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Escherichia coli culture medium and preparation method thereof

A technology of Escherichia coli and culture medium, which is applied in the field of Escherichia coli culture medium and its preparation, and can solve problems such as high cost and unsuitable for mass preparation

Active Publication Date: 2020-10-27
GUANGZHOU GENSOURCE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this medium requires a large amount of peptone and yeast powder, which is expensive and not suitable for large-scale preparation. Currently, it only stays in the stage of laboratory use

Method used

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  • Escherichia coli culture medium and preparation method thereof
  • Escherichia coli culture medium and preparation method thereof
  • Escherichia coli culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The present embodiment provides a culture medium for Escherichia coli consisting of the following raw materials:

[0043] Egg liquid 150g; papaya peel 80g; soybean powder 12g; glucose 0.25g; deionized water 1000ml.

[0044] The preparation method of above-mentioned escherichia coli culture medium, comprises the following steps:

[0045] (1) Take each raw material according to the raw material ratio of the above-mentioned Escherichia coli culture medium, grind and mix;

[0046] (2) Keeping the pulverized raw materials at 55° C. for 8 hours to obtain a mixture;

[0047] (3) The mixture is boiled, precipitated, filtered with 300 mesh gauze to obtain the turbid liquid of the E. coli culture medium, and then filtered with a 0.22 μm fiber membrane to obtain the supernatant liquid of the E. coli culture medium;

[0048] (4) constant volume, add glucose to the Escherichia coli culture medium supernatant, and settle to 1000ml with deionized water;

[0049] (5) Use NaOH / HCl so...

Embodiment 2

[0071] The present embodiment provides a culture medium for Escherichia coli consisting of the following raw materials:

[0072] Egg liquid 120g; papaya peel 60g; soybean powder 10g; glucose 0.15g; deionized water 1000ml.

[0073] The preparation method of above-mentioned escherichia coli culture medium, comprises the following steps:

[0074] (1) Take each raw material according to the raw material ratio of the above-mentioned Escherichia coli culture medium, grind and mix;

[0075] (2) Keeping the pulverized raw materials at 55° C. for 8 hours to obtain a mixture;

[0076] (3) The mixture is boiled, precipitated, filtered with 200 mesh gauze to obtain the turbid liquid of the E. coli culture medium, and then filtered with a 0.45 μm fiber membrane to obtain the supernatant liquid of the E. coli culture medium;

[0077] (4) constant volume, add glucose to the Escherichia coli culture medium supernatant, and settle to 1000ml with deionized water;

[0078] (5) Use NaOH / HCl so...

Embodiment 3

[0100] The present embodiment provides a culture medium for Escherichia coli consisting of the following raw materials:

[0101] Egg liquid 100g; papaya peel 55g; soybean powder 8g; glucose 0.3g; deionized water 1000ml.

[0102] The preparation method of the above-mentioned Escherichia coli culture medium is the same as that of Example 1.

[0103] The method for cultivating escherichia coli in the above-mentioned escherichia coli culture medium comprises the following steps:

[0104] Preparation of seed liquid: under sterile conditions, take a preserved Escherichia coli slant, use an inoculation loop to inoculate it into a test tube containing 5ml of the Escherichia coli medium, and cultivate it in a constant temperature shaker at 37°C and 200rpm after inoculation After 12 hours, the seed culture solution was obtained, and the seed culture solution was stored at 4°C for future use;

[0105] Expanded cultivation of seed liquid: under aseptic conditions, the described seed cul...

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Abstract

The invention relates to an Escherichia coli medium. The medium comprises 10-15% of egg liquid, 0.7-8% of a protease composition A, 0.8-1.5% of a protein composition B, 0.01-0.03% of a sugar composition C, and the balance of deionized water. The raw materials of the medium are common components in daily life, and have wide sources, so the medium has the advantages of low cost, short culture period, and easiness in obtaining of an ideal high-concentration Escherichia coli liquid.

Description

technical field [0001] The invention relates to the field of microbial culture medium, in particular to an Escherichia coli culture medium and a preparation method thereof. Background technique [0002] Escherichia coli is a type of bacteria that is very closely related to our daily life. The scientific name is "Escherichia coli". Due to its simple structure, the genome has been sequenced and is often used as a biological experimental material and widely used in scientific research. [0003] The culture medium is an artificially prepared nutrient for the growth and maintenance of microorganisms, plant tissues and animal tissues. Due to the different types and research purposes of microorganisms, plant tissues and animal tissues, there are differences in the preparation of the composition of the culture medium. [0004] In existing schemes, LB liquid medium is often used for cultivating bacteria. LB medium is the name of a medium. In biochemical molecular experiments, this m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/19
CPCC12N1/20
Inventor 涂桂洪邓惠平潘忠林谭志康
Owner GUANGZHOU GENSOURCE BIO TECH CO LTD
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