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CuInS/ZnS quantum dot and method for detecting alkaline phosphatase

A technology of quantum dots and phosphatase, which is applied in the fields of biochemical detection, semiconductor quantum dot synthesis, and analytical chemistry, can solve problems such as insufficient anti-interference ability, large sample demand, and unknown lower limit of detection, so as to reduce physical and mental burden, sample The effect of low requirements and ensuring accuracy

Inactive Publication Date: 2018-05-29
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method requires a large amount of samples, complicated sample pretreatment, low sensitivity, poor selectivity, and insufficient anti-interference ability
Since the complex components in clinical samples (such as whole blood) will interfere with the test results, it can only be used in a system with relatively simple components and color development that is not easily disturbed. Pretreatment of samples such as centrifugation is required before testing. Cannot be used directly to analyze real blood samples
The substrate solution must not contain phenolic substances, and the required amount of serum sample is 100 μL; the lower limit of detection is unknown

Method used

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  • CuInS/ZnS quantum dot and method for detecting alkaline phosphatase
  • CuInS/ZnS quantum dot and method for detecting alkaline phosphatase
  • CuInS/ZnS quantum dot and method for detecting alkaline phosphatase

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preparation example Construction

[0030] The present invention provides a kind of preparation method of CuInS / ZnS quantum dot, comprises the following steps:

[0031] (1) Mixing the copper source compound, the indium source compound and the mercapto fatty acid with water, adjusting the pH value to 8-10 to obtain a mixed solution;

[0032] Among them, Cu 2+ 、In 3+ The molar ratio is 0.015~0.045:0.015~0.120; Cu 2+ 、In 3+ The molar ratio of the sum of moles of mercapto fatty acid to mercapto fatty acid is 0.03-0.06:0.24-0.90;

[0033] (2) Under stirring conditions, mix the mixed solution obtained in step (1) with the sulfur source compound, microwave-assisted heating to raise the temperature of the solution to 90-100°C within 4 minutes after mixing, and maintain the temperature for 1-10 minutes to obtain CuInS core-containing the reaction liquid;

[0034] (3) After the reaction solution obtained in step (2) is cooled to below 50°C, zinc source solution and sulfur source solution are added to the reaction sol...

Embodiment 1

[0054] Synthesis of CuInS / ZnS Quantum Dots by Microwave Assisted Heating

[0055] 0.025mmol InCl at room temperature 3 Dissolve 0.2mmol mercaptopropionic acid (MPA) in 20mL double-distilled water, adjust the pH value to 8 with 1mol / L NaOH solution; then add 0.5mL 0.015mmol CuCl 2 Ammonia solution and 0.3mmol MPA; then add 0.04mmol Na under vigorous stirring 2 S solution, using microwave-assisted heating, the temperature of the solution was raised to 100 ° C within 1 min under 800 W power, and kept for 5 min to obtain CuInS cores. Stop heating and wait until it cools below 50°C, then slowly add 1mL 0.02M Zn(Ac) to the solution 2 solution and 1 mL of 0.02M Na 2 The S solution was heated under 800W microwave again, and the temperature was raised to 100° C. within 1 min, and kept for 5 min. After the reaction was completed, the final product CuInS / ZnS quantum dots were obtained.

Embodiment 2

[0057] Synthesis of CuInS / ZnS Quantum Dots by Microwave Assisted Heating

[0058] 0.06mmol InCl at room temperature 3 Dissolve 0.5mmol mercaptopropionic acid (MPA) in 20mL double-distilled water, adjust the pH value to 10 with 1M NaOH solution; add 0.5mL 0.025mmol CuCl 2 Ammonia solution and 0.4mmol MPA; then add 0.02mmolNa under vigorous stirring 2 S solution, using microwave-assisted heating, the temperature of the solution was raised to 95° C. within 3 minutes under the power of 700 W, and kept for 8 minutes to obtain CuInS nuclei. Stop heating and wait until it cools below 50°C, then slowly add 1mL 0.01M Zn(Ac) to the solution 2 solution and 1 mL of 0.01M Na 2The S solution was heated by microwave at 700W again, and the temperature was raised to 95° C. within 3 minutes, and kept for 8 minutes. After the reaction was completed, the final product CuInS / ZnS quantum dots were obtained.

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Abstract

The invention relates to the technical field of biochemical detection, and discloses a CuInS / ZnS quantum dot and a method for detecting alkaline phosphatase by using the quantum dot. The CuInS / ZnS quantum dot is taken as a probe in a system of hydrolyzing disodium 4-nitrophenylphosphate by alkaline phosphatase, the fluorescence intensity was measured under the condition of <lambada>ex / <lambada>em=405 / 588nm, and the alkaline phosphatase content is obtained through the change of fluorescence intensity and the linear relationship between the alkaline phosphatase and the fluorescence intensity. The optimal excitation wavelength of the CuInS / ZnS quantum dot prepared by the invention is 405nm, and is overlapped with the maximum substrate absorption peak of the PNP (p-nitrophenol), and an inner filtering effect can be generated, so that the purpose of detecting ALP (alkaline phosphatase) is achieved. The detection sensitivity is high, the anti-interference capability is strong, the sample requirement is low, and the direct detection of a serum sample can be realized.

Description

technical field [0001] The invention relates to the technical fields of semiconductor quantum dot synthesis, analytical chemistry and biochemical detection, in particular to a CuInS / ZnS quantum dot and a method for detecting alkaline phosphatase using the quantum dot as a nanometer fluorescent probe. Background technique [0002] At present, the method used in the clinical detection of ALP in serum is colorimetric method. This method uses disodium 4-nitrophenylphosphate (PNPP) as a substrate. In the presence of ALP, PNPP reacts with ALP to produce free phenol (p-nitro Phenol (PNP) and phosphoric acid, phenol reacts with 4-aminoantipyrine in an alkaline solution, and is oxidized by potassium ferricyanide to form a red quinone compound, and its red depth is directly proportional to the ALP activity. Under the condition of 37°C, every 100mL of serum reacted with the substrate for 15 minutes, and the ALP activity to produce 1mg of phenol was 1 King's unit (U). Adjust the zero p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/62G01N21/64B82Y20/00B82Y30/00B82Y40/00
CPCB82Y20/00B82Y30/00B82Y40/00C01P2004/64C01P2006/60C09K11/623G01N21/643G01N2021/6432
Inventor 宋大千张芳梅马品一王兴华孙颖
Owner JILIN UNIV
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