Method for producing amino acids through efficiently treating fermentation broth

A fermentation broth, amino acid technology, applied in the directions of organic chemistry methods, chemical instruments and methods, preparation of organic compounds, etc., can solve the problems of increasing difficulty and the production of three wastes, etc., and achieves simple operation, low yield, and environmental friendliness. Effect

Active Publication Date: 2018-05-29
黑龙江诺潽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the crystallization mother liquor needs ion exchange treatment, which increases the difficulty of subsequent treatment and the production of three wastes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0032] Comprehensively to the preferred selection of each technical feature, the preferred implementation of the method of the present invention is integrated, described as follows:

[0033] (1) Filtrate and filter the fermented liquid with a ceramic microfiltration membrane with a pore size of 0.22 μm to remove the thallus. After the thalline concentration>16%, perform multiple dilutions with water and filter, and the volume of water added for each dilution is 0.1 to 0.5 times that of the original fermented liquid; merge Filtrate; the filtrate obtained by centrifugation of the filtrate raffinate is applied mechanically to the top water of the next kettle time;

[0034] (2) The filtrate obtained in step (1) is carried out decolorization and impurity removal in one of the following two methods:

[0035] a. adding 0.5% activated carbon of the filtrate mass for adsorption to remove impurities and decolorize;

[0036] b. Use an organic membrane with a molecular weight cut-off of ...

Embodiment 1

[0051] A method for efficiently processing fermentation broth to produce L-isoleucine:

[0052] (1) Prepare L-isoleucine fermentation broth according to the method described in the article "Study on L-isoleucine fermentation", "Chinese Modern Applied Pharmacy", 1994(4):26-26.

[0053] (2) Filter the fermented liquid with a ceramic membrane with a pore size of 0.22 μm under a pressure of 0.3 MPa; after the cell concentration is >16%, add water to dilute the concentrated fermented liquid according to 20% of the volume of the original fermented liquid, then continue to filter and repeat the operation Until the amino acid content in the fermented concentrated liquid is less than 0.1% of the amino acid content in the initial fermented liquid; combine the filtrates to obtain filtrate 1-i;

[0054]The supernatant obtained by centrifuging the remaining fermented concentrated liquid can be recycled and applied to the first top water after filtration of the next batch of fermented liqui...

Embodiment 2

[0067] A method for efficiently processing fermentation broth to produce L-leucine:

[0068] Prepare L-leucine fermentation liquid according to the method described in the article "Production of L-leucine by Fermentation Industrial Test Research", "Pharmacy Today", 2000,10(4):22-24.

[0069] (2) Filter the fermented liquid with a ceramic membrane with a pore size of 0.22 μm under a pressure of 0.3 MPa; after the cell concentration is >16%, add water to dilute the concentrated fermented liquid according to 20% of the volume of the original fermented liquid, then continue to filter and repeat the operation Until the amino acid content in the fermentation concentrated liquid is less than 0.1% of the amino acid content in the initial fermentation liquid; combine the filtrates to obtain filtrate 2-i;

[0070] The supernatant obtained by centrifuging the remaining fermented concentrated liquid can be recycled and applied to the first top water after filtration of the next batch of f...

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Abstract

The invention discloses a method for producing amino acids through efficiently treating a fermentation broth. The method comprises (1) filtering a fermentation broth through a ceramic membrane havingpore sizes of 0.2-0.4 micrometers, (2) carrying out decolorization and impurity removal on the filtrate through an organic ultrafiltration membrane or activated carbon, (3) carrying out concentrationon the liquid obtained in the step 2 through a reverse osmosis membrane, (4) carrying out multistage flash evaporation concentration crystallization on the concentrate obtained in the step 3 to obtaina crystalline intermediate product and (5) mixing the crystalline intermediate products with purity of less than 90% and washing the crystalline intermediate products through a crystal washing agent.The method is easy to operate, has high efficiency, saves energy, is environment-friendly, realizes a low cost and solves the problem that the existing amino acid separation and purification method has a low yield and a low cost and produces more three wastes. The prepared amino acid crystal product has purity of more than 95% and a pure yield of more than 85%.

Description

technical field [0001] The invention relates to a process for producing amino acid by fermentation, in particular to a treatment method for fermentation products. technical background [0002] At present, microbial fermentation is widely used in the industrial production of amino acids. In the process of microbial fermentation to produce amino acids, a medium with nutrients needs to be provided. Therefore, in addition to amino acids, the fermentation liquid also contains microorganisms, medium residues, metabolites, etc. Taking the medium composition as an example, it contains inorganic salt, carbon source (glucose, honey), nitrogen source, defoamer (vegetable oil) and so on. Due to the complex composition of the fermentation broth, it needs to be pretreated to obtain high-purity amino acids by means of crystallization. [0003] At present, the ion exchange method is generally used to remove impurities in the fermentation broth at home and abroad. The main process is flocc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C227/40C07C227/42C07C229/08
CPCC07B2200/13C07C227/40C07C227/42C07C229/08Y02P20/10
Inventor 洪永德张聚成刘军范超吴文忠
Owner 黑龙江诺潽生物科技有限公司
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