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A kind of preparation method of aqueous phase chemiluminescent light source

A technology of chemiluminescence and hydrogen peroxide, which is applied in the field of chemiluminescence, can solve the problem that horseradish peroxidase is not suitable for chemiluminescence light sources, and achieve the effects of reducing poisoning incidents, economical preparation, and avoiding the use of organic solvents

Active Publication Date: 2020-09-22
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, considering storage and cost issues, horseradish peroxidase is not suitable as a catalyst for aqueous chemiluminescent light sources

Method used

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  • A kind of preparation method of aqueous phase chemiluminescent light source
  • A kind of preparation method of aqueous phase chemiluminescent light source
  • A kind of preparation method of aqueous phase chemiluminescent light source

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Synthetic Tris-Co (II) complex

[0022] Add 0.2906g of Tris and 10mL of ultrapure water to a 50mL beaker and sonicate for 5 minutes to obtain a 240mM Tris aqueous solution; add 11.6mg of Co(NO 3 ) 2 ·6H 2 O and add ultrapure water to 50mL, and sonicate for 5 minutes to prepare a 0.8mM cobalt nitrate solution. Take 5mL Tris solution and 5mL Co(NO 3 ) 2 ·6H 2 O in a 50mL beaker, stirred at room temperature for 30 minutes to obtain a beige solution. Take 5 mL of the solution, centrifuge at 10,000 rpm for 5 minutes, remove the supernatant, and retain the beige solid precipitate. Add an appropriate amount of deionized water to wash, centrifuge again to remove the supernatant and retain the precipitate, and repeat twice. The obtained beige solid precipitate was placed in a vacuum oven at 25°C and dried for 4 hours to obtain a dry beige solid powder, which was pulverized with a mortar and then stored. Get 0.1g taupe powder and carry out X-ray energy spect...

Embodiment 2

[0023] Embodiment 2: Tris-Co (II) complex reacts with hydrogen peroxide in aqueous solution

[0024] Take 900 μL Tris-Co(II) complex solution, put it in a 1.5 mL centrifuge tube, add 10 μL DMPO (hydroxyl radical scavenger), and then add 100 μL (50 mM) H 2 o 2 water solution to start the experiment. The reaction solution in the centrifuge tube was taken twice every 5min using a capillary tube and used for electron paramagnetic resonance (ESR) spectroscopy ( image 3 ) to test the content of hydroxyl radicals. After 50 minutes of testing and analyzing the data, we have proved the continuous generation of hydroxyl radicals in the system.

Embodiment 3

[0025] Embodiment 3: Contrast Luminol-Tris-Co(II)-H 2 o 2 Test method of chemiluminescence kinetic curve with traditional peroxalate system:

[0026] Put 100 μL of 10 mM luminol solution and 200 μL of Tris-Co(II) complex solution in a BPCL reaction bottle, and mix well. Adjust the BPCL operating voltage to -720V to ensure the stable operation of the BPCL ultra-weak chemiluminescence analyzer. Close the top cover of the BPCL detector and inject 100 μL of 20 mM H into the system 2 o 2 Aqueous solution, data collected and recorded by BPCL.

[0027] Comparative Test:

[0028] Put 100 μL of 10 mM CPPO solution, 100 μL of 40 μM BPEA solution, and 1M sodium salicylate solution in the BPCL reaction bottle, shake and mix, and adjust the operating voltage of BPCL to -720V. Close the top cover of the BPCL detector and inject 100 μL of 200 mM H into the system 2 o 2 Aqueous solution, data collected and recorded by BPCL.

[0029] The chemiluminescence kinetic curves of the two sys...

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Abstract

The invention relates to a method for preparing an aqueous chemical luminescence light source and belongs to the technical field of chemical luminescence. According to the method, trihydroxymethyl aminomethane (a complex of Tris and bivalent cobalt ions) serves as a catalyst, by using the reaction characteristic that the Tris-Co(II) complex reacts with hydrogen peroxide to produce hydroxyl free radicals (.OH) and the property that luminol reacts with .OH to emit light, long-time and high-strength chemical luminescence is generated, and then, the production of a novel chemical luminescence light source is completed. The chemical luminescence light source disclosed by the invention has the advantages of rapidness, readily available raw materials, simple equipment, simplicity and easiness inoperation and low cost. The chemical luminescence light source has a broad application prospect in some environments where electric energy or energy of other forms cannot be supplied.

Description

technical field [0001] The invention belongs to the technical field of chemiluminescence, and relates to a preparation method of a novel water phase chemiluminescence light source. Background technique [0002] Chemiluminescence is the phenomenon of light radiation accompanying chemical substances in the reaction process. Chemiluminescence does not require the stimulation of external energy, and luminescence can be obtained only by chemical reactions in the system. Therefore, the use of chemiluminescence technology is particularly important in some environments where electric energy or other forms of energy cannot be supplied. At present, chemiluminescent light sources have been widely used in biological imaging, photodynamic therapy, field exploration and some commercial activities. [0003] Commercial chemiluminescent light sources mainly use peroxyoxalate compound-hydrogen peroxide system, and various colors can be obtained by adding different fluorescent dyes to the sys...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C213/08C07C215/10C09K11/06
CPCC07C213/08C09K11/06C09K2211/1007C09K2211/187C07C215/10
Inventor 吕超李哲王连英
Owner BEIJING UNIV OF CHEM TECH
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