for 1min, and use the zinc ions of zinc nitrate and anticancer drugs to form a coordination compound;
[0042] (2) Add 8ml of anhydrous methanol and 4g of 2-methylimidazole, and continue to stir at room temperature for 15min, 2-methylimidazole and the zinc ion of the coordination compound form ZIF-8;
[0043] (3) Centrifuge at a speed of 10000rmp / min for 10min, then wash with anhydrous metha...
Embodiment 2
[0049] The characterization of embodiment 2 carrier FA-PEG / CQ-ZIF-8
[0050] 1. The standard curve of chloroquine phosphate is attached figure 1 shown
[0051] Measure the chloroquine phosphate solution absorbance value of different concentrations at 257nm by ultraviolet spectrophotometry, thus draw figure 1 the standard curve, figure 1 R 2 = 0.9988 reached above 0.99 level, so this standard can be used. However, the range used for the standard curve is 10-40 μg / ml.
[0052] 2. Infrared spectrum detection
[0053] (1) Dry FA-PEG, CQ, ZIF-8, FA-PEG / CQ-ZIF-8, then put them into a mortar, add a certain amount (how much) of KBr, and grind the mixture evenly to The particle size is less than 2 μm, so as not to be affected by scattered light, and then placed in a dryer for drying treatment, and the mixture is pressed into a transparent sheet with a pressure of about 40 MPa on a hydraulic press, and measured on the machine;
[0054] (2) Infrared spectrum (FTIR) as attached F...
Embodiment 3
[0057] The CQ loading rate of ZIF-8 load in the carrier FA-PEG / CQ-ZIF-8 of embodiment 3
[0058] 1. Determination of the CQ loading rate of ZIF-8 loading
[0059] Ultraviolet spectrophotometer detection: consult the literature and know that the characteristic absorption peak of chloroquine phosphate is 254nm
[0060] (1) The specific steps for drawing the standard curve of chloroquine phosphate are as follows:
[0061] ① Preparation of curcumin stock solution
[0062] Accurately weigh 1mg of chloroquine phosphate and place it in a beaker, add an appropriate amount of deionized water to dissolve it, transfer it to a 10mL volumetric flask, and then dilute to the mark with deionized water, and use it as a chloroquine phosphate stock solution for later use;
[0063] ② Measure 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 mL from the stock solution and place them in seven 10 mL volumetric flasks, dilute to the mark with deionized water, and shake well. Using deionized water as the blank...
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