Pathogenic organism pollution control method and application thereof

A technology of pathogenic microorganisms and biological ceramsite, which is applied in the field of water treatment, can solve problems such as the unstable number of Bdellovibrio and the difficulty in producing pathogenic microorganism pollution prevention and control effects, and achieve the effect of avoiding environmental and ecological risks

Inactive Publication Date: 2018-05-22
东莞松山湖高新技术产业开发区循环经济发展促进中心东莞生态产业园区循环经济发展促进中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, due to the uncertain frequency of rainwater and the large change in the number of Bdellovibrio host cells brought by rainwater scouring, the number of Bdellovibrio in natural landscape water environments is unstable, and it is difficult to produce stable pathogenic microbial pollution control effects

Method used

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  • Pathogenic organism pollution control method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Prepare the ceramsite carrier: select biological ceramsite with a diameter of 10mm, a micropore diameter of about 300 microns, and a porosity greater than 40%, soak it in tap water for 24 hours, then clean it with tap water, and dry it at 105°C for backup. use;

[0036] 2. Preparation of host nutrient solution: 10.0 g of tryptone, 5.0 g of yeast powder, 10.0 g of sodium chloride and 1000 ml of pure water are used to prepare a nutrient solution of the host (non-pathogenic Escherichia coli), which is autoclaved for later use;

[0037] 3. Host nutrient storage: Soak the washed and dried biological ceramsite in the high-pressure sterilized host nutrient solution for 30 minutes, then take it out, dry it at room temperature and then soak it, repeat three times to obtain the pore inner wall for storage Bioceramsite for host nutrition;

[0038] 4. Prepare the host culture medium: non-pathogenic Escherichia coli (obtained by isolation and cultivation in the laboratory accord...

Embodiment 2

[0043] 1. Prepare the ceramsite carrier: select biological ceramsite with a diameter of 15mm, a micropore diameter of about 150 microns, and a porosity of 50%, soak it in tap water for 12 hours, then clean it with tap water, and dry it at 100°C for backup. use;

[0044] 2. Preparation of host nutrient solution: 10.0 g of tryptone, 5.0 g of yeast powder, 10.0 g of sodium chloride and 1000 ml of pure water were used to prepare a nutrient solution for non-pathogenic E. coli, and then autoclaved for later use;

[0045] 3. Nutrients stored by the host: Soak the biological ceramsite after drying and constant weight in the host nutrient solution after autoclaving for 30 minutes, remove it, dry it at room temperature and then soak it, repeat three times to obtain the inner wall of the pore Biological ceramsite attached to host culture substrate;

[0046]4. Prepare the host culture solution: non-pathogenic Escherichia coli (Escherichia coli) is inoculated into the nutrient solution of...

Embodiment 3

[0051] 1. Prepare the ceramsite carrier: select biological ceramsite with a diameter of 15mm, a micropore diameter of about 350 microns, and a porosity of 60%, soak it in tap water for 48 hours, then clean it with tap water, and dry it at 115°C for backup. use;

[0052] 2. Preparation of host nutrient solution: 10.0 g of tryptone, 5.0 g of yeast powder, 10.0 g of sodium chloride and 1000 ml of pure water were used to prepare a nutrient solution for non-pathogenic E. coli, and then autoclaved for later use;

[0053] 3. Nutrients stored by the host: Soak the biological ceramsite after drying and constant weight in the host nutrient solution after autoclaving for 30 minutes, remove it, dry it at room temperature and then soak it, repeat three times to obtain the inner wall of the pore Biological ceramsite attached to host culture substrate;

[0054] 4. Prepare the host culture solution: non-pathogenic Escherichia coli (Escherichia coli) is inoculated into the nutrient solution o...

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Abstract

The invention discloses a pathogenic microorganism pollution control method and an application thereof. The method comprises the following steps: cleaning and drying porous biological ceramsite, impregnating the ceramsite in a host nutrient solution, and airing the impregnated ceramsite in order to store host nutrients in the pores of the ceramsite in advance; inoculating a host culture solution with Bdellovibrio bacteriovorus, and carrying out shake culture on the Bdellovibrio bacteriovorus at 25-35 DEG C to obtain a Bdellovibrio bacteriovorus and host mixed culture solution; immersing the host nutrient-prestored biological ceramsite in the Bdellovibrio bacteriovorus and host mixed culture solution, carrying out shake culture at a 25-35 DEG C constant temperature shaking table to obtain biological ceramsite with the host growing in the pores of the ceramsite and used for supplying basic nutrients to the Bdellovibrio bacteriovorus and stabilizing the quantity of the Bdellovibrio bacteriovorus and with the Bdellovibrio bacteriovorus adhered to the surface of the ceramsite to purify other pathogenic organisms in water; and loading the porous biological ceramsite in a common open container, and standing the open container at the surface layer of sediment to control pathogenic microorganism pollution in the water. The pathogenic microorganism pollution control method is mainly applied in the field of pollution control of landscape water.

Description

technical field [0001] The invention belongs to the technical field of water treatment, and more specifically relates to a prevention and control method and application of pathogenic microorganism pollution. Background technique [0002] The pathogenic microorganisms carried by rainwater or recycled water threaten the health and safety of people around the water environment of the sponge city landscape. The use of phage Bdellovibrio (referred to as Bdellovibrio) to biologically purify pathogenic microorganisms in the landscape water environment can avoid physical (ultraviolet, ultrasonic, etc.) harm. [0003] Bdellovibrio exists widely in various surface water environments, and is harmless to eukaryotic organisms such as humans, animals, and plants. It is a type of bacteria that parasitizes pathogenic bacteria such as Escherichia coli, Shigella, and typhoid bacteria, and can cause host lysis. Domestic research on Bdellovibrio phages began in the mid-1960s. From 1966 to 19...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14C02F3/34C12R1/01
CPCC02F3/34C12N1/20C12N11/14
Inventor 吴鹏举黄娜李萍
Owner 东莞松山湖高新技术产业开发区循环经济发展促进中心东莞生态产业园区循环经济发展促进中心
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