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DNA-directed gold nanocrystal as well as preparation method and application thereof

A gold nanometer and gold nanorod technology, which is applied in the field of DNA-guided gold nanocrystals and their preparation, can solve rare problems, and achieve the effects of reducing preparation cost, promoting technology development, and simplifying preparation process.

Inactive Publication Date: 2018-05-18
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] There have been a lot of literature reports on the preparation methods of DNA with primary structure, that is, single-stranded DNA-guided formation of nanoparticles with special morphology. There are few related reports

Method used

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  • DNA-directed gold nanocrystal as well as preparation method and application thereof
  • DNA-directed gold nanocrystal as well as preparation method and application thereof
  • DNA-directed gold nanocrystal as well as preparation method and application thereof

Examples

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Embodiment 1

[0038] In this embodiment, the preparation of DNA-guided gold nanocrystals comprises the following steps:

[0039] (1) Preparation of guide solution: according to the molar ratio of Au:DNA being 1:600, take a corresponding amount of gold nanorod aqueous solution and DNA solution and mix evenly, the aspect ratio of the gold nanorods in the gold nanorod aqueous solution is 3.6, The DNA solution is a solution of hairpin DNA molecules. Then add sodium chloride to the mixed solution until its molar concentration is 0.5mol / L, then age for 48h to obtain a gold nanorod / DNA complex solution, which is then centrifuged, and then the separated gold nanorod / DNA complex solution The DNA complex pellet was resuspended in 1.5 mL of pure water, and the resulting solution was used as a guide solution for later use.

[0040] (2) Preparation of growth solution: Take 100-1000 μL of 2% tetrachloroauric acid aqueous solution and add it to 10-80 mL of water. After mixing evenly, add sodium hydroxide...

Embodiment 2

[0046] The steps and operating conditions for preparing DNA-guided gold nanocrystals in this example are basically the same as in Example 1, except that the DNA solution used in step (1) is a solution of single-stranded DNA molecules with primary structure.

Embodiment 3

[0048] The steps and operating conditions for preparing DNA-guided gold nanocrystals in this example are basically the same as in Example 1, except that the DNA solution used in step (1) is a solution of double-stranded DNA molecules with secondary structure.

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Abstract

The invention relates to a preparation method of a DNA-directed gold nanocrystal. The preparation method comprises the following steps of firstly, modifying the surface of a gold nanorod with DNA molecules to prepare a gold nanorod / DNA compound, and then enabling tetrachloroauric acid and a reducing agent to generate in-situ reduction reaction in a solution system under the direction action of thegold nanorod / DNA compound to produce the gold nanocrystal. The invention also relates to the DNA-directed gold nanocrystal prepared by the preparation method. The position of a surface plasma resonance maximum absorption peak of the DNA-directed gold nanocrystal ranges between a 780nm visible light region and a 1100nm second infrared region. The invention also relates to application of the DNA-directed gold nanocrystal in malignant tumor photothermal therapy. Compared with the prior art, the preparation method of the DNA-directed gold nanocrystal has the advantages of easiness in realization,low preparation cost and capability of regulating the morphology and the performance of the gold nanocrystal.

Description

technical field [0001] The invention relates to the technical field of nanomaterial preparation, in particular to a DNA-guided gold nanocrystal and its preparation method and application. Background technique [0002] In recent years, it has become an important direction in the field of nanomaterial preparation technology to use deoxyribonucleic acid (DNA) chains to regulate the assembly of gold nanoparticles to generate nanomaterials with special morphology and excellent properties. Gold nanoparticles are widely used in the fields of spectroscopy, biomedicine, environment and catalysis because of their excellent optical, electrical, catalytic and biocompatibility properties. The performance of gold nanoparticles is related to the shape, surface roughness and size of the particles, so the research on the preparation of gold nanoparticles with controllable shape and size has always been a major focus of scientific research workers. [0003] The study found that using the sca...

Claims

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Application Information

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IPC IPC(8): C30B29/02C30B7/14B22F9/24B82Y40/00
CPCB22F9/24B82Y40/00C30B7/14C30B29/02
Inventor 张卓旻高嘉敏李攻科
Owner SUN YAT SEN UNIV
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