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Tissue culture rapid propagation method of pelargonium graveolens

A geranium geranium and tissue culture technology, applied in the field of plant tissue culture, can solve the problems of high production cost of hybrid seeds, achieve the effects of maintaining strain advantages, low tissue browning rate, and easy-to-obtain materials

Active Publication Date: 2018-05-11
SHANDONG CROP GERMPLASM CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production cost of hybrid seeds is high. If tissue culture is used for propagation, the propagation speed can be greatly improved, the cost can be reduced, and the detoxification and rejuvenation can provide an effective and rapid propagation method for Geranium geranium.

Method used

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  • Tissue culture rapid propagation method of pelargonium graveolens
  • Tissue culture rapid propagation method of pelargonium graveolens

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Embodiment 1

[0031] A tissue culture rapid propagation method of Pelargonium geranium, the steps are as follows:

[0032] (1) Selection and disinfection of explants

[0033] Select healthy young stems with good growth and no pests and diseases, remove the leaves, rinse with clean water for one hour, then wash with cleaning solution for 15 minutes, then soak with carbendazim for 10 minutes, then disinfect with 75% ethanol for 30 seconds, and rinse with sterile water for 4 seconds ~5 times, wash for 1 min each time, to obtain sterile explants;

[0034] The cleaning solution contains 1 g of fatty alcohol polyoxyethylene ether sodium sulfate, 1.5 g of ethyl phenyl polyethylene glycol, 0.02 g of zinc chloride, and 2 g of pullulan per L;

[0035] (2) Induction of callus

[0036] Cut the sterile explants in step (1) into small pieces of 0.5-1.0 cm, inoculate them on the callus induction medium and culture them in the dark at 26±1°C for 4 weeks to obtain callus tissue;

[0037] The callus induc...

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Abstract

The invention belongs to the technical field of plant tissue culture and particularly relates to a tissue culture rapid propagation method of pelargonium graveolens. The method comprises selecting pelargonium graveolens tender stems in the good growth condition as explants, disinfecting the explants, cutting the explants into small sections, carrying out callus induction, transferring the inducedcallus into a differentiation medium, inducing the generation of buds, inoculating a novel differentiation medium with the buds, inducing to produce bud leaves, inoculating a rooting medium with the bud with leaves, carrying out rooting culture, and carrying out seeding hardening and transplantation. The tissue culture rapid propagation method greatly improves the propagation speed, reduces the cost, can better maintain the pelargonium graveolens advantages and can provide an effective way for the rapid propagation of pelargonium graveolens. Through compounding and selection of various kinds of culture mediums, the browning and material pollution in the tissue culture are significantly reduced, the success rate of callus induction is high and fast propagation is realized. Through the cooperation of various conditions, the rooting rate and survival rate are improved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a tissue culture rapid propagation method of geranium geranium. Background technique [0002] Geranium ( Pelargonium crispum 'Fragrans' ), also known as fragrant leaf foreign hydrangea, vanilla, and Momoxiang, is a perennial subshrub of the family Geranium and geranium, and is often used as an indoor potted ornamental plant. Fragrant oil can be extracted from the stems and leaves of Geranium geranium, which is an important raw material for soap, perfume, cosmetics and food. The whole plant of Geranium geranium can be used as medicine, and the essential oil of vanilla has anti-tumor effect. In addition, it also has anti-chlorine properties and can be used as an indicator plant for an environment polluted by chlorine. Pelargonium geranium is mainly propagated by cuttings and sexual propagation. The management of cuttings is difficult and limited by seasons. Cutting s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 郭秀秀丁汉凤李娜娜蒲艳艳宫永超
Owner SHANDONG CROP GERMPLASM CENT
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