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Culture medium for improving freezing tolerance of Lactobacillus bulgaricus and application of culture medium

The technology of a culture medium and Lactobacillus is applied in the field of medium preparation for improving the freeze resistance of Lactobacillus bulgaricus, and can solve the problems of poor storage stability at room temperature, low viable count of bacteria powder, and low freeze-drying survival rate, and achieves the survival rate. It is not easy to reduce, the number of viable bacteria is high, and the effect of freeze-drying survival rate improvement

Active Publication Date: 2018-05-11
内蒙古双奇药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of culture medium and application method thereof that improves the freeze resistance of Lactobacillus bulgaricus, this medium can significantly improve the freeze resistance of Lactobacillus bulgaricus, solves the problem that Lactobacillus bulgaricus freezes and survives in the freeze-drying process The rate is not high, the number of viable bacteria in the bacteria powder is low, and the problem of poor storage stability at room temperature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) 3g of anhydrous calcium chloride, 0.5g of magnesium sulfate, 0.1g of manganese sulfate and 0.6g of L-cysteine ​​hydrochloride that were accurately weighed were dissolved in 50mL of distilled water respectively; (2) accurately Weighed 30g mannose, 10g trehalose, 6g casein peptone, 10g beef extract powder, 3g anhydrous sodium acetate, 5g potassium acetate and 1mL Tween-80 were dissolved in 800mL of distilled water and heated to dissolve; (3) Add the four solutions obtained in (1) to (2), stir evenly, adjust the pH to 7.2-7.4, and then sterilize at 121°C for 15-20min. A medium for improving the freeze resistance of Lactobacillus bulgaricus can be obtained.

[0024] The activated Lactobacillus bulgaricus strains were respectively inserted into the freeze-resistant medium of Lactobacillus bulgaricus and the production medium (control group) of Lactobacillus bulgaricus of Inner Mongolia Shuangqi Pharmaceutical Co., Ltd. according to the inoculation amount of 4%. Anaerobi...

Embodiment 2

[0026] (1) 2.4g anhydrous calcium chloride, 0.4g magnesium sulfate, 0.1g manganese sulfate and 0.6g L-cysteine ​​hydrochloride that will be accurately weighed are dissolved in the distilled water of 50mL respectively; (2) will Accurately weighed 20g mannose, 8g trehalose, 4g casein peptone, 8g beef extract powder, 2.4g anhydrous sodium acetate, 4g potassium acetate and 0.8mL Tween-80 were dissolved in 800mL of distilled water and heated to dissolve; ( 3) Add the four solutions obtained in (1) into (2), stir evenly, adjust the pH to 7.2-7.4, and then sterilize at 121°C for 15-20min. A medium for improving the freeze resistance of Lactobacillus bulgaricus can be obtained.

[0027] The activated Lactobacillus bulgaricus strains were respectively inserted into the freeze-resistant medium of Lactobacillus bulgaricus and the production medium (control group) of Lactobacillus bulgaricus of Inner Mongolia Shuangqi Pharmaceutical Co., Ltd. according to the inoculation amount of 4%. An...

Embodiment 3

[0029] (1) 4.0g of anhydrous calcium chloride, 0.8g of magnesium sulfate, 0.5g of manganese sulfate and 1g of L-cysteine ​​hydrochloride that were accurately weighed were dissolved in 50mL of distilled water respectively; (2) accurately Weighed 30g mannose, 10g trehalose, 6g casein peptone, 10g beef extract powder, 6g anhydrous sodium acetate, 6g potassium acetate and 1.2mL Tween-80 were dissolved in 800mL of distilled water and heated to dissolve; (3) Add the four solutions obtained in (1) to (2), stir evenly, adjust the pH to 7.2-7.4, and then sterilize at 121°C for 15-20min. A medium for improving the freeze resistance of Lactobacillus bulgaricus can be obtained.

[0030] The activated Lactobacillus bulgaricus strains were respectively inserted into the freeze-resistant medium of Lactobacillus bulgaricus and the production medium (control group) of Lactobacillus bulgaricus of Inner Mongolia Shuangqi Pharmaceutical Co., Ltd. according to the inoculation amount of 4%. Anaero...

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Abstract

The invention provides a culture medium for improving the freezing tolerance of Lactobacillus bulgaricus and application of the culture medium. The culture medium contains the following components byweight: 12g-30g of mannose, 5g-10g of mycose, 3g-6g of casein peptone, 3g-6g of anhydrous sodium acetate, 5g-10g of beef extract powder, 3g-6g of potassium acetate, 1.5g-4.0g of anhydrous calcium chloride, 0.1g-0.8g of magnesium sulfate, 0.1g-0.5g of manganese sulfate, 0.1g-1g of L-cysteine hydrochloride, 0.6mL-1.2mL of Tween-80 and 1000mL of distilled water. By utilizing the freezing-resistant culture medium and particularly a freeze-drying protection method provided by the invention, relatively high freeze-drying survival rate of Lactobacillus bulgaricus can be realized, the preservation period is relatively long, and the survival rate is unlikely to be decreased.

Description

technical field [0001] The invention belongs to the technical field of production of Lactobacillus bulgaricus products, and relates to the preparation and application method of a culture medium for improving the freeze resistance of Lactobacillus bulgaricus. Background technique [0002] Lactobacillus bulgaricus taxonomically belongs to Lactobacillus genus, Lactobacillus delbrueckii subspecies Lactobacillus delbrueckii species. It is widely used in the fields of medicine and fermented food because of its strong ability to produce acid, and can produce natural antibacterial substances and flavor substances. [0003] Vacuum freeze-drying technology is one of the most common and effective methods for preparing probiotic powder. Due to a series of effects such as mechanical damage, solute damage, and cell membrane damage on lactic acid bacteria during the freeze-drying process, the The survival rate in the country is greatly threatened. The freeze-drying survival rate is relat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12R1/225
CPCC12N1/04
Inventor 黄少磊刘彦民夏少明李林泉刘云辉李晓军李康宁郭跃东马跃英邢会霞王潇焦志军王春颖冯谦龚虹程超马晓红王海霞杨飞雁田鑫张春霞韩至赵晓燕
Owner 内蒙古双奇药业股份有限公司
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